##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccII AgeI Aor51HI ApaI
AscI Asp718I BalI BbrPI BciVI
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BsrBI BsrFI BssHII BstUI
BstXI Cfr10I ClaI CpoI EaeI
EagI EclXI Eco47III Eco52I FseI
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SpeI Sse8387I StuI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 4528 GACGTC GACGT^C
AccIII 2016 TCCGGA T^CCGGA
AclI 4391 AACGTT AA^CGTT
AflII 1922 CTTAAG C^TTAAG
AseI 3447 ATTAAT AT^TAAT
AsnI 3447 ATTAAT AT^TAAT
Asp700I 4670 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4899 TGCGCA TGC^GCA
BamHI 1079 GGATCC G^GATCC
BbeI 1927 GGCGCC GGCGC^C
BcgI 3948 CGACAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1922 CTTAAG C^TTAAG
BglI 4968 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2016 TCCGGA [AT]^CCGG[AT]
BseAI 2016 TCCGGA T^CCGGA
BsmBI 1508 CGTCTC CGTCTC(1/5)
BspEI 2016 TCCGGA T^CCGGA
Bst1107I 233 GTATAC GTA^TAC
BstBI 2425 TTCGAA TT^CGAA
BstEII 2929 GGTCACC G^GT[ACGT]ACC
BstPI 2929 GGTCACC G^GT[ACGT]ACC
BstZ17I 233 GTATAC GTA^TAC
DrdI 4507 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1109 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2929 GGTCACC G^GT[ACGT]ACC
EcoRI 2232 GAATTC G^AATTC
EcoRV 2271 GATATC GAT^ATC
EcoT22I 1492 ATGCAT ATGCA^T
FauI 1679 CCCGC CCCGC(4/6)
FspI 4899 TGCGCA TGC^GCA
HaeII 1927 GGCGCC [GA]GCGC^[CT]
HgaI 4885 GACGC GACGC(5/10)
HincII 1938 GTCAAC GT[CT]^[GA]AC
HindII 1938 GTCAAC GT[CT]^[GA]AC
KasI 1927 GGCGCC G^GCGCC
MroI 2016 TCCGGA T^CCGGA
NarI 1927 GGCGCC GG^CGCC
NdeI 4779 CATATG CA^TATG
NsiI 1492 ATGCAT ATGCA^T
NspV 2425 TTCGAA TT^CGAA
PflMI 142 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3447 ATTAAT AT^TAAT
Psp1406I 4391 AACGTT AA^CGTT
SfoI 1927 GGCGCC GGC^GCC
SfuI 2425 TTCGAA TT^CGAA
SmaI 1735 CCCGGG CCC^GGG
SphI 128 GCATGC GCATG^C
SwaI 5257 ATTTAAAT ATTT^AAAT
Van91I 142 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1735 CCCGGG C^CCGGG
XmaI 1735 CCCGGG C^CCGGG
XmnI 4670 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 194 GTAGAC GT^[AC][GT]AC
233 GTATAC
AcyI 1927 GGCGCC G[GA]^CG[CT]C
4528 GACGTC
AflIII 1044 ACATGT A^C[GA][CT]GT
3127 ACATGT
AhdI 1055 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2511 GACTGAGGGTC
Alw44I 265 GTGCAC G^TGCAC
2741 GTGCAC
AocI 398 CCTTAGG CC^T[ACGT]AGG
3053 CCTTAGG
ApaLI 265 GTGCAC G^TGCAC
2741 GTGCAC
AspEI 1055 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2511 GACTGAGGGTC
BclI 2875 TGATCA T^GATCA
4416 TGATCA
BglII 578 AGATCT A^GATCT
3420 AGATCT
BlpI 3656 GCTCAGC GC^T[ACGT]AGC
4117 GCTCAGC
Bpu1102I 3656 GCTCAGC GC^T[ACGT]AGC
4117 GCTCAGC
BsaHI 1927 GGCGCC G[GA]^CG[CT]C
4528 GACGTC
Bsp1407I 2473 TGTACA T^GTACA
2497 TGTACA
BspHI 2854 TCATGA T^CATGA
3464 TCATGA
BspLU11I 1044 ACATGT A^CATGT
3127 ACATGT
BsrGI 2473 TGTACA T^GTACA
2497 TGTACA
BssSI 470 CTCGTG CACGAG(-5/-1)
1580 CTCGTG
Bsu36I 398 CCTTAGG CC^T[ACGT]AGG
3053 CCTTAGG
CelII 3656 GCTCAGC GC^T[ACGT]AGC
4117 GCTCAGC
CfoI 1928 GCGC GCG^C
4900 GCGC
DraI 5258 TTTAAA TTT^AAA
5308 TTTAAA
Eam1105I 1055 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2511 GACTGAGGGTC
Eco81I 398 CCTTAGG CC^T[ACGT]AGG
3053 CCTTAGG
FbaI 2875 TGATCA T^GATCA
4416 TGATCA
HhaI 1928 GCGC GCG^C
4900 GCGC
Hin1I 1927 GGCGCC G[GA]^CG[CT]C
4528 GACGTC
HinP1I 1928 GCGC G^CGC
4900 GCGC
HindIII 2043 AAGCTT A^AGCTT
4716 AAGCTT
NcoI 542 CCATGG C^CATGG
1241 CCATGG
RcaI 2854 TCATGA T^CATGA
3464 TCATGA
SacI 1458 GAGCTC GAGCT^C
4068 GAGCTC
SexAI 2392 ACCTGGT A^CC[AT]GGT
3341 ACCTGGT
SspBI 2473 TGTACA T^GTACA
2497 TGTACA
SspI 214 AATATT AAT^ATT
5262 AATATT
XbaI 207 TCTAGA T^CTAGA
5104 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaI
AvaII AvrII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII