##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AflII AgeI AscI Asp700I
AvrII BfrI BlnI BsiWI BsmBI
Bsp1407I BspHI BsrFI BsrGI BssHII
BstBI Cfr10I CpoI FseI KspI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NspV PacI PinAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2992 GACGTC GACGT^C
Acc65I 4749 GGTACC G^GTACC
AccIII 1993 TCCGGA T^CCGGA
Aor51HI 5691 AGCGCT AGC^GCT
AseI 5874 ATTAAT AT^TAAT
AsnI 5874 ATTAAT AT^TAAT
Asp718I 4749 GGTACC G^GTACC
BbeI 78 GGCGCC GGCGC^C
BcgI 1328 CGAGTTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 9 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3459 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3459 GCTGAGC GC^T[ACGT]AGC
BsaBI 1739 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1993 TCCGGA T^CCGGA
BsiEI 614 CGGCCG CG[GA][CT]^CG
BspDI 2586 ATCGAT AT^CGAT
BspEI 1993 TCCGGA T^CCGGA
BstEII 1809 GGTCACC G^GT[ACGT]ACC
BstPI 1809 GGTCACC G^GT[ACGT]ACC
BstXI 2653 CCAACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3459 GCTGAGC GC^T[ACGT]AGC
ClaI 2586 ATCGAT AT^CGAT
EagI 614 CGGCCG C^GGCCG
EclXI 614 CGGCCG C^GGCCG
Eco47III 5691 AGCGCT AGC^GCT
Eco52I 614 CGGCCG C^GGCCG
EcoO65I 1809 GGTCACC G^GT[ACGT]ACC
EcoRV 6745 GATATC GAT^ATC
EcoT22I 3105 ATGCAT ATGCA^T
KasI 78 GGCGCC G^GCGCC
KpnI 4749 GGTACC GGTAC^C
MamI 1739 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1993 TCCGGA T^CCGGA
NarI 78 GGCGCC GG^CGCC
NdeI 5151 CATATG CA^TATG
NheI 3870 GCTAGC G^CTAGC
NsiI 3105 ATGCAT ATGCA^T
PmeI 3383 GTTTAAAC GTTT^AAAC
PshBI 5874 ATTAAT AT^TAAT
SfoI 78 GGCGCC GGC^GCC
SmaI 2780 CCCGGG CCC^GGG
SphI 1648 GCATGC GCATG^C
XmaCI 2780 CCCGGG C^CCGGG
XmaI 2780 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 2610 CGCG CG^CG
3487 CGCG
AhdI 4909 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5749 GACCCAAGGTC
ApaI 2452 GGGCCC GGGCC^C
6191 GGGCCC
AspEI 4909 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5749 GACCCAAGGTC
AviII 1397 TGCGCA TGC^GCA
6600 TGCGCA
BalI 1536 TGGCCA TGG^CCA
6433 TGGCCA
BamHI 2106 GGATCC G^GATCC
2153 GGATCC
BbrPI 2645 CACGTG CAC^GTG
3329 CACGTG
BciVI 2463 GGATAC GTATCC(6/5)
4481 GGATAC
BclI 2402 TGATCA T^GATCA
5913 TGATCA
BsgI 6219 CTGCAC GTGCAG(16/14)
5320 GTGCAG
BspMI 3138 ACCTGC ACCTGC(4/8)
6522 ACCTGC
BsrBI 3223 CCGCTC CCGCTC(-3/-3)
2019 GAGCGG
BsrDI 2624 CATTGC GCAATG(2/0)
6481 GCAATG
BssSI 2718 CTCGTG CACGAG(-5/-1)
5985 CTCGTG
Bst1107I 1369 GTATAC GTA^TAC
4520 GTATAC
BstUI 2610 CGCG CG^CG
3487 CGCG
BstZ17I 1369 GTATAC GTA^TAC
4520 GTATAC
DraIII 3478 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4384 CACCATGTG
Eam1105I 4909 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5749 GACCCAAGGTC
EcoNI 225 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5561 CCTTGCCCAGG
EcoRI 3185 GAATTC G^AATTC
3589 GAATTC
FbaI 2402 TGATCA T^GATCA
5913 TGATCA
FspI 1397 TGCGCA TGC^GCA
6600 TGCGCA
HindIII 3554 AAGCTT A^AGCTT
5826 AAGCTT
HpaI 3412 GTTAAC GTT^AAC
4794 GTTAAC
MluNI 1536 TGGCCA TGG^CCA
6433 TGGCCA
MscI 1536 TGGCCA TGG^CCA
6433 TGGCCA
MvnI 2610 CGCG CG^CG
3487 CGCG
NcoI 4745 CCATGG C^CATGG
6312 CCATGG
PaeR7I 1501 CTCGAG C^TCGAG
2083 CTCGAG
PmaCI 2645 CACGTG CAC^GTG
3329 CACGTG
PmlI 2645 CACGTG CAC^GTG
3329 CACGTG
PshAI 2067 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5767 GACTTGAGTC
XbaI 3159 TCTAGA T^CTAGA
5498 TCTAGA
XhoI 1501 CTCGAG C^TCGAG
2083 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmlI SpeI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII