##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AflII AflIII AgeI AscI
AseI AsnI AvrII BbeI BclI
BfrI BlnI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRI FbaI FseI HpaI
KasI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 238 GACGTC GACGT^C
Acc65I 60 GGTACC G^GTACC
AclI 868 AACGTT AA^CGTT
Alw44I 5408 GTGCAC G^TGCAC
ApaI 4010 GGGCCC GGGCC^C
ApaLI 5408 GTGCAC G^TGCAC
Asp718I 60 GGTACC G^GTACC
AspI 6060 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3555 TGCGCA TGC^GCA
BamHI 4681 GGATCC G^GATCC
BbrPI 4133 CACGTG CAC^GTG
BbvCI 730 CCTCAGC CCTCAGC(-5/-2)
BcgI 6153 GCACAAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4886 GGATAC GTATCC(6/5)
BsiEI 4045 CGGCCG CG[GA][CT]^CG
BssSI 4751 CACGAG CACGAG(-5/-1)
BstAPI 5190 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 3110 GACAGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4045 CGGCCG C^GGCCG
EclXI 4045 CGGCCG C^GGCCG
Eco52I 4045 CGGCCG C^GGCCG
EcoT22I 389 ATGCAT ATGCA^T
FspI 3555 TGCGCA TGC^GCA
HincII 5826 GTTGAC GT[CT]^[GA]AC
HindII 5826 GTTGAC GT[CT]^[GA]AC
KpnI 60 GGTACC GGTAC^C
NsiI 389 ATGCAT ATGCA^T
PflFI 6060 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4133 CACGTG CAC^GTG
PmlI 4133 CACGTG CAC^GTG
Psp1406I 868 AACGTT AA^CGTT
SpeI 723 ACTAGT A^CTAGT
Tth111I 6060 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2555 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 1006 AAATTT [GA]^AATT[CT]
4791 GAATTT
AcyI 238 GACGTC G[GA]^CG[CT]C
1506 GACGCC
AocI 447 CCTAAGG CC^T[ACGT]AGG
3950 CCTCAGG
Aor51HI 1549 AGCGCT AGC^GCT
1894 AGCGCT
ApoI 1006 AAATTT [GA]^AATT[CT]
4791 GAATTT
Asp700I 1608 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3172 GAACATCTTC
BsaAI 790 TACGTG [CT]AC^GT[GA]
4133 CACGTG
BsaHI 238 GACGTC G[GA]^CG[CT]C
1506 GACGCC
Bsp1407I 325 TGTACA T^GTACA
3152 TGTACA
BspHI 5450 TCATGA T^CATGA
6000 TCATGA
BspMI 4903 ACCTGC ACCTGC(4/8)
4347 GCAGGT
BsrFI 1795 GCCGGC [GA]^CCGG[CT]
3187 GCCGGC
BsrGI 325 TGTACA T^GTACA
3152 TGTACA
BstEII 1317 GGTAACC G^GT[ACGT]ACC
5307 GGTGACC
BstPI 1317 GGTAACC G^GT[ACGT]ACC
5307 GGTGACC
Bsu36I 447 CCTAAGG CC^T[ACGT]AGG
3950 CCTCAGG
Cfr10I 1795 GCCGGC [GA]^CCGG[CT]
3187 GCCGGC
Eco47III 1549 AGCGCT AGC^GCT
1894 AGCGCT
Eco81I 447 CCTAAGG CC^T[ACGT]AGG
3950 CCTCAGG
EcoO65I 1317 GGTAACC G^GT[ACGT]ACC
5307 GGTGACC
EcoRV 2226 GATATC GAT^ATC
2700 GATATC
Hin1I 238 GACGTC G[GA]^CG[CT]C
1506 GACGCC
NaeI 1795 GCCGGC GCC^GGC
3187 GCCGGC
NgoMIV 1795 GCCGGC G^CCGGC
3187 GCCGGC
PflMI 2039 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5139 CCATCAGATGG
PpuMI 2904 AGGTCCT [GA]G^G[AT]CC[CT]
3798 GGGACCT
RcaI 5450 TCATGA T^CATGA
6000 TCATGA
SacI 1182 GAGCTC GAGCT^C
1704 GAGCTC
SbfI 2291 CCTGCAGG CCTGCA^GG
3873 CCTGCAGG
SmaI 5 CCCGGG CCC^GGG
39 CCCGGG
SphI 4995 GCATGC GCATG^C
5839 GCATGC
Sse8387I 2291 CCTGCAGG CCTGCA^GG
3873 CCTGCAGG
SspBI 325 TGTACA T^GTACA
3152 TGTACA
SspI 3504 AATATT AAT^ATT
4451 AATATT
Van91I 2039 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5139 CCATCAGATGG
XmaCI 5 CCCGGG C^CCGGG
39 CCCGGG
XmaI 5 CCCGGG C^CCGGG
39 CCCGGG
XmnI 1608 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3172 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII