##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AccII       AccIII      AclI        Alw44I      
ApaLI       AscI        AseI        AsnI        BamHI       
BsaBI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BsrGI       BssHII      BssSI       
BstBI       BstUI       ClaI        DraI        DraIII      
EagI        EclXI       Eco52I      EcoRI       FseI        
HpaI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PmeI        
PshAI       PshBI       Psp1406I    PvuI        SacII       
SalI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SpeI        Sse8387I    SspBI       SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AhdI          738  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1493  AGCGCT               AGC^GCT
Asp700I      1681  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         738  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3596  TGCGCA               TGC^GCA
BbeI         4658  GGCGCC               GGCGC^C
BbrPI        5035  CACGTG               CAC^GTG
BcgI         4524  CGACAAGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2660  GGATAC               GTATCC(6/5)
BglII        3442  AGATCT               A^GATCT
BsiEI        2797  CGGTCG               CG[GA][CT]^CG
BsmBI        1006  GAGACG               CGTCTC(1/5)
BspHI         250  TCATGA               T^CATGA
BsrBI        4102  GAGCGG               CCGCTC(-3/-3)
Bst1107I      908  GTATAC               GTA^TAC
BstEII       4041  GGTTACC              G^GT[ACGT]ACC
BstPI        4041  GGTTACC              G^GT[ACGT]ACC
BstZ17I       908  GTATAC               GTA^TAC
CpoI         4628  CGGACCG              CG^G[AT]CCG
DrdI          943  GACACAAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      738  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1493  AGCGCT               AGC^GCT
EcoO65I      4041  GGTTACC              G^GT[ACGT]ACC
EcoRV         148  GATATC               GAT^ATC
EcoT22I      1754  ATGCAT               ATGCA^T
FspI         3596  TGCGCA               TGC^GCA
HindIII       120  AAGCTT               A^AGCTT
KasI         4658  GGCGCC               G^GCGCC
MfeI         4479  CAATTG               C^AATTG
MunI         4479  CAATTG               C^AATTG
NarI         4658  GGCGCC               GG^CGCC
NcoI          266  CCATGG               C^CATGG
NheI          228  GCTAGC               G^CTAGC
NsiI         1754  ATGCAT               ATGCA^T
PmaCI        5035  CACGTG               CAC^GTG
PmlI         5035  CACGTG               CAC^GTG
RcaI          250  TCATGA               T^CATGA
RsrII        4628  CGGACCG              CG^G[AT]CCG
SapI         1978  GAAGAGC              GCTCTTC(1/4)
SfoI         4658  GGCGCC               GGC^GCC
SnaBI         978  TACGTA               TAC^GTA
SspI         2563  AATATT               AAT^ATT
XmnI         1681  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       2278  GGTACC               G^GTACC
             2414  GGTACC              
AcsI          986  AAATTT               [GA]^AATT[CT]
             2944  GAATTT              
AflII        1380  CTTAAG               C^TTAAG
             3052  CTTAAG              
AgeI          162  ACCGGT               A^CCGGT
             2795  ACCGGT              
AlwI         2866  GATCC                GGATC(4/5)
             5107  GATCC               
ApaI         2574  GGGCCC               GGGCC^C
             4798  GGGCCC              
ApoI          986  AAATTT               [GA]^AATT[CT]
             2944  GAATTT              
Asp718I      2278  GGTACC               G^GTACC
             2414  GGTACC              
AspI         3361  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3831  GACAGGGTC           
AvrII        5084  CCTAGG               C^CTAGG
             5363  CCTAGG              
BclI         3347  TGATCA               T^GATCA
             4781  TGATCA              
BfrI         1380  CTTAAG               C^TTAAG
             3052  CTTAAG              
BlnI         5084  CCTAGG               C^CTAGG
             5363  CCTAGG              
BlpI         3264  GCTTAGC              GC^T[ACGT]AGC
             4014  GCTGAGC             
Bpu1102I     3264  GCTTAGC              GC^T[ACGT]AGC
             4014  GCTGAGC             
BspLU11I      767  ACATGT               A^CATGT
              777  ACATGT              
BsrDI        3153  CATTGC               GCAATG(2/0)
              382  GCAATG              
BsrFI         162  ACCGGT               [GA]^CCGG[CT]
             2795  ACCGGT              
BstYI        2865  AGATCC               [GA]^GATC[CT]
             3442  AGATCT              
CelII        3264  GCTTAGC              GC^T[ACGT]AGC
             4014  GCTGAGC             
Cfr10I        162  ACCGGT               [GA]^CCGG[CT]
             2795  ACCGGT              
FbaI         3347  TGATCA               T^GATCA
             4781  TGATCA              
HaeII        1493  AGCGCT               [GA]GCGC^[CT]
             4658  GGCGCC              
HgaI         4732  GACGC                GACGC(5/10)
             4894  GCGTC               
HincII       2497  GTCAAC               GT[CT]^[GA]AC
             4570  GTCAAC              
HindII       2497  GTCAAC               GT[CT]^[GA]AC
             4570  GTCAAC              
KpnI         2278  GGTACC               GGTAC^C
             2414  GGTACC              
MflI         2865  AGATCC               [GA]^GATC[CT]
             3442  AGATCT              
PflFI        3361  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3831  GACAGGGTC           
PinAI         162  ACCGGT               A^CCGGT
             2795  ACCGGT              
Tth111I      3361  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3831  GACAGGGTC           
XbaI         1137  TCTAGA               T^CTAGA
             2404  TCTAGA              
XhoII        2865  AGATCC               [GA]^GATC[CT]
             3442  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       AocI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SmaI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI