##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcyI AgeI Aor51HI
AscI AviII AvrII BbeI BcgI
BciVI BclI BglI BglII BlnI
BsaBI BsaHI BsiEI BsiWI BspDI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRI FauI
FbaI FseI FspI HaeII Hin1I
KasI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2358 GGTACC G^GTACC
AccII 3734 CGCG CG^CG
AccIII 4404 TCCGGA T^CCGGA
AhdI 3598 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 57 GGGCCC GGGCC^C
AseI 3115 ATTAAT AT^TAAT
AsnI 3115 ATTAAT AT^TAAT
Asp700I 3339 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2358 GGTACC G^GTACC
AspEI 3598 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2773 CCTCAGC CCTCAGC(-5/-2)
BsaWI 4404 TCCGGA [AT]^CCGG[AT]
BseAI 4404 TCCGGA T^CCGGA
BsmBI 628 CGTCTC CGTCTC(1/5)
BspEI 4404 TCCGGA T^CCGGA
BspLU11I 3289 ACATGT A^CATGT
BsrDI 2818 CATTGC GCAATG(2/0)
BsrFI 3703 GCCGGT [GA]^CCGG[CT]
BstAPI 2813 GCATTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2390 GGTCACC G^GT[ACGT]ACC
BstPI 2390 GGTCACC G^GT[ACGT]ACC
BstUI 3734 CGCG CG^CG
CfoI 3733 GCGC GCG^C
Cfr10I 3703 GCCGGT [GA]^CCGG[CT]
DraI 3060 TTTAAA TTT^AAA
Eam1105I 3598 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2390 GGTCACC G^GT[ACGT]ACC
EcoRV 1836 GATATC GAT^ATC
EcoT22I 1075 ATGCAT ATGCA^T
HhaI 3733 GCGC GCG^C
HinP1I 3733 GCGC G^CGC
HpaI 386 GTTAAC GTT^AAC
KpnI 2358 GGTACC GGTAC^C
MroI 4404 TCCGGA T^CCGGA
MvnI 3734 CGCG CG^CG
NdeI 1650 CATATG CA^TATG
NsiI 1075 ATGCAT ATGCA^T
PshBI 3115 ATTAAT AT^TAAT
PstI 4088 CTGCAG CTGCA^G
SacI 2367 GAGCTC GAGCT^C
SmaI 3798 CCCGGG CCC^GGG
SphI 1933 GCATGC GCATG^C
XbaI 167 TCTAGA T^CTAGA
XmaCI 3798 CCCGGG C^CCGGG
XmaI 3798 CCCGGG C^CCGGG
XmnI 3339 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 3856 CCGC CCGC(-3/-1)
4543 GCGG
AflII 1356 CTTAAG C^TTAAG
2654 CTTAAG
AocI 1900 CCTGAGG CC^T[ACGT]AGG
4645 CCTGAGG
AspI 861 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4012 GACAGAGTC
BamHI 125 GGATCC G^GATCC
1499 GGATCC
BanI 2358 GGTACC G^G[CT][GA]CC
4684 GGTGCC
BanII 57 GGGCCC G[GA]GC[CT]^C
2367 GAGCTC
BbrPI 231 CACGTG CAC^GTG
2763 CACGTG
BfrI 1356 CTTAAG C^TTAAG
2654 CTTAAG
BsaAI 231 CACGTG [CT]AC^GT[GA]
2763 CACGTG
Bsp1407I 2411 TGTACA T^GTACA
2684 TGTACA
BspHI 1840 TCATGA T^CATGA
3551 TCATGA
BsrGI 2411 TGTACA T^GTACA
2684 TGTACA
BstXI 238 CCAGTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3663 CCAGCCTCATGG
BstYI 125 GGATCC [GA]^GATC[CT]
1499 GGATCC
Bsu36I 1900 CCTGAGG CC^T[ACGT]AGG
4645 CCTGAGG
Eco81I 1900 CCTGAGG CC^T[ACGT]AGG
4645 CCTGAGG
HgaI 4514 GACGC GACGC(5/10)
3735 GCGTC
HincII 316 GTTGAC GT[CT]^[GA]AC
386 GTTAAC
HindII 316 GTTGAC GT[CT]^[GA]AC
386 GTTAAC
HindIII 3507 AAGCTT A^AGCTT
4661 AAGCTT
MflI 125 GGATCC [GA]^GATC[CT]
1499 GGATCC
PflFI 861 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4012 GACAGAGTC
PmaCI 231 CACGTG CAC^GTG
2763 CACGTG
PmlI 231 CACGTG CAC^GTG
2763 CACGTG
RcaI 1840 TCATGA T^CATGA
3551 TCATGA
SapI 4408 GAAGAGC GCTCTTC(1/4)
2147 GCTCTTC
SspBI 2411 TGTACA T^GTACA
2684 TGTACA
Tth111I 861 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4012 GACAGAGTC
XhoII 125 GGATCC [GA]^GATC[CT]
1499 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CelII Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI