################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsaAI BsiEI BsmBI Bsp1407I BspLU11I BsrDI BsrGI BssHII BstAPI BstBI CelII CpoI DraIII EagI EclXI Eco52I EcoRV FspI KasI KpnI KspI MluI NarI NdeI NotI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XcmI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2184 GACGTC GACGT^C AccIII 496 TCCGGA T^CCGGA AcyI 2184 GACGTC G[GA]^CG[CT]C AflIII 3706 ACGTGT A^C[GA][CT]GT AhdI 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3920 CCTCAGG CC^T[ACGT]AGG Aor51HI 2328 AGCGCT AGC^GCT ApaI 5275 GGGCCC GGGCC^C AspEI 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 4149 GGATCC G^GATCC BanI 399 GGCACC G^G[CT][GA]CC BbvCI 3537 CCTCAGC CCTCAGC(-5/-2) BglI 5089 GCCTCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4150 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2184 GACGTC G[GA]^CG[CT]C BsaWI 496 TCCGGA [AT]^CCGG[AT] BseAI 496 TCCGGA T^CCGGA BsiWI 1754 CGTACG C^GTACG BspDI 241 ATCGAT AT^CGAT BspEI 496 TCCGGA T^CCGGA BssSI 4456 CTCGTG CACGAG(-5/-1) BstEII 360 GGTGACC G^GT[ACGT]ACC BstPI 360 GGTGACC G^GT[ACGT]ACC Bsu36I 3920 CCTCAGG CC^T[ACGT]AGG ClaI 241 ATCGAT AT^CGAT DrdI 2184 GACGTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2328 AGCGCT AGC^GCT Eco81I 3920 CCTCAGG CC^T[ACGT]AGG EcoO65I 360 GGTGACC G^GT[ACGT]ACC FseI 4930 GGCCGGCC GGCCGG^CC Hin1I 2184 GACGTC G[GA]^CG[CT]C HpaI 2757 GTTAAC GTT^AAC MamI 4150 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1333 CAATTG C^AATTG MroI 496 TCCGGA T^CCGGA MunI 1333 CAATTG C^AATTG NaeI 4931 GCCGGC GCC^GGC NgoMIV 4931 GCCGGC G^CCGGC NheI 2921 GCTAGC G^CTAGC NruI 4830 TCGCGA TCG^CGA PaeR7I 350 CTCGAG C^TCGAG PflMI 252 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 5438 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1277 AGTACT AGT^ACT SexAI 208 ACCAGGT A^CC[AT]GGT SmaI 4613 CCCGGG CCC^GGG Van91I 252 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 350 CTCGAG C^TCGAG XmaCI 4613 CCCGGG C^CCGGG XmaI 4613 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 565 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 5774 CAGTGGCTG AspI 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACAACGTC BalI 1921 TGGCCA TGG^CCA 2281 TGGCCA BciVI 1104 GGATAC GTATCC(6/5) 3929 GGATAC BclI 917 TGATCA T^GATCA 2470 TGATCA BsgI 2932 GTGCAG GTGCAG(16/14) 4810 GTGCAG BspMI 1626 ACCTGC ACCTGC(4/8) 4412 ACCTGC BsrBI 4435 CCGCTC CCGCTC(-3/-3) 1446 GAGCGG BsrFI 1076 GCCGGT [GA]^CCGG[CT] 4931 GCCGGC Bst1107I 1209 GTATAC GTA^TAC 5849 GTATAC BstZ17I 1209 GTATAC GTA^TAC 5849 GTATAC Cfr10I 1076 GCCGGT [GA]^CCGG[CT] 4931 GCCGGC EcoRI 1700 GAATTC G^AATTC 2307 GAATTC EcoT22I 73 ATGCAT ATGCA^T 511 ATGCAT FauI 3092 CCCGC CCCGC(4/6) 1205 GCGGG FbaI 917 TGATCA T^GATCA 2470 TGATCA HaeII 2328 AGCGCT [GA]GCGC^[CT] 5790 AGCGCC HindIII 748 AAGCTT A^AGCTT 2556 AAGCTT MluNI 1921 TGGCCA TGG^CCA 2281 TGGCCA MscI 1921 TGGCCA TGG^CCA 2281 TGGCCA NsiI 73 ATGCAT ATGCA^T 511 ATGCAT NspI 2917 ACATGC [GA]CATG^[CT] 5596 GCATGT PflFI 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACAACGTC SspI 1692 AATATT AAT^ATT 5646 AATATT Tth111I 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACAACGTC XbaI 896 TCTAGA T^CTAGA 5285 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI