##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccII AccIII AclI Alw44I
ApaLI AscI AseI AsnI BamHI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
BstBI BstUI ClaI DraI DraIII
EagI EclXI Eco52I EcoRI FseI
HpaI KspI MamI MluI MroI
MvnI NaeI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SpeI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AhdI 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1493 AGCGCT AGC^GCT
Asp700I 1681 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3596 TGCGCA TGC^GCA
BbeI 4658 GGCGCC GGCGC^C
BbrPI 5035 CACGTG CAC^GTG
BcgI 4524 CGACAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2660 GGATAC GTATCC(6/5)
BglII 3442 AGATCT A^GATCT
BsiEI 2797 CGGTCG CG[GA][CT]^CG
BsmBI 1006 GAGACG CGTCTC(1/5)
BspHI 250 TCATGA T^CATGA
BsrBI 4102 GAGCGG CCGCTC(-3/-3)
Bst1107I 908 GTATAC GTA^TAC
BstEII 4041 GGTTACC G^GT[ACGT]ACC
BstPI 4041 GGTTACC G^GT[ACGT]ACC
BstZ17I 908 GTATAC GTA^TAC
CpoI 4628 CGGACCG CG^G[AT]CCG
DrdI 943 GACACAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 738 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1493 AGCGCT AGC^GCT
EcoO65I 4041 GGTTACC G^GT[ACGT]ACC
EcoRV 148 GATATC GAT^ATC
EcoT22I 1754 ATGCAT ATGCA^T
FspI 3596 TGCGCA TGC^GCA
HindIII 120 AAGCTT A^AGCTT
KasI 4658 GGCGCC G^GCGCC
MfeI 4479 CAATTG C^AATTG
MunI 4479 CAATTG C^AATTG
NarI 4658 GGCGCC GG^CGCC
NcoI 266 CCATGG C^CATGG
NheI 228 GCTAGC G^CTAGC
NsiI 1754 ATGCAT ATGCA^T
PmaCI 5035 CACGTG CAC^GTG
PmlI 5035 CACGTG CAC^GTG
RcaI 250 TCATGA T^CATGA
RsrII 4628 CGGACCG CG^G[AT]CCG
SapI 1978 GAAGAGC GCTCTTC(1/4)
SfoI 4658 GGCGCC GGC^GCC
SnaBI 978 TACGTA TAC^GTA
SspI 2563 AATATT AAT^ATT
XmnI 1681 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2278 GGTACC G^GTACC
2414 GGTACC
AcsI 986 AAATTT [GA]^AATT[CT]
2944 GAATTT
AflII 1380 CTTAAG C^TTAAG
3052 CTTAAG
AgeI 162 ACCGGT A^CCGGT
2795 ACCGGT
AlwI 2866 GATCC GGATC(4/5)
5107 GATCC
ApaI 2574 GGGCCC GGGCC^C
4798 GGGCCC
ApoI 986 AAATTT [GA]^AATT[CT]
2944 GAATTT
Asp718I 2278 GGTACC G^GTACC
2414 GGTACC
AspI 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3831 GACAGGGTC
AvrII 5084 CCTAGG C^CTAGG
5363 CCTAGG
BclI 3347 TGATCA T^GATCA
4781 TGATCA
BfrI 1380 CTTAAG C^TTAAG
3052 CTTAAG
BlnI 5084 CCTAGG C^CTAGG
5363 CCTAGG
BlpI 3264 GCTTAGC GC^T[ACGT]AGC
4014 GCTGAGC
Bpu1102I 3264 GCTTAGC GC^T[ACGT]AGC
4014 GCTGAGC
BspLU11I 767 ACATGT A^CATGT
777 ACATGT
BsrDI 3153 CATTGC GCAATG(2/0)
382 GCAATG
BsrFI 162 ACCGGT [GA]^CCGG[CT]
2795 ACCGGT
BstYI 2865 AGATCC [GA]^GATC[CT]
3442 AGATCT
CelII 3264 GCTTAGC GC^T[ACGT]AGC
4014 GCTGAGC
Cfr10I 162 ACCGGT [GA]^CCGG[CT]
2795 ACCGGT
FbaI 3347 TGATCA T^GATCA
4781 TGATCA
HaeII 1493 AGCGCT [GA]GCGC^[CT]
4658 GGCGCC
HgaI 4732 GACGC GACGC(5/10)
4894 GCGTC
HincII 2497 GTCAAC GT[CT]^[GA]AC
4570 GTCAAC
HindII 2497 GTCAAC GT[CT]^[GA]AC
4570 GTCAAC
KpnI 2278 GGTACC GGTAC^C
2414 GGTACC
MflI 2865 AGATCC [GA]^GATC[CT]
3442 AGATCT
PflFI 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3831 GACAGGGTC
PinAI 162 ACCGGT A^CCGGT
2795 ACCGGT
Tth111I 3361 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3831 GACAGGGTC
XbaI 1137 TCTAGA T^CTAGA
2404 TCTAGA
XhoII 2865 AGATCC [GA]^GATC[CT]
3442 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI