##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AflII Alw44I ApaI
ApaLI AscI AseI AsnI AvrII
BbrPI BclI BfrI BlnI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRI
FbaI FseI KspI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SbfI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 814 GGTACC G^GTACC
AccII 1398 CGCG CG^CG
AclI 6488 AACGTT AA^CGTT
AgeI 6271 ACCGGT A^CCGGT
Asp718I 814 GGTACC G^GTACC
AspI 1758 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 5307 GCTGAGG CCTCAGC(-5/-2)
BsaWI 6271 ACCGGT [AT]^CCGG[AT]
BsiEI 3977 CGACCG CG[GA][CT]^CG
BsmBI 154 GAGACG CGTCTC(1/5)
BspHI 3889 TCATGA T^CATGA
BspLU11I 4147 ACATGT A^CATGT
BstUI 1398 CGCG CG^CG
DrdI 2895 GACCTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1011 GATATC GAT^ATC
HpaI 2756 GTTAAC GTT^AAC
KpnI 814 GGTACC GGTAC^C
MvnI 1398 CGCG CG^CG
NaeI 2713 GCCGGC GCC^GGC
NcoI 3898 CCATGG C^CATGG
NgoMIV 2713 GCCGGC G^CCGGC
PflFI 1758 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 6271 ACCGGT A^CCGGT
Psp1406I 6488 AACGTT AA^CGTT
RcaI 3889 TCATGA T^CATGA
SfiI 2313 GGCCGTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2956 CCCGGG CCC^GGG
SspI 4494 AATATT AAT^ATT
Tth111I 1758 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2956 CCCGGG C^CCGGG
XmaI 2956 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 62 GGCGCC G[GA]^CG[CT]C
114 GGCGCC
AhdI 156 GACGTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3465 GACTTCCAGTC
AocI 3770 CCTTAGG CC^T[ACGT]AGG
5733 CCTGAGG
AspEI 156 GACGTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3465 GACTTCCAGTC
AviII 4413 TGCGCA TGC^GCA
6508 TGCGCA
BamHI 574 GGATCC G^GATCC
3157 GGATCC
BbeI 62 GGCGCC GGCGC^C
114 GGCGCC
BcgI 1169 CGAGTCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7 GCAGCAGTATCG
BciVI 2206 GGATAC GTATCC(6/5)
5513 GGATAC
BglII 4418 AGATCT A^GATCT
5695 AGATCT
BsaAI 1053 TACGTG [CT]AC^GT[GA]
1283 CACGTA
BsaBI 506 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
569 GATGCGGATC
BsaHI 62 GGCGCC G[GA]^CG[CT]C
114 GGCGCC
BsaI 2077 GAGACC GGTCTC(1/5)
5592 GGTCTC
BstEII 193 GGTCACC G^GT[ACGT]ACC
3629 GGTCACC
BstPI 193 GGTCACC G^GT[ACGT]ACC
3629 GGTCACC
Bsu36I 3770 CCTTAGG CC^T[ACGT]AGG
5733 CCTGAGG
DraIII 428 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3050 CACGAGGTG
Eam1105I 156 GACGTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3465 GACTTCCAGTC
Eco81I 3770 CCTTAGG CC^T[ACGT]AGG
5733 CCTGAGG
EcoO65I 193 GGTCACC G^GT[ACGT]ACC
3629 GGTCACC
EcoT22I 778 ATGCAT ATGCA^T
955 ATGCAT
FspI 4413 TGCGCA TGC^GCA
6508 TGCGCA
HgaI 1574 GCGTC GACGC(5/10)
1814 GCGTC
Hin1I 62 GGCGCC G[GA]^CG[CT]C
114 GGCGCC
KasI 62 GGCGCC G^GCGCC
114 GGCGCC
MamI 506 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
569 GATGCGGATC
NarI 62 GGCGCC GG^CGCC
114 GGCGCC
NdeI 1666 CATATG CA^TATG
5659 CATATG
NsiI 778 ATGCAT ATGCA^T
955 ATGCAT
PaeR7I 3132 CTCGAG C^TCGAG
5479 CTCGAG
PshAI 2258 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3170 GACCAAGGTC
SalI 594 GTCGAC G^TCGAC
5274 GTCGAC
ScaI 2575 AGTACT AGT^ACT
5035 AGTACT
SfoI 62 GGCGCC GGC^GCC
114 GGCGCC
SphI 1955 GCATGC GCATG^C
5257 GCATGC
XcmI 2551 CCAGCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2581 CCACGCAGTCTCTGG
XhoI 3132 CTCGAG C^TCGAG
5479 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI