################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AcsI AhdI AocI ApoI AscI AseI AsnI AspEI AvrII BamHI BbvCI BlnI BseAI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI HpaI KspI MfeI MluI MroI MunI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 82 GGTACC G^GTACC AccI 2180 GTCTAC GT^[AC][GT]AC AflII 1586 CTTAAG C^TTAAG AflIII 4185 ACATGT A^C[GA][CT]GT Alw44I 491 GTGCAC G^TGCAC ApaLI 491 GTGCAC G^TGCAC Asp718I 82 GGTACC G^GTACC AviII 2562 TGCGCA TGC^GCA BcgI 2646 GCAGTGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1586 CTTAAG C^TTAAG BglI 5036 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 996 AGATCT A^GATCT BlpI 134 GCTCAGC GC^T[ACGT]AGC Bpu1102I 134 GCTCAGC GC^T[ACGT]AGC BsaBI 1426 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 4529 GAATGC GAATGC(1/-1) BspHI 981 TCATGA T^CATGA BspLU11I 4185 ACATGT A^CATGT BsrBI 1475 GAGCGG CCGCTC(-3/-3) BstAPI 2809 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 134 GCTCAGC GC^T[ACGT]AGC DraIII 1238 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2171 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 2562 TGCGCA TGC^GCA HindIII 3952 AAGCTT A^AGCTT KpnI 82 GGTACC GGTAC^C MamI 1426 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 1647 CATATG CA^TATG NheI 2642 GCTAGC G^CTAGC PaeR7I 4833 CTCGAG C^TCGAG PvuI 1425 CGATCG CGAT^CG RcaI 981 TCATGA T^CATGA SbfI 3529 CCTGCAGG CCTGCA^GG ScaI 2811 AGTACT AGT^ACT SexAI 4993 ACCTGGT A^CC[AT]GGT SmaI 4467 CCCGGG CCC^GGG SphI 1204 GCATGC GCATG^C Sse8387I 3529 CCTGCAGG CCTGCA^GG XhoI 4833 CTCGAG C^TCGAG XmaCI 4467 CCCGGG C^CCGGG XmaI 4467 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AgeI 487 ACCGGT A^CCGGT 4257 ACCGGT ApaI 361 GGGCCC GGGCC^C 2255 GGGCCC Asp700I 2594 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3802 GAAGCTCTTC AspI 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1526 GACCGTGTC BalI 3834 TGGCCA TGG^CCA 4953 TGGCCA BbeI 336 GGCGCC GGCGC^C 5162 GGCGCC BbrPI 800 CACGTG CAC^GTG 3376 CACGTG BciVI 2093 GGATAC GTATCC(6/5) 4427 GTATCC BclI 939 TGATCA T^GATCA 1401 TGATCA BsiEI 1425 CGATCG CG[GA][CT]^CG 1525 CGACCG BsmBI 269 CGTCTC CGTCTC(1/5) 1006 CGTCTC Bsp1407I 1902 TGTACA T^GTACA 4740 TGTACA BsrGI 1902 TGTACA T^GTACA 4740 TGTACA BstXI 2280 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3567 CCATTGTCCTGG FbaI 939 TGATCA T^GATCA 1401 TGATCA KasI 336 GGCGCC G^GCGCC 5162 GGCGCC MluNI 3834 TGGCCA TGG^CCA 4953 TGGCCA MscI 3834 TGGCCA TGG^CCA 4953 TGGCCA NaeI 2737 GCCGGC GCC^GGC 3701 GCCGGC NarI 336 GGCGCC GG^CGCC 5162 GGCGCC NcoI 2001 CCATGG C^CATGG 3454 CCATGG NgoMIV 2737 GCCGGC G^CCGGC 3701 GCCGGC PflFI 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1526 GACCGTGTC PinAI 487 ACCGGT A^CCGGT 4257 ACCGGT PmaCI 800 CACGTG CAC^GTG 3376 CACGTG PmlI 800 CACGTG CAC^GTG 3376 CACGTG SacI 985 GAGCTC GAGCT^C 2325 GAGCTC SfoI 336 GGCGCC GGC^GCC 5162 GGCGCC SspBI 1902 TGTACA T^GTACA 4740 TGTACA TfiI 1817 GATTC G^A[AT]TC 3241 GATTC Tsp509I 2966 AATT ^AATT 5268 AATT Tth111I 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1526 GACCGTGTC XmnI 2594 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3802 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII