################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Aor51HI AscI Asp718I AviII BbeI BbrPI BglI BglII BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BspEI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII Cfr10I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HgaI Hin1I KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SphI Sse8387I SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 2872 GTCTAC GT^[AC][GT]AC AccII 5440 CGCG CG^CG AclI 1320 AACGTT AA^CGTT AflIII 2525 ACATGT A^C[GA][CT]GT Alw44I 3435 GTGCAC G^TGCAC AocI 469 CCTGAGG CC^T[ACGT]AGG ApaI 5139 GGGCCC GGGCC^C ApaLI 3435 GTGCAC G^TGCAC AspI 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 989 CCTAGG C^CTAGG BbvCI 329 GCTGAGG CCTCAGC(-5/-2) BcnI 4471 CCGGG CC^[GC]GG BlnI 989 CCTAGG C^CTAGG BsaAI 5167 TACGTA [CT]AC^GT[GA] BsmBI 1359 CGTCTC CGTCTC(1/5) BspDI 3897 ATCGAT AT^CGAT BspLU11I 2525 ACATGT A^CATGT BspMI 124 GCAGGT ACCTGC(4/8) BstBI 1530 TTCGAA TT^CGAA BstUI 5440 CGCG CG^CG Bsu36I 469 CCTGAGG CC^T[ACGT]AGG ClaI 3897 ATCGAT AT^CGAT DraIII 4920 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 469 CCTGAGG CC^T[ACGT]AGG EcoNI 4997 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 3346 GCGGG CCCGC(4/6) HpaI 3608 GTTAAC GTT^AAC KspI 5439 CCGCGG CCGC^GG MvnI 5440 CGCG CG^CG NciI 4471 CCGGG CC^[GC]GG NcoI 3632 CCATGG C^CATGG NheI 1718 GCTAGC G^CTAGC NspV 1530 TTCGAA TT^CGAA PaeR7I 3120 CTCGAG C^TCGAG PflFI 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3856 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1018 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1320 AACGTT AA^CGTT SacII 5439 CCGCGG CCGC^GG SfuI 1530 TTCGAA TT^CGAA SnaBI 5167 TACGTA TAC^GTA SpeI 4831 ACTAGT A^CTAGT StuI 706 AGGCCT AGG^CCT Tsp45I 2942 GTCAC ^GT[GC]AC Tth111I 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3856 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3120 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AhdI 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2708 GACCGGATGTC AlwNI 459 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 4610 CAGATTCTG AseI 971 ATTAAT AT^TAAT 1828 ATTAAT AsnI 971 ATTAAT AT^TAAT 1828 ATTAAT AspEI 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2708 GACCGGATGTC BamHI 4040 GGATCC G^GATCC 4340 GGATCC BanI 3159 GGTGCC G^G[CT][GA]CC 4405 GGTGCC BbsI 1345 GAAGAC GAAGAC(2/6) 1026 GTCTTC BciVI 547 GGATAC GTATCC(6/5) 993 GGATAC BclI 1158 TGATCA T^GATCA 5033 TGATCA BpuAI 1345 GAAGAC GAAGAC(2/6) 1026 GTCTTC BsaI 378 GAGACC GGTCTC(1/5) 3716 GAGACC BsaWI 2709 ACCGGA [AT]^CCGG[AT] 5071 ACCGGA BspHI 414 TCATGA T^CATGA 1990 TCATGA BsrBI 3336 GAGCGG CCGCTC(-3/-3) 4529 GAGCGG CfoI 222 GCGC GCG^C 575 GCGC DraII 1018 AGGACCT [GA]G^G[ACGT]CC[CT] 5139 GGGCCCT DrdI 1348 GACAAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2933 GACACCGTTGTC DsaI 3632 CCATGG C^C[GA][CT]GG 5439 CCGCGG Eam1105I 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2708 GACCGGATGTC EcoO109I 1018 AGGACCT [GA]G^G[ACGT]CC[CT] 5139 GGGCCCT EcoT22I 3456 ATGCAT ATGCA^T 4161 ATGCAT FbaI 1158 TGATCA T^GATCA 5033 TGATCA HaeII 221 AGCGCC [GA]GCGC^[CT] 574 AGCGCC HhaI 222 GCGC GCG^C 575 GCGC HinP1I 222 GCGC G^CGC 575 GCGC NsiI 3456 ATGCAT ATGCA^T 4161 ATGCAT PshBI 971 ATTAAT AT^TAAT 1828 ATTAAT RcaI 414 TCATGA T^CATGA 1990 TCATGA SacI 3123 GAGCTC GAGCT^C 4871 GAGCTC SspI 4103 AATATT AAT^ATT 5590 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbvI BcgI BfaI BfrI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI