##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII BbrPI BcgI
BciVI BfrI BsaBI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstZ17I
ClaI CpoI DraIII Eam1105I Eco47III
EcoRI FseI FspI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SgrAI SnaBI SphI Sse8387I SspI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 6125 GACGTC GACGT^C
AccI 4567 GTCTAC GT^[AC][GT]AC
AflIII 468 ACATGT A^C[GA][CT]GT
AgeI 4492 ACCGGT A^CCGGT
AocI 2219 CCTAAGG CC^T[ACGT]AGG
BbeI 5563 GGCGCC GGCGC^C
BbsI 4801 GTCTTC GAAGAC(2/6)
BclI 785 TGATCA T^GATCA
BpuAI 4801 GTCTTC GAAGAC(2/6)
BsaWI 4492 ACCGGT [AT]^CCGG[AT]
BspHI 3708 TCATGA T^CATGA
BspLU11I 468 ACATGT A^CATGT
BstXI 4887 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2219 CCTAAGG CC^T[ACGT]AGG
DrdI 2072 GACCCTAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2219 CCTAAGG CC^T[ACGT]AGG
FbaI 785 TGATCA T^GATCA
HpaI 1051 GTTAAC GTT^AAC
KasI 5563 GGCGCC G^GCGCC
KspI 4524 CCGCGG CCGC^GG
NarI 5563 GGCGCC GG^CGCC
PflMI 2773 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4492 ACCGGT A^CCGGT
PshAI 1296 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3708 TCATGA T^CATGA
SacII 4524 CCGCGG CCGC^GG
SexAI 4128 ACCAGGT A^CC[AT]GGT
SfiI 4996 GGCCGACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5563 GGCGCC GGC^GCC
Van91I 2773 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 3358 CGCG CG^CG
4525 CGCG
Asp700I 820 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2461 GAATGACTTC
AspI 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3412 GACAGCGTC
AvrII 1342 CCTAGG C^CTAGG
2075 CCTAGG
BbvCI 5444 CCTCAGC CCTCAGC(-5/-2)
5125 GCTGAGG
BglI 2837 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4997 GCCGACGTGGC
BglII 108 AGATCT A^GATCT
3933 AGATCT
BlnI 1342 CCTAGG C^CTAGG
2075 CCTAGG
BsaAI 749 CACGTA [CT]AC^GT[GA]
3248 TACGTG
BsiEI 3463 CGGCCG CG[GA][CT]^CG
4995 CGGCCG
BsmBI 2621 GAGACG CGTCTC(1/5)
5624 GAGACG
Bsp1407I 463 TGTACA T^GTACA
2789 TGTACA
BsrDI 4981 CATTGC GCAATG(2/0)
5014 CATTGC
BsrGI 463 TGTACA T^GTACA
2789 TGTACA
BssSI 2317 CACGAG CACGAG(-5/-1)
2942 CACGAG
BstBI 3864 TTCGAA TT^CGAA
5118 TTCGAA
BstUI 3358 CGCG CG^CG
4525 CGCG
EagI 3463 CGGCCG C^GGCCG
4995 CGGCCG
EclXI 3463 CGGCCG C^GGCCG
4995 CGGCCG
Eco52I 3463 CGGCCG C^GGCCG
4995 CGGCCG
EcoNI 1914 CCTAGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5224 CCTGGAACAGG
EcoRV 2195 GATATC GAT^ATC
4818 GATATC
EcoT22I 2614 ATGCAT ATGCA^T
2715 ATGCAT
HindIII 1556 AAGCTT A^AGCTT
3395 AAGCTT
MvnI 3358 CGCG CG^CG
4525 CGCG
NaeI 3461 GCCGGC GCC^GGC
4912 GCCGGC
NgoMIV 3461 GCCGGC G^CCGGC
4912 GCCGGC
NsiI 2614 ATGCAT ATGCA^T
2715 ATGCAT
NspV 3864 TTCGAA TT^CGAA
5118 TTCGAA
PflFI 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3412 GACAGCGTC
SapI 4153 GAAGAGC GCTCTTC(1/4)
5575 GAAGAGC
SfuI 3864 TTCGAA TT^CGAA
5118 TTCGAA
SmlI 1495 CTTGAG C^T[CT][GA]AG
2675 CTCAAG
SpeI 313 ACTAGT A^CTAGT
1423 ACTAGT
SspBI 463 TGTACA T^GTACA
2789 TGTACA
Tth111I 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3412 GACAGCGTC
XmnI 820 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2461 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI