################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AccIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco52I FauI FseI FspI KpnI KspI MamI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfuI SgrAI SmaI SnaBI SpeI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 4449 CGCG CG^CG Alw44I 2262 GTGCAC G^TGCAC Aor51HI 4916 AGCGCT AGC^GCT ApaLI 2262 GTGCAC G^TGCAC AvaI 1820 CCCGAG C^[CT]CG[GA]G BbeI 4004 GGCGCC GGCGC^C BcgI 2725 GCAGCCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2197 AGATCT A^GATCT BsaI 4828 GGTCTC GGTCTC(1/5) BsmBI 3568 GAGACG CGTCTC(1/5) BsoBI 1820 CCCGAG C^[CT]CG[GA]G BssSI 3420 CACGAG CACGAG(-5/-1) BstEII 6559 GGTAACC G^GT[ACGT]ACC BstPI 6559 GGTAACC G^GT[ACGT]ACC BstUI 4449 CGCG CG^CG DraIII 1995 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4916 AGCGCT AGC^GCT EcoNI 6096 CCTGTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 6559 GGTAACC G^GT[ACGT]ACC EcoRI 2994 GAATTC G^AATTC EcoRV 121 GATATC GAT^ATC HincII 1445 GTTAAC GT[CT]^[GA]AC HindII 1445 GTTAAC GT[CT]^[GA]AC HpaI 1445 GTTAAC GTT^AAC KasI 4004 GGCGCC G^GCGCC MfeI 1925 CAATTG C^AATTG MunI 1925 CAATTG C^AATTG MvnI 4449 CGCG CG^CG NarI 4004 GGCGCC GG^CGCC PshAI 1923 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 4836 GAGCTC GAGCT^C SapI 2670 GCTCTTC GCTCTTC(1/4) SexAI 387 ACCAGGT A^CC[AT]GGT SfoI 4004 GGCGCC GGC^GCC XcmI 5468 CCACGCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AclI 3167 AACGTT AA^CGTT 5721 AACGTT AflII 257 CTTAAG C^TTAAG 6422 CTTAAG AocI 192 CCTCAGG CC^T[ACGT]AGG 4980 CCTGAGG ApaI 1650 GGGCCC GGGCC^C 5399 GGGCCC BbrPI 812 CACGTG CAC^GTG 2696 CACGTG BbsI 997 GTCTTC GAAGAC(2/6) 3400 GTCTTC BciVI 1732 GTATCC GTATCC(6/5) 2098 GTATCC BfrI 257 CTTAAG C^TTAAG 6422 CTTAAG BglI 1294 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1685 GCCTATATGGC BpuAI 997 GTCTTC GAAGAC(2/6) 3400 GTCTTC BspHI 72 TCATGA T^CATGA 4390 TCATGA BspLU11I 2010 ACATGT A^CATGT 2767 ACATGT BstXI 518 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2529 CCAATTCTGTGG Bsu36I 192 CCTCAGG CC^T[ACGT]AGG 4980 CCTGAGG Eco81I 192 CCTCAGG CC^T[ACGT]AGG 4980 CCTGAGG EcoT22I 687 ATGCAT ATGCA^T 3645 ATGCAT HaeII 4004 GGCGCC [GA]GCGC^[CT] 4916 AGCGCT HindIII 5199 AAGCTT A^AGCTT 5353 AAGCTT NdeI 5212 CATATG CA^TATG 6574 CATATG NsiI 687 ATGCAT ATGCA^T 3645 ATGCAT PmaCI 812 CACGTG CAC^GTG 2696 CACGTG PmlI 812 CACGTG CAC^GTG 2696 CACGTG Psp1406I 3167 AACGTT AA^CGTT 5721 AACGTT RcaI 72 TCATGA T^CATGA 4390 TCATGA SbfI 1698 CCTGCAGG CCTGCA^GG 5018 CCTGCAGG SphI 685 GCATGC GCATG^C 1026 GCATGC Sse8387I 1698 CCTGCAGG CCTGCA^GG 5018 CCTGCAGG StuI 1683 AGGCCT AGG^CCT 3363 AGGCCT XbaI 2194 TCTAGA T^CTAGA 3901 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbvCI BbvI BclI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI