##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BcgI BfrI BlnI
BlpI Bpu1102I BsaAI BsiEI BsmBI
Bsp1407I BspLU11I BsrDI BsrGI BssHII
BstAPI BstBI CelII CpoI DraIII
EagI EclXI Eco52I EcoRV FspI
KasI KpnI KspI MluI NarI
NdeI NotI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2184 GACGTC GACGT^C
AccIII 496 TCCGGA T^CCGGA
AcyI 2184 GACGTC G[GA]^CG[CT]C
AflIII 3706 ACGTGT A^C[GA][CT]GT
AhdI 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3920 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2328 AGCGCT AGC^GCT
ApaI 5275 GGGCCC GGGCC^C
AspEI 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4149 GGATCC G^GATCC
BanI 399 GGCACC G^G[CT][GA]CC
BbvCI 3537 CCTCAGC CCTCAGC(-5/-2)
BglI 5089 GCCTCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4150 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2184 GACGTC G[GA]^CG[CT]C
BsaWI 496 TCCGGA [AT]^CCGG[AT]
BseAI 496 TCCGGA T^CCGGA
BsiWI 1754 CGTACG C^GTACG
BspDI 241 ATCGAT AT^CGAT
BspEI 496 TCCGGA T^CCGGA
BssSI 4456 CTCGTG CACGAG(-5/-1)
BstEII 360 GGTGACC G^GT[ACGT]ACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
Bsu36I 3920 CCTCAGG CC^T[ACGT]AGG
ClaI 241 ATCGAT AT^CGAT
DrdI 2184 GACGTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5214 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2328 AGCGCT AGC^GCT
Eco81I 3920 CCTCAGG CC^T[ACGT]AGG
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
FseI 4930 GGCCGGCC GGCCGG^CC
Hin1I 2184 GACGTC G[GA]^CG[CT]C
HpaI 2757 GTTAAC GTT^AAC
MamI 4150 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1333 CAATTG C^AATTG
MroI 496 TCCGGA T^CCGGA
MunI 1333 CAATTG C^AATTG
NaeI 4931 GCCGGC GCC^GGC
NgoMIV 4931 GCCGGC G^CCGGC
NheI 2921 GCTAGC G^CTAGC
NruI 4830 TCGCGA TCG^CGA
PaeR7I 350 CTCGAG C^TCGAG
PflMI 252 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5438 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1277 AGTACT AGT^ACT
SexAI 208 ACCAGGT A^CC[AT]GGT
SmaI 4613 CCCGGG CCC^GGG
Van91I 252 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 350 CTCGAG C^TCGAG
XmaCI 4613 CCCGGG C^CCGGG
XmaI 4613 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 565 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
5774 CAGTGGCTG
AspI 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACAACGTC
BalI 1921 TGGCCA TGG^CCA
2281 TGGCCA
BciVI 1104 GGATAC GTATCC(6/5)
3929 GGATAC
BclI 917 TGATCA T^GATCA
2470 TGATCA
BsgI 2932 GTGCAG GTGCAG(16/14)
4810 GTGCAG
BspMI 1626 ACCTGC ACCTGC(4/8)
4412 ACCTGC
BsrBI 4435 CCGCTC CCGCTC(-3/-3)
1446 GAGCGG
BsrFI 1076 GCCGGT [GA]^CCGG[CT]
4931 GCCGGC
Bst1107I 1209 GTATAC GTA^TAC
5849 GTATAC
BstZ17I 1209 GTATAC GTA^TAC
5849 GTATAC
Cfr10I 1076 GCCGGT [GA]^CCGG[CT]
4931 GCCGGC
EcoRI 1700 GAATTC G^AATTC
2307 GAATTC
EcoT22I 73 ATGCAT ATGCA^T
511 ATGCAT
FauI 3092 CCCGC CCCGC(4/6)
1205 GCGGG
FbaI 917 TGATCA T^GATCA
2470 TGATCA
HaeII 2328 AGCGCT [GA]GCGC^[CT]
5790 AGCGCC
HindIII 748 AAGCTT A^AGCTT
2556 AAGCTT
MluNI 1921 TGGCCA TGG^CCA
2281 TGGCCA
MscI 1921 TGGCCA TGG^CCA
2281 TGGCCA
NsiI 73 ATGCAT ATGCA^T
511 ATGCAT
NspI 2917 ACATGC [GA]CATG^[CT]
5596 GCATGT
PflFI 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACAACGTC
SspI 1692 AATATT AAT^ATT
5646 AATATT
Tth111I 2799 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACAACGTC
XbaI 896 TCTAGA T^CTAGA
5285 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI