##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco52I FauI FseI FspI KpnI
KspI MamI MluI MroI NaeI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 4449 CGCG CG^CG
Alw44I 2262 GTGCAC G^TGCAC
Aor51HI 4916 AGCGCT AGC^GCT
ApaLI 2262 GTGCAC G^TGCAC
AvaI 1820 CCCGAG C^[CT]CG[GA]G
BbeI 4004 GGCGCC GGCGC^C
BcgI 2725 GCAGCCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2197 AGATCT A^GATCT
BsaI 4828 GGTCTC GGTCTC(1/5)
BsmBI 3568 GAGACG CGTCTC(1/5)
BsoBI 1820 CCCGAG C^[CT]CG[GA]G
BssSI 3420 CACGAG CACGAG(-5/-1)
BstEII 6559 GGTAACC G^GT[ACGT]ACC
BstPI 6559 GGTAACC G^GT[ACGT]ACC
BstUI 4449 CGCG CG^CG
DraIII 1995 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4916 AGCGCT AGC^GCT
EcoNI 6096 CCTGTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 6559 GGTAACC G^GT[ACGT]ACC
EcoRI 2994 GAATTC G^AATTC
EcoRV 121 GATATC GAT^ATC
HincII 1445 GTTAAC GT[CT]^[GA]AC
HindII 1445 GTTAAC GT[CT]^[GA]AC
HpaI 1445 GTTAAC GTT^AAC
KasI 4004 GGCGCC G^GCGCC
MfeI 1925 CAATTG C^AATTG
MunI 1925 CAATTG C^AATTG
MvnI 4449 CGCG CG^CG
NarI 4004 GGCGCC GG^CGCC
PshAI 1923 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4836 GAGCTC GAGCT^C
SapI 2670 GCTCTTC GCTCTTC(1/4)
SexAI 387 ACCAGGT A^CC[AT]GGT
SfoI 4004 GGCGCC GGC^GCC
XcmI 5468 CCACGCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 3167 AACGTT AA^CGTT
5721 AACGTT
AflII 257 CTTAAG C^TTAAG
6422 CTTAAG
AocI 192 CCTCAGG CC^T[ACGT]AGG
4980 CCTGAGG
ApaI 1650 GGGCCC GGGCC^C
5399 GGGCCC
BbrPI 812 CACGTG CAC^GTG
2696 CACGTG
BbsI 997 GTCTTC GAAGAC(2/6)
3400 GTCTTC
BciVI 1732 GTATCC GTATCC(6/5)
2098 GTATCC
BfrI 257 CTTAAG C^TTAAG
6422 CTTAAG
BglI 1294 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1685 GCCTATATGGC
BpuAI 997 GTCTTC GAAGAC(2/6)
3400 GTCTTC
BspHI 72 TCATGA T^CATGA
4390 TCATGA
BspLU11I 2010 ACATGT A^CATGT
2767 ACATGT
BstXI 518 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2529 CCAATTCTGTGG
Bsu36I 192 CCTCAGG CC^T[ACGT]AGG
4980 CCTGAGG
Eco81I 192 CCTCAGG CC^T[ACGT]AGG
4980 CCTGAGG
EcoT22I 687 ATGCAT ATGCA^T
3645 ATGCAT
HaeII 4004 GGCGCC [GA]GCGC^[CT]
4916 AGCGCT
HindIII 5199 AAGCTT A^AGCTT
5353 AAGCTT
NdeI 5212 CATATG CA^TATG
6574 CATATG
NsiI 687 ATGCAT ATGCA^T
3645 ATGCAT
PmaCI 812 CACGTG CAC^GTG
2696 CACGTG
PmlI 812 CACGTG CAC^GTG
2696 CACGTG
Psp1406I 3167 AACGTT AA^CGTT
5721 AACGTT
RcaI 72 TCATGA T^CATGA
4390 TCATGA
SbfI 1698 CCTGCAGG CCTGCA^GG
5018 CCTGCAGG
SphI 685 GCATGC GCATG^C
1026 GCATGC
Sse8387I 1698 CCTGCAGG CCTGCA^GG
5018 CCTGCAGG
StuI 1683 AGGCCT AGG^CCT
3363 AGGCCT
XbaI 2194 TCTAGA T^CTAGA
3901 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI