##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AccIII AgeI Asp718I
AvrII BciVI BglII BlnI BsaWI
BseAI BsiWI BsmI BspEI BspLU11I
Bst1107I BstAPI BstEII BstPI BstZ17I
CpoI DrdI EcoO65I EcoRI EcoRV
HindIII HpaI KpnI MluI MroI
NheI NruI PacI PinAI PmeI
PvuI RsrII SacI SapI SbfI
ScaI SexAI SfiI SgrAI Sse8387I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 4780 AACGTT AA^CGTT
AflII 844 CTTAAG C^TTAAG
AflIII 3826 ACGTGT A^C[GA][CT]GT
AhdI 2880 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2058 GTGCAC G^TGCAC
Aor51HI 3630 AGCGCT AGC^GCT
ApaLI 2058 GTGCAC G^TGCAC
AscI 56 GGCGCGCC GG^CGCGCC
AspEI 2880 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4786 TGCGCA TGC^GCA
BbrPI 1824 CACGTG CAC^GTG
BcgI 4235 CGAGAGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 844 CTTAAG C^TTAAG
BsaBI 980 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 2050 ATCGAT AT^CGAT
BssHII 57 GCGCGC G^CGCGC
BstBI 1487 TTCGAA TT^CGAA
ClaI 2050 ATCGAT AT^CGAT
DraIII 5026 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2880 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3630 AGCGCT AGC^GCT
EcoNI 1349 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5257 ATGCAT ATGCA^T
FseI 467 GGCCGGCC GGCCGG^CC
FspI 4786 TGCGCA TGC^GCA
MamI 980 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1661 CAATTG C^AATTG
MunI 1661 CAATTG C^AATTG
NdeI 1652 CATATG CA^TATG
NotI 328 GCGGCCGC GC^GGCCGC
NsiI 5257 ATGCAT ATGCA^T
NspV 1487 TTCGAA TT^CGAA
PaeR7I 2516 CTCGAG C^TCGAG
PflMI 1333 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1824 CACGTG CAC^GTG
PmlI 1824 CACGTG CAC^GTG
PpuMI 2087 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 4780 AACGTT AA^CGTT
PstI 2657 CTGCAG CTGCA^G
SalI 1012 GTCGAC G^TCGAC
SfuI 1487 TTCGAA TT^CGAA
SnaBI 4477 TACGTA TAC^GTA
SpeI 669 ACTAGT A^CTAGT
SspI 4732 AATATT AAT^ATT
StuI 5389 AGGCCT AGG^CCT
SwaI 4328 ATTTAAAT ATTT^AAAT
Van91I 1333 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3746 TCTAGA T^CTAGA
XhoI 2516 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 1411 CCTGAGG CC^T[ACGT]AGG
2836 CCTGAGG
ApaI 476 GGGCCC GGGCC^C
5321 GGGCCC
AseI 3663 ATTAAT AT^TAAT
3951 ATTAAT
AsnI 3663 ATTAAT AT^TAAT
3951 ATTAAT
Asp700I 1929 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2757 GAAGCAGTTC
AspI 1063 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3937 GACATTGTC
BamHI 1645 GGATCC G^GATCC
2286 GGATCC
BclI 2354 TGATCA T^GATCA
2969 TGATCA
BglI 2396 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4183 GCCTGCTCGGC
BsaI 1507 GAGACC GGTCTC(1/5)
2248 GAGACC
BsmBI 143 CGTCTC CGTCTC(1/5)
3430 GAGACG
BspMI 1438 ACCTGC ACCTGC(4/8)
570 GCAGGT
BsrBI 124 CCGCTC CCGCTC(-3/-3)
3346 CCGCTC
Bsu36I 1411 CCTGAGG CC^T[ACGT]AGG
2836 CCTGAGG
Eco81I 1411 CCTGAGG CC^T[ACGT]AGG
2836 CCTGAGG
FbaI 2354 TGATCA T^GATCA
2969 TGATCA
HincII 541 GTCAAC GT[CT]^[GA]AC
1012 GTCGAC
HindII 541 GTCAAC GT[CT]^[GA]AC
1012 GTCGAC
NcoI 488 CCATGG C^CATGG
2642 CCATGG
PflFI 1063 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3937 GACATTGTC
PshBI 3663 ATTAAT AT^TAAT
3951 ATTAAT
SmaI 161 CCCGGG CCC^GGG
389 CCCGGG
Tth111I 1063 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3937 GACATTGTC
XcmI 788 CCAATGCAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2064 CCACATCCGAGTTGG
XmaCI 161 CCCGGG C^CCGGG
389 CCCGGG
XmaI 161 CCCGGG C^CCGGG
389 CCCGGG
XmnI 1929 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2757 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PshAI PvuII
RcaI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII