##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AclI Aor51HI
ApaI AscI Asp700I Asp718I BbeI
BglII BsaBI BsiWI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FseI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PmeI PshAI
Psp1406I RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 934 TCCGGA T^CCGGA
AcyI 1756 GGCGTC G[GA]^CG[CT]C
AflII 3331 CTTAAG C^TTAAG
AgeI 4341 ACCGGT A^CCGGT
AocI 3406 CCTCAGG CC^T[ACGT]AGG
AviII 4300 TGCGCA TGC^GCA
BbvCI 3916 GCTGAGG CCTCAGC(-5/-2)
BclI 3812 TGATCA T^GATCA
BfrI 3331 CTTAAG C^TTAAG
BsaHI 1756 GGCGTC G[GA]^CG[CT]C
BseAI 934 TCCGGA T^CCGGA
BsmBI 2321 CGTCTC CGTCTC(1/5)
BspEI 934 TCCGGA T^CCGGA
BssSI 4077 CACGAG CACGAG(-5/-1)
Bsu36I 3406 CCTCAGG CC^T[ACGT]AGG
Eco81I 3406 CCTCAGG CC^T[ACGT]AGG
EcoNI 3748 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3812 TGATCA T^GATCA
FspI 4300 TGCGCA TGC^GCA
Hin1I 1756 GGCGTC G[GA]^CG[CT]C
HindIII 458 AAGCTT A^AGCTT
HpaI 4123 GTTAAC GTT^AAC
MroI 934 TCCGGA T^CCGGA
PinAI 4341 ACCGGT A^CCGGT
PvuI 1597 CGATCG CGAT^CG
SapI 2791 GCTCTTC GCTCTTC(1/4)
SmaI 3173 CCCGGG CCC^GGG
SspI 1967 AATATT AAT^ATT
XbaI 2897 TCTAGA T^CTAGA
XmaCI 3173 CCCGGG C^CCGGG
XmaI 3173 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 3684 CGCG CG^CG
4295 CGCG
AhdI 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2348 GACAGATGGTC
Alw44I 2214 GTGCAC G^TGCAC
3377 GTGCAC
ApaLI 2214 GTGCAC G^TGCAC
3377 GTGCAC
AseI 1832 ATTAAT AT^TAAT
2154 ATTAAT
AsnI 1832 ATTAAT AT^TAAT
2154 ATTAAT
AspEI 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2348 GACAGATGGTC
AspI 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCCCGTC
AvrII 1826 CCTAGG C^CTAGG
2980 CCTAGG
BamHI 2181 GGATCC G^GATCC
3107 GGATCC
BbrPI 1434 CACGTG CAC^GTG
4304 CACGTG
BcgI 1723 CGACATCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3579 GCATTTTGCTCG
BlnI 1826 CCTAGG C^CTAGG
2980 CCTAGG
BlpI 1744 GCTGAGC GC^T[ACGT]AGC
2193 GCTCAGC
Bpu1102I 1744 GCTGAGC GC^T[ACGT]AGC
2193 GCTCAGC
BsiEI 1593 CGACCG CG[GA][CT]^CG
1597 CGATCG
BsmI 89 GAATGC GAATGC(1/-1)
4244 GAATGC
BsrBI 1138 GAGCGG CCGCTC(-3/-3)
4357 GAGCGG
BsrFI 1004 GCCGGT [GA]^CCGG[CT]
4341 ACCGGT
BssHII 3683 GCGCGC G^CGCGC
4294 GCGCGC
BstAPI 3606 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4107 GCAAATGCTGC
BstUI 3684 CGCG CG^CG
4295 CGCG
CelII 1744 GCTGAGC GC^T[ACGT]AGC
2193 GCTCAGC
Cfr10I 1004 GCCGGT [GA]^CCGG[CT]
4341 ACCGGT
DraI 2511 TTTAAA TTT^AAA
3298 TTTAAA
DraIII 3834 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4033 CACACTGTG
Eam1105I 1594 GACCGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2348 GACAGATGGTC
EcoRI 1877 GAATTC G^AATTC
4466 GAATTC
MvnI 3684 CGCG CG^CG
4295 CGCG
NcoI 187 CCATGG C^CATGG
2701 CCATGG
PflFI 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCCCGTC
PmaCI 1434 CACGTG CAC^GTG
4304 CACGTG
PmlI 1434 CACGTG CAC^GTG
4304 CACGTG
PshBI 1832 ATTAAT AT^TAAT
2154 ATTAAT
PstI 3357 CTGCAG CTGCA^G
4264 CTGCAG
SacI 551 GAGCTC GAGCT^C
2937 GAGCTC
ScaI 127 AGTACT AGT^ACT
3790 AGTACT
SphI 3606 GCATGC GCATG^C
4535 GCATGC
Tth111I 688 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII