##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AflIII      
AgeI        AscI        AviII       BalI        BbeI        
BbrPI       BciVI       BfrI        BsaAI       BsaBI       
BsaHI       BsiWI       BsmBI       BsmI        BspDI       
BspLU11I    BssHII      Bst1107I    BstZ17I     ClaI        
CpoI        DrdI        EaeI        EagI        EclXI       
Eco52I      EcoRV       FseI        FspI        Hin1I       
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NgoMIV      NotI        NruI        PacI        
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SfiI        SfoI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1185  GGTACC               G^GTACC
AccI         4455  GTCGAC               GT^[AC][GT]AC
AccII         558  CGCG                 CG^CG
AccIII       3009  TCCGGA               T^CCGGA
AhdI         3085  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2299  GTGCAC               G^TGCAC
Aor51HI      2235  AGCGCT               AGC^GCT
ApaLI        2299  GTGCAC               G^TGCAC
Asp718I      1185  GGTACC               G^GTACC
AspEI        3085  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3104  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2389  CCTAGG               C^CTAGG
BamHI        1024  GGATCC               G^GATCC
BcgI         2918  GCAAAGACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         4890  GCCACCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2389  CCTAGG               C^CTAGG
BsaWI        3009  TCCGGA               [AT]^CCGG[AT]
BseAI        3009  TCCGGA               T^CCGGA
BsiEI        3103  CGACCG               CG[GA][CT]^CG
Bsp1407I     3276  TGTACA               T^GTACA
BspEI        3009  TCCGGA               T^CCGGA
BspHI         855  TCATGA               T^CATGA
BspMI        2583  GCAGGT               ACCTGC(4/8)
BsrBI        2157  CCGCTC               CCGCTC(-3/-3)
BsrFI        4549  ACCGGC               [GA]^CCGG[CT]
BsrGI        3276  TGTACA               T^GTACA
BstBI        2926  TTCGAA               TT^CGAA
BstUI         558  CGCG                 CG^CG
Cfr10I       4549  ACCGGC               [GA]^CCGG[CT]
DraIII       4103  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3085  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2235  AGCGCT               AGC^GCT
EcoNI        3202  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2907  GAATTC               G^AATTC
HgaI         4713  GACGC                GACGC(5/10)
KpnI         1185  GGTACC               GGTAC^C
MroI         3009  TCCGGA               T^CCGGA
MvnI          558  CGCG                 CG^CG
NdeI         5308  CATATG               CA^TATG
NspV         2926  TTCGAA               TT^CGAA
PflFI        3104  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         473  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI          855  TCATGA               T^CATGA
SacI         3118  GAGCTC               GAGCT^C
SalI         4455  GTCGAC               G^TCGAC
SapI          990  GAAGAGC              GCTCTTC(1/4)
SfuI         2926  TTCGAA               TT^CGAA
SmaI         2435  CCCGGG               CCC^GGG
SspBI        3276  TGTACA               T^GTACA
StuI         1378  AGGCCT               AGG^CCT
SwaI         5034  ATTTAAAT             ATTT^AAAT
Tth111I      3104  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2435  CCCGGG               C^CCGGG
XmaI         2435  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI          625  GGGCCC               GGGCC^C
             2061  GGGCCC              
BanI         1185  GGTACC               G^G[CT][GA]CC
             2083  GGTGCC              
BclI           33  TGATCA               T^GATCA
              399  TGATCA              
BcnI         2435  CCCGG                CC^[GC]GG
             2436  CCGGG               
BlpI          976  GCTCAGC              GC^T[ACGT]AGC
             4756  GCTTAGC             
Bpu1102I      976  GCTCAGC              GC^T[ACGT]AGC
             4756  GCTTAGC             
BseRI        4785  CTCCTC               GAGGAG(10/8)
             2259  GAGGAG              
BsgI           56  CTGCAC               GTGCAG(16/14)
             2226  GTGCAG              
BssSI        1384  CACGAG               CACGAG(-5/-1)
             2302  CACGAG              
BstAPI        959  GCAATGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4881  GCAACAAGTGC         
BstEII       2308  GGTGACC              G^GT[ACGT]ACC
             2350  GGTGACC             
BstPI        2308  GGTGACC              G^GT[ACGT]ACC
             2350  GGTGACC             
BstXI         428  CCACTGTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2401  CCAGAACCTTGG        
CelII         976  GCTCAGC              GC^T[ACGT]AGC
             4756  GCTTAGC             
EcoO65I      2308  GGTGACC              G^GT[ACGT]ACC
             2350  GGTGACC             
EcoT22I       248  ATGCAT               ATGCA^T
             3707  ATGCAT              
FauI          613  CCCGC                CCCGC(4/6)
             2156  CCCGC               
FbaI           33  TGATCA               T^GATCA
              399  TGATCA              
HaeII         994  AGCGCC               [GA]GCGC^[CT]
             2235  AGCGCT              
NciI         2435  CCCGG                CC^[GC]GG
             2436  CCGGG               
NcoI          671  CCATGG               C^CATGG
             2034  CCATGG              
NheI         1497  GCTAGC               G^CTAGC
             3397  GCTAGC              
NsiI          248  ATGCAT               ATGCA^T
             3707  ATGCAT              
PstI          788  CTGCAG               CTGCA^G
             2849  CTGCAG              
SexAI        2489  ACCAGGT              A^CC[AT]GGT
             2968  ACCAGGT             
XbaI         2818  TCTAGA               T^CTAGA
             2911  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
Asp700I     AspHI       AvaI        AvaII       BanII       
BbsI        BbvCI       BbvI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PaeR7I      PflMI       
PleI        PshAI       PshBI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoI        XhoII       
XmnI