##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AhdI AscI AspEI
AspI AviII AvrII BalI BbrPI
BcgI BciVI BclI BlnI BseAI
BsiWI BsmBI BsmI BspEI BstEII
BstPI CpoI DrdI Eam1105I EcoO65I
EcoT22I FbaI FseI FspI HgaI
HindIII MfeI MluI MluNI MroI
MscI MunI NaeI NgoMIV NheI
NruI NsiI PacI PaeR7I PflFI
PflMI PmaCI PmlI PshAI PvuI
RsrII SalI SexAI SfiI SgrAI
SnaBI SpeI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 4013 GTATAC GT^[AC][GT]AC
AclI 5010 AACGTT AA^CGTT
AcyI 4884 GGCGCC G[GA]^CG[CT]C
AflIII 4292 ACATGT A^C[GA][CT]GT
AgeI 4811 ACCGGT A^CCGGT
AseI 619 ATTAAT AT^TAAT
AsnI 619 ATTAAT AT^TAAT
BamHI 1024 GGATCC G^GATCC
BbeI 4884 GGCGCC GGCGC^C
BbvCI 2185 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2875 TACGTG [CT]AC^GT[GA]
BsaHI 4884 GGCGCC G[GA]^CG[CT]C
BsaWI 4811 ACCGGT [AT]^CCGG[AT]
BspDI 2459 ATCGAT AT^CGAT
BspHI 4525 TCATGA T^CATGA
BspLU11I 4292 ACATGT A^CATGT
BsrBI 337 CCGCTC CCGCTC(-3/-3)
Bst1107I 4013 GTATAC GTA^TAC
BstBI 2732 TTCGAA TT^CGAA
BstZ17I 4013 GTATAC GTA^TAC
ClaI 2459 ATCGAT AT^CGAT
EcoRV 2098 GATATC GAT^ATC
Hin1I 4884 GGCGCC G[GA]^CG[CT]C
KasI 4884 GGCGCC G^GCGCC
NarI 4884 GGCGCC GG^CGCC
NotI 79 GCGGCCGC GC^GGCCGC
NspV 2732 TTCGAA TT^CGAA
PinAI 4811 ACCGGT A^CCGGT
PmeI 2573 GTTTAAAC GTTT^AAAC
PshBI 619 ATTAAT AT^TAAT
Psp1406I 5010 AACGTT AA^CGTT
RcaI 4525 TCATGA T^CATGA
SacI 2858 GAGCTC GAGCT^C
SfoI 4884 GGCGCC GGC^GCC
SfuI 2732 TTCGAA TT^CGAA
SphI 4447 GCATGC GCATG^C
SspI 4272 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 986 GGTACC G^GTACC
1942 GGTACC
Alw44I 2081 GTGCAC G^TGCAC
3060 GTGCAC
AlwNI 1346 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3178 CAGCTGCTG
Aor51HI 472 AGCGCT AGC^GCT
705 AGCGCT
ApaLI 2081 GTGCAC G^TGCAC
3060 GTGCAC
Asp700I 3327 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3412 GAAATGGTTC
Asp718I 986 GGTACC G^GTACC
1942 GGTACC
BlpI 3123 GCTAAGC GC^T[ACGT]AGC
3231 GCTGAGC
Bpu1102I 3123 GCTAAGC GC^T[ACGT]AGC
3231 GCTGAGC
BsaBI 2098 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4168 GATTTTAATC
BsrDI 2633 CATTGC GCAATG(2/0)
761 GCAATG
BsrFI 1 ACCGGC [GA]^CCGG[CT]
4811 ACCGGT
BssSI 538 CTCGTG CACGAG(-5/-1)
2414 CTCGTG
BstAPI 203 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4451 GCAGAATGTGC
CelII 3123 GCTAAGC GC^T[ACGT]AGC
3231 GCTGAGC
Cfr10I 1 ACCGGC [GA]^CCGG[CT]
4811 ACCGGT
Eco47III 472 AGCGCT AGC^GCT
705 AGCGCT
EcoNI 1858 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2818 CCTGGACCAGG
HpaI 4690 GTTAAC GTT^AAC
4916 GTTAAC
KpnI 986 GGTACC GGTAC^C
1942 GGTACC
MamI 2098 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4168 GATTTTAATC
NdeI 1592 CATATG CA^TATG
2722 CATATG
PvuII 3151 CAGCTG CAG^CTG
3178 CAGCTG
SapI 1907 GCTCTTC GCTCTTC(1/4)
2834 GCTCTTC
SbfI 2605 CCTGCAGG CCTGCA^GG
3516 CCTGCAGG
ScaI 521 AGTACT AGT^ACT
2506 AGTACT
Sse8387I 2605 CCTGCAGG CCTGCA^GG
3516 CCTGCAGG
StuI 294 AGGCCT AGG^CCT
1915 AGGCCT
XmnI 3327 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3412 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BfrI
BglI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI