##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AviII AvrII
BciVI BfrI BlnI BsiWI BspDI
Bst1107I BstBI BstZ17I ClaI CpoI
EcoNI EcoRV FseI FspI MluI
NcoI NheI NruI NspV PacI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 5363 GGTACC G^GTACC
AccIII 155 TCCGGA T^CCGGA
AhdI 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3566 AGCGCT AGC^GCT
Asp700I 3301 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5363 GGTACC G^GTACC
AspEI 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 830 GGATCC G^GATCC
BbrPI 583 CACGTG CAC^GTG
BcgI 298 CGAGCGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 774 TGATCA T^GATCA
BsaAI 583 CACGTG [CT]AC^GT[GA]
BsaBI 4638 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 155 TCCGGA T^CCGGA
Bsp1407I 4888 TGTACA T^GTACA
BspEI 155 TCCGGA T^CCGGA
BsrDI 1548 CATTGC GCAATG(2/0)
BsrGI 4888 TGTACA T^GTACA
BssSI 4332 CTCGTG CACGAG(-5/-1)
BstEII 2791 GGTGACC G^GT[ACGT]ACC
BstPI 2791 GGTGACC G^GT[ACGT]ACC
DraIII 2207 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4035 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1674 GACTGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3566 AGCGCT AGC^GCT
EcoO65I 2791 GGTGACC G^GT[ACGT]ACC
EcoT14I 5089 CCAAGG C^C[AT][AT]GG
EcoT22I 5526 ATGCAT ATGCA^T
FbaI 774 TGATCA T^GATCA
HindIII 793 AAGCTT A^AGCTT
HpaI 2373 GTTAAC GTT^AAC
KpnI 5363 GGTACC GGTAC^C
MamI 4638 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3173 CAATTG C^AATTG
MroI 155 TCCGGA T^CCGGA
MunI 3173 CAATTG C^AATTG
NaeI 138 GCCGGC GCC^GGC
NdeI 4765 CATATG CA^TATG
NgoMIV 138 GCCGGC G^CCGGC
NsiI 5526 ATGCAT ATGCA^T
PaeR7I 3425 CTCGAG C^TCGAG
PflMI 3876 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 583 CACGTG CAC^GTG
PmlI 583 CACGTG CAC^GTG
ScaI 1624 AGTACT AGT^ACT
SphI 1704 GCATGC GCATG^C
SspBI 4888 TGTACA T^GTACA
StyI 5089 CCAAGG C^C[AT][AT]GG
Van91I 3876 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3425 CTCGAG C^TCGAG
XmnI 3301 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspI 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACTGGGTC
BalI 3374 TGGCCA TGG^CCA
3665 TGGCCA
BpmI 2021 CTGGAG CTGGAG(16/14)
2407 CTGGAG
BsaWI 155 TCCGGA [AT]^CCGG[AT]
2415 ACCGGA
BsmI 1500 GAATGC GAATGC(1/-1)
3391 GAATGC
BsrFI 138 GCCGGC [GA]^CCGG[CT]
2281 GCCGGT
BssHII 123 GCGCGC G^CGCGC
348 GCGCGC
BstXI 1288 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1764 CCATGAGAATGG
Cfr10I 138 GCCGGC [GA]^CCGG[CT]
2281 GCCGGT
DsaI 206 CCGCGG C^C[GA][CT]GG
213 CCGCGG
EcoRI 841 GAATTC G^AATTC
5706 GAATTC
KspI 206 CCGCGG CCGC^GG
213 CCGCGG
MluNI 3374 TGGCCA TGG^CCA
3665 TGGCCA
MscI 3374 TGGCCA TGG^CCA
3665 TGGCCA
NotI 161 GCGGCCGC GC^GGCCGC
342 GCGGCCGC
PflFI 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACTGGGTC
SacII 206 CCGCGG CCGC^GG
213 CCGCGG
Tth111I 1359 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACTGGGTC
XbaI 798 TCTAGA T^CTAGA
2166 TCTAGA
XcmI 3653 CCAAGACACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5089 CCAAGGTGACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AseI AsnI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI