##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AcsI AhdI
AocI ApoI AscI AseI AsnI
AspEI AvrII BamHI BbvCI BlnI
BseAI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI HpaI KspI MfeI
MluI MroI MunI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I RsrII SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 82 GGTACC G^GTACC
AccI 2180 GTCTAC GT^[AC][GT]AC
AflII 1586 CTTAAG C^TTAAG
AflIII 4185 ACATGT A^C[GA][CT]GT
Alw44I 491 GTGCAC G^TGCAC
ApaLI 491 GTGCAC G^TGCAC
Asp718I 82 GGTACC G^GTACC
AviII 2562 TGCGCA TGC^GCA
BcgI 2646 GCAGTGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1586 CTTAAG C^TTAAG
BglI 5036 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 996 AGATCT A^GATCT
BlpI 134 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 134 GCTCAGC GC^T[ACGT]AGC
BsaBI 1426 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4529 GAATGC GAATGC(1/-1)
BspHI 981 TCATGA T^CATGA
BspLU11I 4185 ACATGT A^CATGT
BsrBI 1475 GAGCGG CCGCTC(-3/-3)
BstAPI 2809 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 134 GCTCAGC GC^T[ACGT]AGC
DraIII 1238 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2171 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2562 TGCGCA TGC^GCA
HindIII 3952 AAGCTT A^AGCTT
KpnI 82 GGTACC GGTAC^C
MamI 1426 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1647 CATATG CA^TATG
NheI 2642 GCTAGC G^CTAGC
PaeR7I 4833 CTCGAG C^TCGAG
PvuI 1425 CGATCG CGAT^CG
RcaI 981 TCATGA T^CATGA
SbfI 3529 CCTGCAGG CCTGCA^GG
ScaI 2811 AGTACT AGT^ACT
SexAI 4993 ACCTGGT A^CC[AT]GGT
SmaI 4467 CCCGGG CCC^GGG
SphI 1204 GCATGC GCATG^C
Sse8387I 3529 CCTGCAGG CCTGCA^GG
XhoI 4833 CTCGAG C^TCGAG
XmaCI 4467 CCCGGG C^CCGGG
XmaI 4467 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AgeI 487 ACCGGT A^CCGGT
4257 ACCGGT
ApaI 361 GGGCCC GGGCC^C
2255 GGGCCC
Asp700I 2594 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3802 GAAGCTCTTC
AspI 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACCGTGTC
BalI 3834 TGGCCA TGG^CCA
4953 TGGCCA
BbeI 336 GGCGCC GGCGC^C
5162 GGCGCC
BbrPI 800 CACGTG CAC^GTG
3376 CACGTG
BciVI 2093 GGATAC GTATCC(6/5)
4427 GTATCC
BclI 939 TGATCA T^GATCA
1401 TGATCA
BsiEI 1425 CGATCG CG[GA][CT]^CG
1525 CGACCG
BsmBI 269 CGTCTC CGTCTC(1/5)
1006 CGTCTC
Bsp1407I 1902 TGTACA T^GTACA
4740 TGTACA
BsrGI 1902 TGTACA T^GTACA
4740 TGTACA
BstXI 2280 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3567 CCATTGTCCTGG
FbaI 939 TGATCA T^GATCA
1401 TGATCA
KasI 336 GGCGCC G^GCGCC
5162 GGCGCC
MluNI 3834 TGGCCA TGG^CCA
4953 TGGCCA
MscI 3834 TGGCCA TGG^CCA
4953 TGGCCA
NaeI 2737 GCCGGC GCC^GGC
3701 GCCGGC
NarI 336 GGCGCC GG^CGCC
5162 GGCGCC
NcoI 2001 CCATGG C^CATGG
3454 CCATGG
NgoMIV 2737 GCCGGC G^CCGGC
3701 GCCGGC
PflFI 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACCGTGTC
PinAI 487 ACCGGT A^CCGGT
4257 ACCGGT
PmaCI 800 CACGTG CAC^GTG
3376 CACGTG
PmlI 800 CACGTG CAC^GTG
3376 CACGTG
SacI 985 GAGCTC GAGCT^C
2325 GAGCTC
SfoI 336 GGCGCC GGC^GCC
5162 GGCGCC
SspBI 1902 TGTACA T^GTACA
4740 TGTACA
TfiI 1817 GATTC G^A[AT]TC
3241 GATTC
Tsp509I 2966 AATT ^AATT
5268 AATT
Tth111I 1508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACCGTGTC
XmnI 2594 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3802 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII