################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I ApaLI AscI AviII AvrII BciVI BfrI BlnI BsiWI BspDI Bst1107I BstBI BstZ17I ClaI CpoI EcoNI EcoRV FseI FspI MluI NcoI NheI NruI NspV PacI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 5363 GGTACC G^GTACC AccIII 155 TCCGGA T^CCGGA AhdI 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3566 AGCGCT AGC^GCT Asp700I 3301 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5363 GGTACC G^GTACC AspEI 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 830 GGATCC G^GATCC BbrPI 583 CACGTG CAC^GTG BcgI 298 CGAGCGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 774 TGATCA T^GATCA BsaAI 583 CACGTG [CT]AC^GT[GA] BsaBI 4638 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 155 TCCGGA T^CCGGA Bsp1407I 4888 TGTACA T^GTACA BspEI 155 TCCGGA T^CCGGA BsrDI 1548 CATTGC GCAATG(2/0) BsrGI 4888 TGTACA T^GTACA BssSI 4332 CTCGTG CACGAG(-5/-1) BstEII 2791 GGTGACC G^GT[ACGT]ACC BstPI 2791 GGTGACC G^GT[ACGT]ACC DraIII 2207 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4035 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3566 AGCGCT AGC^GCT EcoO65I 2791 GGTGACC G^GT[ACGT]ACC EcoT14I 5089 CCAAGG C^C[AT][AT]GG EcoT22I 5526 ATGCAT ATGCA^T FbaI 774 TGATCA T^GATCA HindIII 793 AAGCTT A^AGCTT HpaI 2373 GTTAAC GTT^AAC KpnI 5363 GGTACC GGTAC^C MamI 4638 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3173 CAATTG C^AATTG MroI 155 TCCGGA T^CCGGA MunI 3173 CAATTG C^AATTG NaeI 138 GCCGGC GCC^GGC NdeI 4765 CATATG CA^TATG NgoMIV 138 GCCGGC G^CCGGC NsiI 5526 ATGCAT ATGCA^T PaeR7I 3425 CTCGAG C^TCGAG PflMI 3876 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 583 CACGTG CAC^GTG PmlI 583 CACGTG CAC^GTG ScaI 1624 AGTACT AGT^ACT SphI 1704 GCATGC GCATG^C SspBI 4888 TGTACA T^GTACA StyI 5089 CCAAGG C^C[AT][AT]GG Van91I 3876 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3425 CTCGAG C^TCGAG XmnI 3301 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspI 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2763 GACTGGGTC BalI 3374 TGGCCA TGG^CCA 3665 TGGCCA BpmI 2021 CTGGAG CTGGAG(16/14) 2407 CTGGAG BsaWI 155 TCCGGA [AT]^CCGG[AT] 2415 ACCGGA BsmI 1500 GAATGC GAATGC(1/-1) 3391 GAATGC BsrFI 138 GCCGGC [GA]^CCGG[CT] 2281 GCCGGT BssHII 123 GCGCGC G^CGCGC 348 GCGCGC BstXI 1288 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1764 CCATGAGAATGG Cfr10I 138 GCCGGC [GA]^CCGG[CT] 2281 GCCGGT DsaI 206 CCGCGG C^C[GA][CT]GG 213 CCGCGG EcoRI 841 GAATTC G^AATTC 5706 GAATTC KspI 206 CCGCGG CCGC^GG 213 CCGCGG MluNI 3374 TGGCCA TGG^CCA 3665 TGGCCA MscI 3374 TGGCCA TGG^CCA 3665 TGGCCA NotI 161 GCGGCCGC GC^GGCCGC 342 GCGGCCGC PflFI 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2763 GACTGGGTC SacII 206 CCGCGG CCGC^GG 213 CCGCGG Tth111I 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2763 GACTGGGTC XbaI 798 TCTAGA T^CTAGA 2166 TCTAGA XcmI 3653 CCAAGACACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5089 CCAAGGTGACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI