################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AclI AcyI AflII AflIII AgeI AscI AviII BalI BbeI BbrPI BciVI BfrI BsaAI BsaBI BsaHI BsiWI BsmBI BsmI BspDI BspLU11I BssHII Bst1107I BstZ17I ClaI CpoI DrdI EaeI EagI EclXI Eco52I EcoRV FseI FspI Hin1I HpaI KasI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1185 GGTACC G^GTACC AccI 4455 GTCGAC GT^[AC][GT]AC AccII 558 CGCG CG^CG AccIII 3009 TCCGGA T^CCGGA AhdI 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2299 GTGCAC G^TGCAC Aor51HI 2235 AGCGCT AGC^GCT ApaLI 2299 GTGCAC G^TGCAC Asp718I 1185 GGTACC G^GTACC AspEI 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2389 CCTAGG C^CTAGG BamHI 1024 GGATCC G^GATCC BcgI 2918 GCAAAGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 4890 GCCACCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2389 CCTAGG C^CTAGG BsaWI 3009 TCCGGA [AT]^CCGG[AT] BseAI 3009 TCCGGA T^CCGGA BsiEI 3103 CGACCG CG[GA][CT]^CG Bsp1407I 3276 TGTACA T^GTACA BspEI 3009 TCCGGA T^CCGGA BspHI 855 TCATGA T^CATGA BspMI 2583 GCAGGT ACCTGC(4/8) BsrBI 2157 CCGCTC CCGCTC(-3/-3) BsrFI 4549 ACCGGC [GA]^CCGG[CT] BsrGI 3276 TGTACA T^GTACA BstBI 2926 TTCGAA TT^CGAA BstUI 558 CGCG CG^CG Cfr10I 4549 ACCGGC [GA]^CCGG[CT] DraIII 4103 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2235 AGCGCT AGC^GCT EcoNI 3202 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2907 GAATTC G^AATTC HgaI 4713 GACGC GACGC(5/10) KpnI 1185 GGTACC GGTAC^C MroI 3009 TCCGGA T^CCGGA MvnI 558 CGCG CG^CG NdeI 5308 CATATG CA^TATG NspV 2926 TTCGAA TT^CGAA PflFI 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT] RcaI 855 TCATGA T^CATGA SacI 3118 GAGCTC GAGCT^C SalI 4455 GTCGAC G^TCGAC SapI 990 GAAGAGC GCTCTTC(1/4) SfuI 2926 TTCGAA TT^CGAA SmaI 2435 CCCGGG CCC^GGG SspBI 3276 TGTACA T^GTACA StuI 1378 AGGCCT AGG^CCT SwaI 5034 ATTTAAAT ATTT^AAAT Tth111I 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2435 CCCGGG C^CCGGG XmaI 2435 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### ApaI 625 GGGCCC GGGCC^C 2061 GGGCCC BanI 1185 GGTACC G^G[CT][GA]CC 2083 GGTGCC BclI 33 TGATCA T^GATCA 399 TGATCA BcnI 2435 CCCGG CC^[GC]GG 2436 CCGGG BlpI 976 GCTCAGC GC^T[ACGT]AGC 4756 GCTTAGC Bpu1102I 976 GCTCAGC GC^T[ACGT]AGC 4756 GCTTAGC BseRI 4785 CTCCTC GAGGAG(10/8) 2259 GAGGAG BsgI 56 CTGCAC GTGCAG(16/14) 2226 GTGCAG BssSI 1384 CACGAG CACGAG(-5/-1) 2302 CACGAG BstAPI 959 GCAATGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4881 GCAACAAGTGC BstEII 2308 GGTGACC G^GT[ACGT]ACC 2350 GGTGACC BstPI 2308 GGTGACC G^GT[ACGT]ACC 2350 GGTGACC BstXI 428 CCACTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2401 CCAGAACCTTGG CelII 976 GCTCAGC GC^T[ACGT]AGC 4756 GCTTAGC EcoO65I 2308 GGTGACC G^GT[ACGT]ACC 2350 GGTGACC EcoT22I 248 ATGCAT ATGCA^T 3707 ATGCAT FauI 613 CCCGC CCCGC(4/6) 2156 CCCGC FbaI 33 TGATCA T^GATCA 399 TGATCA HaeII 994 AGCGCC [GA]GCGC^[CT] 2235 AGCGCT NciI 2435 CCCGG CC^[GC]GG 2436 CCGGG NcoI 671 CCATGG C^CATGG 2034 CCATGG NheI 1497 GCTAGC G^CTAGC 3397 GCTAGC NsiI 248 ATGCAT ATGCA^T 3707 ATGCAT PstI 788 CTGCAG CTGCA^G 2849 CTGCAG SexAI 2489 ACCAGGT A^CC[AT]GGT 2968 ACCAGGT XbaI 2818 TCTAGA T^CTAGA 2911 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PshAI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmnI