################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## Acc65I AccIII AclI AflII AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII BbrPI BcgI BciVI BfrI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstZ17I ClaI CpoI DraIII Eam1105I Eco47III EcoRI FseI FspI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SgrAI SnaBI SphI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 6125 GACGTC GACGT^C AccI 4567 GTCTAC GT^[AC][GT]AC AflIII 468 ACATGT A^C[GA][CT]GT AgeI 4492 ACCGGT A^CCGGT AocI 2219 CCTAAGG CC^T[ACGT]AGG BbeI 5563 GGCGCC GGCGC^C BbsI 4801 GTCTTC GAAGAC(2/6) BclI 785 TGATCA T^GATCA BpuAI 4801 GTCTTC GAAGAC(2/6) BsaWI 4492 ACCGGT [AT]^CCGG[AT] BspHI 3708 TCATGA T^CATGA BspLU11I 468 ACATGT A^CATGT BstXI 4887 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2219 CCTAAGG CC^T[ACGT]AGG DrdI 2072 GACCCTAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2219 CCTAAGG CC^T[ACGT]AGG FbaI 785 TGATCA T^GATCA HpaI 1051 GTTAAC GTT^AAC KasI 5563 GGCGCC G^GCGCC KspI 4524 CCGCGG CCGC^GG NarI 5563 GGCGCC GG^CGCC PflMI 2773 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 4492 ACCGGT A^CCGGT PshAI 1296 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3708 TCATGA T^CATGA SacII 4524 CCGCGG CCGC^GG SexAI 4128 ACCAGGT A^CC[AT]GGT SfiI 4996 GGCCGACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 5563 GGCGCC GGC^GCC Van91I 2773 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 3358 CGCG CG^CG 4525 CGCG Asp700I 820 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2461 GAATGACTTC AspI 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3412 GACAGCGTC AvrII 1342 CCTAGG C^CTAGG 2075 CCTAGG BbvCI 5444 CCTCAGC CCTCAGC(-5/-2) 5125 GCTGAGG BglI 2837 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4997 GCCGACGTGGC BglII 108 AGATCT A^GATCT 3933 AGATCT BlnI 1342 CCTAGG C^CTAGG 2075 CCTAGG BsaAI 749 CACGTA [CT]AC^GT[GA] 3248 TACGTG BsiEI 3463 CGGCCG CG[GA][CT]^CG 4995 CGGCCG BsmBI 2621 GAGACG CGTCTC(1/5) 5624 GAGACG Bsp1407I 463 TGTACA T^GTACA 2789 TGTACA BsrDI 4981 CATTGC GCAATG(2/0) 5014 CATTGC BsrGI 463 TGTACA T^GTACA 2789 TGTACA BssSI 2317 CACGAG CACGAG(-5/-1) 2942 CACGAG BstBI 3864 TTCGAA TT^CGAA 5118 TTCGAA BstUI 3358 CGCG CG^CG 4525 CGCG EagI 3463 CGGCCG C^GGCCG 4995 CGGCCG EclXI 3463 CGGCCG C^GGCCG 4995 CGGCCG Eco52I 3463 CGGCCG C^GGCCG 4995 CGGCCG EcoNI 1914 CCTAGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5224 CCTGGAACAGG EcoRV 2195 GATATC GAT^ATC 4818 GATATC EcoT22I 2614 ATGCAT ATGCA^T 2715 ATGCAT HindIII 1556 AAGCTT A^AGCTT 3395 AAGCTT MvnI 3358 CGCG CG^CG 4525 CGCG NaeI 3461 GCCGGC GCC^GGC 4912 GCCGGC NgoMIV 3461 GCCGGC G^CCGGC 4912 GCCGGC NsiI 2614 ATGCAT ATGCA^T 2715 ATGCAT NspV 3864 TTCGAA TT^CGAA 5118 TTCGAA PflFI 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3412 GACAGCGTC SapI 4153 GAAGAGC GCTCTTC(1/4) 5575 GAAGAGC SfuI 3864 TTCGAA TT^CGAA 5118 TTCGAA SmlI 1495 CTTGAG C^T[CT][GA]AG 2675 CTCAAG SpeI 313 ACTAGT A^CTAGT 1423 ACTAGT SspBI 463 TGTACA T^GTACA 2789 TGTACA Tth111I 2388 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3412 GACAGCGTC XmnI 820 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2461 GAATGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI