################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AccII AccIII AclI Alw44I ApaLI AscI AseI AsnI BamHI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII BssSI BstBI BstUI ClaI DraI DraIII EagI EclXI Eco52I EcoRI FseI HpaI KspI MamI MluI MroI MvnI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI SacII SalI SbfI ScaI SexAI SfuI SgrAI SpeI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AhdI 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1493 AGCGCT AGC^GCT Asp700I 1681 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3596 TGCGCA TGC^GCA BbeI 4658 GGCGCC GGCGC^C BbrPI 5035 CACGTG CAC^GTG BcgI 4524 CGACAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2660 GGATAC GTATCC(6/5) BglII 3442 AGATCT A^GATCT BsiEI 2797 CGGTCG CG[GA][CT]^CG BsmBI 1006 GAGACG CGTCTC(1/5) BspHI 250 TCATGA T^CATGA BsrBI 4102 GAGCGG CCGCTC(-3/-3) Bst1107I 908 GTATAC GTA^TAC BstEII 4041 GGTTACC G^GT[ACGT]ACC BstPI 4041 GGTTACC G^GT[ACGT]ACC BstZ17I 908 GTATAC GTA^TAC CpoI 4628 CGGACCG CG^G[AT]CCG DrdI 943 GACACAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1493 AGCGCT AGC^GCT EcoO65I 4041 GGTTACC G^GT[ACGT]ACC EcoRV 148 GATATC GAT^ATC EcoT22I 1754 ATGCAT ATGCA^T FspI 3596 TGCGCA TGC^GCA HindIII 120 AAGCTT A^AGCTT KasI 4658 GGCGCC G^GCGCC MfeI 4479 CAATTG C^AATTG MunI 4479 CAATTG C^AATTG NarI 4658 GGCGCC GG^CGCC NcoI 266 CCATGG C^CATGG NheI 228 GCTAGC G^CTAGC NsiI 1754 ATGCAT ATGCA^T PmaCI 5035 CACGTG CAC^GTG PmlI 5035 CACGTG CAC^GTG RcaI 250 TCATGA T^CATGA RsrII 4628 CGGACCG CG^G[AT]CCG SapI 1978 GAAGAGC GCTCTTC(1/4) SfoI 4658 GGCGCC GGC^GCC SnaBI 978 TACGTA TAC^GTA SspI 2563 AATATT AAT^ATT XmnI 1681 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2278 GGTACC G^GTACC 2414 GGTACC AcsI 986 AAATTT [GA]^AATT[CT] 2944 GAATTT AflII 1380 CTTAAG C^TTAAG 3052 CTTAAG AgeI 162 ACCGGT A^CCGGT 2795 ACCGGT AlwI 2866 GATCC GGATC(4/5) 5107 GATCC ApaI 2574 GGGCCC GGGCC^C 4798 GGGCCC ApoI 986 AAATTT [GA]^AATT[CT] 2944 GAATTT Asp718I 2278 GGTACC G^GTACC 2414 GGTACC AspI 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3831 GACAGGGTC AvrII 5084 CCTAGG C^CTAGG 5363 CCTAGG BclI 3347 TGATCA T^GATCA 4781 TGATCA BfrI 1380 CTTAAG C^TTAAG 3052 CTTAAG BlnI 5084 CCTAGG C^CTAGG 5363 CCTAGG BlpI 3264 GCTTAGC GC^T[ACGT]AGC 4014 GCTGAGC Bpu1102I 3264 GCTTAGC GC^T[ACGT]AGC 4014 GCTGAGC BspLU11I 767 ACATGT A^CATGT 777 ACATGT BsrDI 3153 CATTGC GCAATG(2/0) 382 GCAATG BsrFI 162 ACCGGT [GA]^CCGG[CT] 2795 ACCGGT BstYI 2865 AGATCC [GA]^GATC[CT] 3442 AGATCT CelII 3264 GCTTAGC GC^T[ACGT]AGC 4014 GCTGAGC Cfr10I 162 ACCGGT [GA]^CCGG[CT] 2795 ACCGGT FbaI 3347 TGATCA T^GATCA 4781 TGATCA HaeII 1493 AGCGCT [GA]GCGC^[CT] 4658 GGCGCC HgaI 4732 GACGC GACGC(5/10) 4894 GCGTC HincII 2497 GTCAAC GT[CT]^[GA]AC 4570 GTCAAC HindII 2497 GTCAAC GT[CT]^[GA]AC 4570 GTCAAC KpnI 2278 GGTACC GGTAC^C 2414 GGTACC MflI 2865 AGATCC [GA]^GATC[CT] 3442 AGATCT PflFI 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3831 GACAGGGTC PinAI 162 ACCGGT A^CCGGT 2795 ACCGGT Tth111I 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3831 GACAGGGTC XbaI 1137 TCTAGA T^CTAGA 2404 TCTAGA XhoII 2865 AGATCC [GA]^GATC[CT] 3442 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI