##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AgeI Aor51HI AscI
Asp718I AviII AvrII BciVI BglI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BssHII BstBI BstEII BstPI CelII
ClaI CpoI Eco47III EcoO65I FseI
FspI HindIII KpnI MluI MroI
NaeI NgoMIV NotI NruI NspV
PacI PinAI PmeI PpuMI PshAI
PvuI PvuII RcaI RsrII SalI
SapI SfiI SfuI SgrAI SmaI
SnaBI SpeI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 997 GACGTC GACGT^C
AhdI 758 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1715 GGGCCC GGGCC^C
Asp700I 2759 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 758 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1663 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 734 CTCGAG C^[CT]CG[GA]G
BbeI 223 GGCGCC GGCGC^C
BbvCI 2733 GCTGAGG CCTCAGC(-5/-2)
BcgI 4160 CGAATTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6401 TGATCA T^GATCA
BglII 738 AGATCT A^GATCT
BsiEI 145 CGGCCG CG[GA][CT]^CG
BsoBI 734 CTCGAG C^[CT]CG[GA]G
BspLU11I 6384 ACATGT A^CATGT
BsrBI 130 CCGCTC CCGCTC(-3/-3)
BssSI 82 CTCGTG CACGAG(-5/-1)
Bst1107I 6001 GTATAC GTA^TAC
BstZ17I 6001 GTATAC GTA^TAC
DrdI 2064 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 145 CGGCCG C^GGCCG
Eam1105I 758 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 145 CGGCCG C^GGCCG
Eco52I 145 CGGCCG C^GGCCG
EcoRV 1415 GATATC GAT^ATC
FbaI 6401 TGATCA T^GATCA
HgaI 190 GCGTC GACGC(5/10)
KasI 223 GGCGCC G^GCGCC
KspI 46 CCGCGG CCGC^GG
MfeI 5023 CAATTG C^AATTG
MspA1I 46 CCGCGG C[AC]G^C[GT]G
MunI 5023 CAATTG C^AATTG
NarI 223 GGCGCC GG^CGCC
PaeR7I 734 CTCGAG C^TCGAG
PflFI 1663 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 46 CCGCGG CCGC^GG
SbfI 4836 CCTGCAGG CCTGCA^GG
SfoI 223 GGCGCC GGC^GCC
Sse8387I 4836 CCTGCAGG CCTGCA^GG
StuI 3019 AGGCCT AGG^CCT
SwaI 6147 ATTTAAAT ATTT^AAAT
Tth111I 1663 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 734 CTCGAG C^TCGAG
XmnI 2759 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AclI 1194 AACGTT AA^CGTT
2413 AACGTT
AcyI 223 GGCGCC G[GA]^CG[CT]C
997 GACGTC
AflIII 4637 ACACGT A^C[GA][CT]GT
6384 ACATGT
Alw44I 389 GTGCAC G^TGCAC
842 GTGCAC
ApaLI 389 GTGCAC G^TGCAC
842 GTGCAC
BanI 223 GGCGCC G^G[CT][GA]CC
1681 GGCACC
BbrPI 1645 CACGTG CAC^GTG
4638 CACGTG
BsaAI 1645 CACGTG [CT]AC^GT[GA]
4638 CACGTG
BsaBI 1563 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5794 GATGACCATC
BsaHI 223 GGCGCC G[GA]^CG[CT]C
997 GACGTC
BsaI 4284 GGTCTC GGTCTC(1/5)
4306 GGTCTC
BseRI 731 CTCCTC GAGGAG(10/8)
2042 GAGGAG
BstAPI 2159 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5563 GCAGTGTCTGC
BstXI 2031 CCAATCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3250 CCAGATCCCTGG
DraII 176 GGGGCCT [GA]G^G[ACGT]CC[CT]
691 GGGGCCT
DraIII 247 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
5691 CACACTGTG
EcoO109I 176 GGGGCCT [GA]G^G[ACGT]CC[CT]
691 GGGGCCT
EcoT22I 611 ATGCAT ATGCA^T
2611 ATGCAT
HaeII 103 GGCGCT [GA]GCGC^[CT]
223 GGCGCC
Hin1I 223 GGCGCC G[GA]^CG[CT]C
997 GACGTC
HpaI 5211 GTTAAC GTT^AAC
5483 GTTAAC
MamI 1563 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5794 GATGACCATC
NheI 2909 GCTAGC G^CTAGC
5632 GCTAGC
NsiI 611 ATGCAT ATGCA^T
2611 ATGCAT
PmaCI 1645 CACGTG CAC^GTG
4638 CACGTG
PmlI 1645 CACGTG CAC^GTG
4638 CACGTG
Psp1406I 1194 AACGTT AA^CGTT
2413 AACGTT
SacI 79 GAGCTC GAGCT^C
658 GAGCTC
SexAI 1634 ACCTGGT A^CC[AT]GGT
4140 ACCAGGT
SphI 1388 GCATGC GCATG^C
4054 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AseI AsnI AspHI AvaII
BalI BamHI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI Eco81I EcoNI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII