##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI AscI Asp700I Asp718I AspI
BanI BbeI BbrPI BcgI BcnI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BstBI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI Hin1I KasI KpnI MamI
MluI MroI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 88 CGCG CG^CG
AclI 4708 AACGTT AA^CGTT
AocI 3108 CCTTAGG CC^T[ACGT]AGG
AviII 2405 TGCGCA TGC^GCA
AvrII 4405 CCTAGG C^CTAGG
BbvCI 2370 GCTGAGG CCTCAGC(-5/-2)
BclI 2070 TGATCA T^GATCA
BglI 518 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2627 AGATCT A^GATCT
BlnI 4405 CCTAGG C^CTAGG
BlpI 5731 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5731 GCTCAGC GC^T[ACGT]AGC
BsaAI 60 TACGTG [CT]AC^GT[GA]
BsaWI 690 ACCGGA [AT]^CCGG[AT]
BsmBI 5475 GAGACG CGTCTC(1/5)
Bst1107I 4603 GTATAC GTA^TAC
BstEII 686 GGTGACC G^GT[ACGT]ACC
BstPI 686 GGTGACC G^GT[ACGT]ACC
BstUI 88 CGCG CG^CG
BstZ17I 4603 GTATAC GTA^TAC
Bsu36I 3108 CCTTAGG CC^T[ACGT]AGG
CelII 5731 GCTCAGC GC^T[ACGT]AGC
Eco81I 3108 CCTTAGG CC^T[ACGT]AGG
EcoO65I 686 GGTGACC G^GT[ACGT]ACC
EcoRI 2606 GAATTC G^AATTC
FbaI 2070 TGATCA T^GATCA
FspI 2405 TGCGCA TGC^GCA
HaeII 6425 AGCGCC [GA]GCGC^[CT]
HapII 691 CCGG C^CGG
HgaI 5919 GACGC GACGC(5/10)
HpaI 1507 GTTAAC GTT^AAC
HpaII 691 CCGG C^CGG
KspI 87 CCGCGG CCGC^GG
MspI 691 CCGG C^CGG
MvnI 88 CGCG CG^CG
NheI 4655 GCTAGC G^CTAGC
PaeR7I 4799 CTCGAG C^TCGAG
Psp1406I 4708 AACGTT AA^CGTT
SacII 87 CCGCGG CCGC^GG
ScaI 4067 AGTACT AGT^ACT
SphI 2494 GCATGC GCATG^C
XhoI 4799 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 4847 GGGCCC GGGCC^C
5303 GGGCCC
AseI 1441 ATTAAT AT^TAAT
3072 ATTAAT
AsnI 1441 ATTAAT AT^TAAT
3072 ATTAAT
AvaI 231 CTCGGG C^[CT]CG[GA]G
4799 CTCGAG
BamHI 1919 GGATCC G^GATCC
5014 GGATCC
BsmI 1867 GAATGC GAATGC(1/-1)
2648 GCATTC
BsoBI 231 CTCGGG C^[CT]CG[GA]G
4799 CTCGAG
BspHI 612 TCATGA T^CATGA
957 TCATGA
BssSI 5325 CTCGTG CACGAG(-5/-1)
5506 CTCGTG
CfoI 2406 GCGC GCG^C
6426 GCGC
DraIII 2334 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
4492 CACACTGTG
DrdI 6 GACAAACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
392 GACACACATGTC
HhaI 2406 GCGC GCG^C
6426 GCGC
HinP1I 2406 GCGC G^CGC
6426 GCGC
MfeI 3308 CAATTG C^AATTG
3377 CAATTG
MunI 3308 CAATTG C^AATTG
3377 CAATTG
PshAI 5450 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5477 GACGTGGGTC
PshBI 1441 ATTAAT AT^TAAT
3072 ATTAAT
RcaI 612 TCATGA T^CATGA
957 TCATGA
SapI 577 GCTCTTC GCTCTTC(1/4)
3834 GCTCTTC
XbaI 3566 TCTAGA T^CTAGA
4205 TCTAGA
XcmI 2003 CCAGGTTGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5639 CCATTTTCTCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaLI ApoI AspEI AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII