################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Aor51HI AscI Asp700I Asp718I AspI BanI BbeI BbrPI BcgI BcnI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII BstBI Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI Hin1I KasI KpnI MamI MluI MroI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 88 CGCG CG^CG AclI 4708 AACGTT AA^CGTT AocI 3108 CCTTAGG CC^T[ACGT]AGG AviII 2405 TGCGCA TGC^GCA AvrII 4405 CCTAGG C^CTAGG BbvCI 2370 GCTGAGG CCTCAGC(-5/-2) BclI 2070 TGATCA T^GATCA BglI 518 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2627 AGATCT A^GATCT BlnI 4405 CCTAGG C^CTAGG BlpI 5731 GCTCAGC GC^T[ACGT]AGC Bpu1102I 5731 GCTCAGC GC^T[ACGT]AGC BsaAI 60 TACGTG [CT]AC^GT[GA] BsaWI 690 ACCGGA [AT]^CCGG[AT] BsmBI 5475 GAGACG CGTCTC(1/5) Bst1107I 4603 GTATAC GTA^TAC BstEII 686 GGTGACC G^GT[ACGT]ACC BstPI 686 GGTGACC G^GT[ACGT]ACC BstUI 88 CGCG CG^CG BstZ17I 4603 GTATAC GTA^TAC Bsu36I 3108 CCTTAGG CC^T[ACGT]AGG CelII 5731 GCTCAGC GC^T[ACGT]AGC Eco81I 3108 CCTTAGG CC^T[ACGT]AGG EcoO65I 686 GGTGACC G^GT[ACGT]ACC EcoRI 2606 GAATTC G^AATTC FbaI 2070 TGATCA T^GATCA FspI 2405 TGCGCA TGC^GCA HaeII 6425 AGCGCC [GA]GCGC^[CT] HapII 691 CCGG C^CGG HgaI 5919 GACGC GACGC(5/10) HpaI 1507 GTTAAC GTT^AAC HpaII 691 CCGG C^CGG KspI 87 CCGCGG CCGC^GG MspI 691 CCGG C^CGG MvnI 88 CGCG CG^CG NheI 4655 GCTAGC G^CTAGC PaeR7I 4799 CTCGAG C^TCGAG Psp1406I 4708 AACGTT AA^CGTT SacII 87 CCGCGG CCGC^GG ScaI 4067 AGTACT AGT^ACT SphI 2494 GCATGC GCATG^C XhoI 4799 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### ApaI 4847 GGGCCC GGGCC^C 5303 GGGCCC AseI 1441 ATTAAT AT^TAAT 3072 ATTAAT AsnI 1441 ATTAAT AT^TAAT 3072 ATTAAT AvaI 231 CTCGGG C^[CT]CG[GA]G 4799 CTCGAG BamHI 1919 GGATCC G^GATCC 5014 GGATCC BsmI 1867 GAATGC GAATGC(1/-1) 2648 GCATTC BsoBI 231 CTCGGG C^[CT]CG[GA]G 4799 CTCGAG BspHI 612 TCATGA T^CATGA 957 TCATGA BssSI 5325 CTCGTG CACGAG(-5/-1) 5506 CTCGTG CfoI 2406 GCGC GCG^C 6426 GCGC DraIII 2334 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG 4492 CACACTGTG DrdI 6 GACAAACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 392 GACACACATGTC HhaI 2406 GCGC GCG^C 6426 GCGC HinP1I 2406 GCGC G^CGC 6426 GCGC MfeI 3308 CAATTG C^AATTG 3377 CAATTG MunI 3308 CAATTG C^AATTG 3377 CAATTG PshAI 5450 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5477 GACGTGGGTC PshBI 1441 ATTAAT AT^TAAT 3072 ATTAAT RcaI 612 TCATGA T^CATGA 957 TCATGA SapI 577 GCTCTTC GCTCTTC(1/4) 3834 GCTCTTC XbaI 3566 TCTAGA T^CTAGA 4205 TCTAGA XcmI 2003 CCAGGTTGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5639 CCATTTTCTCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AhdI AluI Alw44I AlwI AlwNI ApaLI ApoI AspEI AspHI AvaII BalI BanII BbsI BbvI BciVI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII