################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccI AclI Aor51HI ApaI AscI Asp700I Asp718I BbeI BglII BsaBI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshAI Psp1406I RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccIII 934 TCCGGA T^CCGGA AcyI 1756 GGCGTC G[GA]^CG[CT]C AflII 3331 CTTAAG C^TTAAG AgeI 4341 ACCGGT A^CCGGT AocI 3406 CCTCAGG CC^T[ACGT]AGG AviII 4300 TGCGCA TGC^GCA BbvCI 3916 GCTGAGG CCTCAGC(-5/-2) BclI 3812 TGATCA T^GATCA BfrI 3331 CTTAAG C^TTAAG BsaHI 1756 GGCGTC G[GA]^CG[CT]C BseAI 934 TCCGGA T^CCGGA BsmBI 2321 CGTCTC CGTCTC(1/5) BspEI 934 TCCGGA T^CCGGA BssSI 4077 CACGAG CACGAG(-5/-1) Bsu36I 3406 CCTCAGG CC^T[ACGT]AGG Eco81I 3406 CCTCAGG CC^T[ACGT]AGG EcoNI 3748 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 3812 TGATCA T^GATCA FspI 4300 TGCGCA TGC^GCA Hin1I 1756 GGCGTC G[GA]^CG[CT]C HindIII 458 AAGCTT A^AGCTT HpaI 4123 GTTAAC GTT^AAC MroI 934 TCCGGA T^CCGGA PinAI 4341 ACCGGT A^CCGGT PvuI 1597 CGATCG CGAT^CG SapI 2791 GCTCTTC GCTCTTC(1/4) SmaI 3173 CCCGGG CCC^GGG SspI 1967 AATATT AAT^ATT XbaI 2897 TCTAGA T^CTAGA XmaCI 3173 CCCGGG C^CCGGG XmaI 3173 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 3684 CGCG CG^CG 4295 CGCG AhdI 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2348 GACAGATGGTC Alw44I 2214 GTGCAC G^TGCAC 3377 GTGCAC ApaLI 2214 GTGCAC G^TGCAC 3377 GTGCAC AseI 1832 ATTAAT AT^TAAT 2154 ATTAAT AsnI 1832 ATTAAT AT^TAAT 2154 ATTAAT AspEI 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2348 GACAGATGGTC AspI 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2316 GACCCCGTC AvrII 1826 CCTAGG C^CTAGG 2980 CCTAGG BamHI 2181 GGATCC G^GATCC 3107 GGATCC BbrPI 1434 CACGTG CAC^GTG 4304 CACGTG BcgI 1723 CGACATCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3579 GCATTTTGCTCG BlnI 1826 CCTAGG C^CTAGG 2980 CCTAGG BlpI 1744 GCTGAGC GC^T[ACGT]AGC 2193 GCTCAGC Bpu1102I 1744 GCTGAGC GC^T[ACGT]AGC 2193 GCTCAGC BsiEI 1593 CGACCG CG[GA][CT]^CG 1597 CGATCG BsmI 89 GAATGC GAATGC(1/-1) 4244 GAATGC BsrBI 1138 GAGCGG CCGCTC(-3/-3) 4357 GAGCGG BsrFI 1004 GCCGGT [GA]^CCGG[CT] 4341 ACCGGT BssHII 3683 GCGCGC G^CGCGC 4294 GCGCGC BstAPI 3606 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4107 GCAAATGCTGC BstUI 3684 CGCG CG^CG 4295 CGCG CelII 1744 GCTGAGC GC^T[ACGT]AGC 2193 GCTCAGC Cfr10I 1004 GCCGGT [GA]^CCGG[CT] 4341 ACCGGT DraI 2511 TTTAAA TTT^AAA 3298 TTTAAA DraIII 3834 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4033 CACACTGTG Eam1105I 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2348 GACAGATGGTC EcoRI 1877 GAATTC G^AATTC 4466 GAATTC MvnI 3684 CGCG CG^CG 4295 CGCG NcoI 187 CCATGG C^CATGG 2701 CCATGG PflFI 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2316 GACCCCGTC PmaCI 1434 CACGTG CAC^GTG 4304 CACGTG PmlI 1434 CACGTG CAC^GTG 4304 CACGTG PshBI 1832 ATTAAT AT^TAAT 2154 ATTAAT PstI 3357 CTGCAG CTGCA^G 4264 CTGCAG SacI 551 GAGCTC GAGCT^C 2937 GAGCTC ScaI 127 AGTACT AGT^ACT 3790 AGTACT SphI 3606 GCATGC GCATG^C 4535 GCATGC Tth111I 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2316 GACCCCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NheI NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII