##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI AscI AviII BalI BbeI
BbrPI BciVI BfrI BsaAI BsaBI
BsaHI BsiWI BsmBI BsmI BspDI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DrdI EaeI EagI EclXI
Eco52I EcoRV FseI FspI Hin1I
HpaI KasI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NgoMIV NotI NruI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1185 GGTACC G^GTACC
AccI 4455 GTCGAC GT^[AC][GT]AC
AccII 558 CGCG CG^CG
AccIII 3009 TCCGGA T^CCGGA
AhdI 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2299 GTGCAC G^TGCAC
Aor51HI 2235 AGCGCT AGC^GCT
ApaLI 2299 GTGCAC G^TGCAC
Asp718I 1185 GGTACC G^GTACC
AspEI 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2389 CCTAGG C^CTAGG
BamHI 1024 GGATCC G^GATCC
BcgI 2918 GCAAAGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4890 GCCACCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2389 CCTAGG C^CTAGG
BsaWI 3009 TCCGGA [AT]^CCGG[AT]
BseAI 3009 TCCGGA T^CCGGA
BsiEI 3103 CGACCG CG[GA][CT]^CG
Bsp1407I 3276 TGTACA T^GTACA
BspEI 3009 TCCGGA T^CCGGA
BspHI 855 TCATGA T^CATGA
BspMI 2583 GCAGGT ACCTGC(4/8)
BsrBI 2157 CCGCTC CCGCTC(-3/-3)
BsrFI 4549 ACCGGC [GA]^CCGG[CT]
BsrGI 3276 TGTACA T^GTACA
BstBI 2926 TTCGAA TT^CGAA
BstUI 558 CGCG CG^CG
Cfr10I 4549 ACCGGC [GA]^CCGG[CT]
DraIII 4103 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3085 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2235 AGCGCT AGC^GCT
EcoNI 3202 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2907 GAATTC G^AATTC
HgaI 4713 GACGC GACGC(5/10)
KpnI 1185 GGTACC GGTAC^C
MroI 3009 TCCGGA T^CCGGA
MvnI 558 CGCG CG^CG
NdeI 5308 CATATG CA^TATG
NspV 2926 TTCGAA TT^CGAA
PflFI 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 855 TCATGA T^CATGA
SacI 3118 GAGCTC GAGCT^C
SalI 4455 GTCGAC G^TCGAC
SapI 990 GAAGAGC GCTCTTC(1/4)
SfuI 2926 TTCGAA TT^CGAA
SmaI 2435 CCCGGG CCC^GGG
SspBI 3276 TGTACA T^GTACA
StuI 1378 AGGCCT AGG^CCT
SwaI 5034 ATTTAAAT ATTT^AAAT
Tth111I 3104 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2435 CCCGGG C^CCGGG
XmaI 2435 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 625 GGGCCC GGGCC^C
2061 GGGCCC
BanI 1185 GGTACC G^G[CT][GA]CC
2083 GGTGCC
BclI 33 TGATCA T^GATCA
399 TGATCA
BcnI 2435 CCCGG CC^[GC]GG
2436 CCGGG
BlpI 976 GCTCAGC GC^T[ACGT]AGC
4756 GCTTAGC
Bpu1102I 976 GCTCAGC GC^T[ACGT]AGC
4756 GCTTAGC
BseRI 4785 CTCCTC GAGGAG(10/8)
2259 GAGGAG
BsgI 56 CTGCAC GTGCAG(16/14)
2226 GTGCAG
BssSI 1384 CACGAG CACGAG(-5/-1)
2302 CACGAG
BstAPI 959 GCAATGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4881 GCAACAAGTGC
BstEII 2308 GGTGACC G^GT[ACGT]ACC
2350 GGTGACC
BstPI 2308 GGTGACC G^GT[ACGT]ACC
2350 GGTGACC
BstXI 428 CCACTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2401 CCAGAACCTTGG
CelII 976 GCTCAGC GC^T[ACGT]AGC
4756 GCTTAGC
EcoO65I 2308 GGTGACC G^GT[ACGT]ACC
2350 GGTGACC
EcoT22I 248 ATGCAT ATGCA^T
3707 ATGCAT
FauI 613 CCCGC CCCGC(4/6)
2156 CCCGC
FbaI 33 TGATCA T^GATCA
399 TGATCA
HaeII 994 AGCGCC [GA]GCGC^[CT]
2235 AGCGCT
NciI 2435 CCCGG CC^[GC]GG
2436 CCGGG
NcoI 671 CCATGG C^CATGG
2034 CCATGG
NheI 1497 GCTAGC G^CTAGC
3397 GCTAGC
NsiI 248 ATGCAT ATGCA^T
3707 ATGCAT
PstI 788 CTGCAG CTGCA^G
2849 CTGCAG
SexAI 2489 ACCAGGT A^CC[AT]GGT
2968 ACCAGGT
XbaI 2818 TCTAGA T^CTAGA
2911 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PshAI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmnI