##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbeI BciVI BlnI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoRI EcoRV FseI HaeII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PmeI PshBI PvuI RcaI RsrII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3332 GTAGAC GT^[AC][GT]AC
AclI 2289 AACGTT AA^CGTT
AflII 3130 CTTAAG C^TTAAG
AgeI 1754 ACCGGT A^CCGGT
AocI 181 CCTGAGG CC^T[ACGT]AGG
AspI 2361 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2142 TGCGCA TGC^GCA
BamHI 3633 GGATCC G^GATCC
BanI 2426 GGCACC G^G[CT][GA]CC
BbrPI 2498 CACGTG CAC^GTG
BcgI 2772 CGAGACCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4576 TGATCA T^GATCA
BfaI 3496 CTAG C^TAG
BfrI 3130 CTTAAG C^TTAAG
BglII 3776 AGATCT A^GATCT
BlpI 4051 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4051 GCTCAGC GC^T[ACGT]AGC
BsaAI 2498 CACGTG [CT]AC^GT[GA]
BsiEI 2684 CGGCCG CG[GA][CT]^CG
Bsp1407I 5005 TGTACA T^GTACA
BsrGI 5005 TGTACA T^GTACA
BssHII 1597 GCGCGC G^CGCGC
Bsu36I 181 CCTGAGG CC^T[ACGT]AGG
CelII 4051 GCTCAGC GC^T[ACGT]AGC
DraIII 1660 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3643 GACCCAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2684 CGGCCG C^GGCCG
EclXI 2684 CGGCCG C^GGCCG
Eco52I 2684 CGGCCG C^GGCCG
Eco81I 181 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2676 ATGCAT ATGCA^T
FbaI 4576 TGATCA T^GATCA
FspI 2142 TGCGCA TGC^GCA
HincII 2296 GTTGAC GT[CT]^[GA]AC
HindII 2296 GTTGAC GT[CT]^[GA]AC
KspI 1790 CCGCGG CCGC^GG
MaeI 3496 CTAG C^TAG
NsiI 2676 ATGCAT ATGCA^T
PaeR7I 856 CTCGAG C^TCGAG
PflFI 2361 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1754 ACCGGT A^CCGGT
PmaCI 2498 CACGTG CAC^GTG
PmlI 2498 CACGTG CAC^GTG
Psp1406I 2289 AACGTT AA^CGTT
SacII 1790 CCGCGG CCGC^GG
SfiI 1779 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2674 GCATGC GCATG^C
SspBI 5005 TGTACA T^GTACA
Tth111I 2361 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 856 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 565 AAATTC [GA]^AATT[CT]
4378 AAATTC
ApoI 565 AAATTC [GA]^AATT[CT]
4378 AAATTC
BsaWI 1754 ACCGGT [AT]^CCGG[AT]
4528 ACCGGA
BspMI 1114 GCAGGT ACCTGC(4/8)
4032 GCAGGT
BssSI 1812 CACGAG CACGAG(-5/-1)
2842 CTCGTG
FauI 2606 CCCGC CCCGC(4/6)
4158 GCGGG
NaeI 598 GCCGGC GCC^GGC
3057 GCCGGC
NgoMIV 598 GCCGGC G^CCGGC
3057 GCCGGC
PshAI 3111 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3905 GACAGTGGTC
SapI 387 GAAGAGC GCTCTTC(1/4)
4613 GCTCTTC
SmaI 152 CCCGGG CCC^GGG
3164 CCCGGG
XmaCI 152 CCCGGG C^CCGGG
3164 CCCGGG
XmaI 152 CCCGGG C^CCGGG
3164 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII