##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BclI BglI BlnI BsiEI BsiWI
BsoBI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FbaI
FseI FspI KpnI MluI NheI
NotI NruI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 3571 TCCGGA T^CCGGA
AflII 1643 CTTAAG C^TTAAG
AgeI 5402 ACCGGT A^CCGGT
AspI 959 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1455 GCAGCTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1643 CTTAAG C^TTAAG
BlpI 2562 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2562 GCTAAGC GC^T[ACGT]AGC
BseAI 3571 TCCGGA T^CCGGA
Bsp1407I 511 TGTACA T^GTACA
BspEI 3571 TCCGGA T^CCGGA
BspLU11I 514 ACATGT A^CATGT
BsrDI 939 CATTGC GCAATG(2/0)
BsrGI 511 TGTACA T^GTACA
BssSI 5763 CTCGTG CACGAG(-5/-1)
BstEII 5405 GGTTACC G^GT[ACGT]ACC
BstPI 5405 GGTTACC G^GT[ACGT]ACC
CelII 2562 GCTAAGC GC^T[ACGT]AGC
DraI 5671 TTTAAA TTT^AAA
EcoNI 5024 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5405 GGTTACC G^GT[ACGT]ACC
EcoRV 3660 GATATC GAT^ATC
EcoT22I 5771 ATGCAT ATGCA^T
HpaI 6334 GTTAAC GTT^AAC
MfeI 636 CAATTG C^AATTG
MroI 3571 TCCGGA T^CCGGA
MunI 636 CAATTG C^AATTG
NdeI 4348 CATATG CA^TATG
NsiI 5771 ATGCAT ATGCA^T
PflFI 959 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5402 ACCGGT A^CCGGT
PpuMI 3939 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2345 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5728 GAGCTC GAGCT^C
SexAI 4556 ACCAGGT A^CC[AT]GGT
SspBI 511 TGTACA T^GTACA
SspI 4116 AATATT AAT^ATT
Tth111I 959 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 425 TCTAGA T^CTAGA
XcmI 5708 CCAAGGCATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 1785 GACGTC GACGT^C
3188 GACGTC
AccI 360 GTCTAC GT^[AC][GT]AC
3498 GTCTAC
BamHI 213 GGATCC G^GATCC
252 GGATCC
BbeI 4230 GGCGCC GGCGC^C
5133 GGCGCC
BbrPI 3012 CACGTG CAC^GTG
5963 CACGTG
BbvCI 1324 CCTCAGC CCTCAGC(-5/-2)
5994 CCTCAGC
BcnI 85 CCGGG CC^[GC]GG
535 CCGGG
BspHI 2470 TCATGA T^CATGA
6294 TCATGA
BsrBI 4178 CCGCTC CCGCTC(-3/-3)
500 GAGCGG
DrdI 605 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5749 GACATCCTGGTC
HincII 4790 GTCAAC GT[CT]^[GA]AC
6334 GTTAAC
HindII 4790 GTCAAC GT[CT]^[GA]AC
6334 GTTAAC
KasI 4230 GGCGCC G^GCGCC
5133 GGCGCC
KspI 63 CCGCGG CCGC^GG
4605 CCGCGG
NaeI 2893 GCCGGC GCC^GGC
2940 GCCGGC
NarI 4230 GGCGCC GG^CGCC
5133 GGCGCC
NciI 85 CCGGG CC^[GC]GG
535 CCGGG
NgoMIV 2893 GCCGGC G^CCGGC
2940 GCCGGC
PmaCI 3012 CACGTG CAC^GTG
5963 CACGTG
PmlI 3012 CACGTG CAC^GTG
5963 CACGTG
RcaI 2470 TCATGA T^CATGA
6294 TCATGA
SacII 63 CCGCGG CCGC^GG
4605 CCGCGG
SfoI 4230 GGCGCC GGC^GCC
5133 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI