##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BclI BfrI
BlnI BsaBI BsiEI BsiWI BspDI
BspHI BsrFI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV FbaI FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NspV PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 3261 GAATTC [GA]^AATT[CT]
Alw44I 1393 GTGCAC G^TGCAC
Aor51HI 677 AGCGCT AGC^GCT
ApaLI 1393 GTGCAC G^TGCAC
ApoI 3261 GAATTC [GA]^AATT[CT]
Asp700I 4703 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 347 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2598 TGGCCA TGG^CCA
BbeI 1208 GGCGCC GGCGC^C
BbrPI 3027 CACGTG CAC^GTG
BcgI 404 GCACCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4978 AGATCT A^GATCT
BsrDI 4132 GCAATG GCAATG(2/0)
DrdI 4555 GACAGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2598 TGGCCA [CT]^GGCC[GA]
Eco47III 677 AGCGCT AGC^GCT
EcoNI 1034 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3261 GAATTC G^AATTC
HindIII 2921 AAGCTT A^AGCTT
KasI 1208 GGCGCC G^GCGCC
MluNI 2598 TGGCCA TGG^CCA
MscI 2598 TGGCCA TGG^CCA
NarI 1208 GGCGCC GG^CGCC
NdeI 3067 CATATG CA^TATG
PacI 3330 TTAATTAA TTAAT^TAA
PflFI 347 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3027 CACGTG CAC^GTG
PmlI 3027 CACGTG CAC^GTG
SalI 2630 GTCGAC G^TCGAC
ScaI 4670 AGTACT AGT^ACT
SexAI 1039 ACCAGGT A^CC[AT]GGT
SfoI 1208 GGCGCC GGC^GCC
SphI 2523 GCATGC GCATG^C
SspI 5101 AATATT AAT^ATT
Tth111I 347 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3657 TCTAGA T^CTAGA
XmnI 4703 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1721 TCCGGA T^CCGGA
4551 TCCGGA
AcyI 1208 GGCGCC G[GA]^CG[CT]C
4808 GGCGTC
AocI 138 CCTCAGG CC^T[ACGT]AGG
4529 CCTGAGG
BciVI 127 GTATCC GTATCC(6/5)
2894 GTATCC
BlpI 2170 GCTGAGC GC^T[ACGT]AGC
4271 GCTGAGC
Bpu1102I 2170 GCTGAGC GC^T[ACGT]AGC
4271 GCTGAGC
BsaAI 3027 CACGTG [CT]AC^GT[GA]
4765 TACGTG
BsaHI 1208 GGCGCC G[GA]^CG[CT]C
4808 GGCGTC
BsaWI 1721 TCCGGA [AT]^CCGG[AT]
4551 TCCGGA
BseAI 1721 TCCGGA T^CCGGA
4551 TCCGGA
BsmBI 118 CGTCTC CGTCTC(1/5)
4929 CGTCTC
BsmI 839 GAATGC GAATGC(1/-1)
2247 GCATTC
Bsp1407I 1838 TGTACA T^GTACA
3640 TGTACA
BspEI 1721 TCCGGA T^CCGGA
4551 TCCGGA
BsrGI 1838 TGTACA T^GTACA
3640 TGTACA
BssHII 1571 GCGCGC G^CGCGC
3010 GCGCGC
Bsu36I 138 CCTCAGG CC^T[ACGT]AGG
4529 CCTGAGG
CelII 2170 GCTGAGC GC^T[ACGT]AGC
4271 GCTGAGC
DraI 2786 TTTAAA TTT^AAA
3098 TTTAAA
DraIII 1038 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3251 CACAAAGTG
DsaI 1991 CCATGG C^C[GA][CT]GG
2555 CCATGG
Eco81I 138 CCTCAGG CC^T[ACGT]AGG
4529 CCTGAGG
HgaI 666 GCGTC GACGC(5/10)
4809 GCGTC
Hin1I 1208 GGCGCC G[GA]^CG[CT]C
4808 GGCGTC
MroI 1721 TCCGGA T^CCGGA
4551 TCCGGA
NcoI 1991 CCATGG C^CATGG
2555 CCATGG
NheI 4154 GCTAGC G^CTAGC
4158 GCTAGC
SfiI 510 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
609 GGCCCTCTGGGCC
SmlI 2948 CTTGAG C^T[CT][GA]AG
4125 CTTGAG
SspBI 1838 TGTACA T^GTACA
3640 TGTACA
StuI 3993 AGGCCT AGG^CCT
4220 AGGCCT
TaqI 1657 TCGA T^CGA
2631 TCGA
TthHB8I 1657 TCGA T^CGA
2631 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI