##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AviII BanI BbeI BbrPI BcgI
BfrI BseAI BsiWI BspEI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I FseI FspI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 889 CGCG CG^CG
AhdI 5295 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5096 GTGCAC G^TGCAC
AlwNI 3771 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 5096 GTGCAC G^TGCAC
AspEI 5295 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4189 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5316 CCTAGG C^CTAGG
BclI 4525 TGATCA T^GATCA
BglI 1480 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 355 AGATCT A^GATCT
BlnI 5316 CCTAGG C^CTAGG
BlpI 1560 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1560 GCTCAGC GC^T[ACGT]AGC
BsaBI 4360 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2746 TCCGGT [AT]^CCGG[AT]
BsiEI 2748 CGGTCG CG[GA][CT]^CG
BspDI 5632 ATCGAT AT^CGAT
BssSI 1248 CTCGTG CACGAG(-5/-1)
BstAPI 3407 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3979 GGTCACC G^GT[ACGT]ACC
BstPI 3979 GGTCACC G^GT[ACGT]ACC
BstUI 889 CGCG CG^CG
CelII 1560 GCTCAGC GC^T[ACGT]AGC
ClaI 5632 ATCGAT AT^CGAT
Eam1105I 5295 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3979 GGTCACC G^GT[ACGT]ACC
FbaI 4525 TGATCA T^GATCA
HaeII 2219 AGCGCC [GA]GCGC^[CT]
HpaI 3659 GTTAAC GTT^AAC
MamI 4360 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 889 CGCG CG^CG
NdeI 3673 CATATG CA^TATG
PaeR7I 1207 CTCGAG C^TCGAG
PflFI 4189 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3929 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 5483 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1600 AGTACT AGT^ACT
SexAI 4126 ACCTGGT A^CC[AT]GGT
SmaI 3966 CCCGGG CCC^GGG
SphI 6405 GCATGC GCATG^C
SspI 2676 AATATT AAT^ATT
Tth111I 4189 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1207 CTCGAG C^TCGAG
XmaCI 3966 CCCGGG C^CCGGG
XmaI 3966 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 491 GTCTAC GT^[AC][GT]AC
2434 GTAGAC
AflIII 5397 ACATGT A^C[GA][CT]GT
6536 ACATGT
AocI 1119 CCTGAGG CC^T[ACGT]AGG
6082 CCTCAGG
AseI 2516 ATTAAT AT^TAAT
3696 ATTAAT
AsnI 2516 ATTAAT AT^TAAT
3696 ATTAAT
BbvCI 4785 CCTCAGC CCTCAGC(-5/-2)
108 GCTGAGG
BciVI 5903 GGATAC GTATCC(6/5)
4439 GTATCC
BcnI 3966 CCCGG CC^[GC]GG
3967 CCGGG
BsmBI 2166 CGTCTC CGTCTC(1/5)
264 GAGACG
Bsp1407I 746 TGTACA T^GTACA
5518 TGTACA
BspLU11I 5397 ACATGT A^CATGT
6536 ACATGT
BsrGI 746 TGTACA T^GTACA
5518 TGTACA
BstXI 1195 CCAATACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3835 CCAAGCAGCTGG
Bsu36I 1119 CCTGAGG CC^T[ACGT]AGG
6082 CCTCAGG
CfoI 888 GCGC GCG^C
2220 GCGC
DraII 3929 AGGTCCC [GA]G^G[ACGT]CC[CT]
6136 AGGCCCT
Eco81I 1119 CCTGAGG CC^T[ACGT]AGG
6082 CCTCAGG
EcoNI 4379 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6082 CCTCAGGAAGG
EcoO109I 3929 AGGTCCC [GA]G^G[ACGT]CC[CT]
6136 AGGCCCT
EcoRV 983 GATATC GAT^ATC
2652 GATATC
EcoT22I 5440 ATGCAT ATGCA^T
6403 ATGCAT
FauI 1611 CCCGC CCCGC(4/6)
4826 CCCGC
HapII 2747 CCGG C^CGG
3967 CCGG
HhaI 888 GCGC GCG^C
2220 GCGC
HinP1I 888 GCGC G^CGC
2220 GCGC
HindIII 1269 AAGCTT A^AGCTT
2298 AAGCTT
HpaII 2747 CCGG C^CGG
3967 CCGG
MfeI 2466 CAATTG C^AATTG
2989 CAATTG
MspI 2747 CCGG C^CGG
3967 CCGG
MunI 2466 CAATTG C^AATTG
2989 CAATTG
NciI 3966 CCCGG CC^[GC]GG
3967 CCGGG
NheI 269 GCTAGC G^CTAGC
6076 GCTAGC
NsiI 5440 ATGCAT ATGCA^T
6403 ATGCAT
PshBI 2516 ATTAAT AT^TAAT
3696 ATTAAT
SacI 69 GAGCTC GAGCT^C
2120 GAGCTC
SnaBI 1919 TACGTA TAC^GTA
5426 TACGTA
SspBI 746 TGTACA T^GTACA
5518 TGTACA
StuI 2802 AGGCCT AGG^CCT
2814 AGGCCT
XbaI 3381 TCTAGA T^CTAGA
5309 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BamHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DrdI DsaI
EaeI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI Hin1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI