##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AflII Alw44I AocI
Aor51HI ApaI ApaLI AscI AspI
AviII AvrII BbrPI BcgI BciVI
BfrI BlnI BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI BspDI BssHII
BstEII BstPI Bsu36I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HpaI KspI MamI
MluI NdeI NheI NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 3321 CGCG CG^CG
AccIII 1574 TCCGGA T^CCGGA
AflIII 1337 ACATGT A^C[GA][CT]GT
AgeI 1391 ACCGGT A^CCGGT
AhdI 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4751 ATTAAT AT^TAAT
AsnI 4751 ATTAAT AT^TAAT
Asp700I 2563 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 472 GGATCC G^GATCC
BbeI 4722 GGCGCC GGCGC^C
BglII 761 AGATCT A^GATCT
BlpI 4236 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4236 GCTAAGC GC^T[ACGT]AGC
BseAI 1574 TCCGGA T^CCGGA
BspEI 1574 TCCGGA T^CCGGA
BspHI 426 TCATGA T^CATGA
BspLU11I 1337 ACATGT A^CATGT
BsrBI 1823 CCGCTC CCGCTC(-3/-3)
Bst1107I 2844 GTATAC GTA^TAC
BstAPI 2242 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 497 TTCGAA TT^CGAA
BstUI 3321 CGCG CG^CG
BstZ17I 2844 GTATAC GTA^TAC
CelII 4236 GCTAAGC GC^T[ACGT]AGC
DrdI 201 GACAGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 4722 GGCGCC [GA]GCGC^[CT]
HincII 2616 GTTGAC GT[CT]^[GA]AC
HindII 2616 GTTGAC GT[CT]^[GA]AC
KasI 4722 GGCGCC G^GCGCC
MfeI 2850 CAATTG C^AATTG
MroI 1574 TCCGGA T^CCGGA
MunI 2850 CAATTG C^AATTG
MvnI 3321 CGCG CG^CG
NarI 4722 GGCGCC GG^CGCC
NspV 497 TTCGAA TT^CGAA
PaeR7I 3676 CTCGAG C^TCGAG
PinAI 1391 ACCGGT A^CCGGT
PshBI 4751 ATTAAT AT^TAAT
RcaI 426 TCATGA T^CATGA
SfoI 4722 GGCGCC GGC^GCC
SfuI 497 TTCGAA TT^CGAA
SpeI 2838 ACTAGT A^CTAGT
SphI 1818 GCATGC GCATG^C
XhoI 3676 CTCGAG C^TCGAG
XmnI 2563 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1070 GGTACC G^GTACC
1515 GGTACC
AcyI 733 GGCGTC G[GA]^CG[CT]C
4722 GGCGCC
Asp718I 1070 GGTACC G^GTACC
1515 GGTACC
BglI 2409 GCCTAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2935 GCCCTCTCGGC
BsaHI 733 GGCGTC G[GA]^CG[CT]C
4722 GGCGCC
BsaWI 1391 ACCGGT [AT]^CCGG[AT]
1574 TCCGGA
BspMI 4204 ACCTGC ACCTGC(4/8)
3381 GCAGGT
BsrDI 1894 GCAATG GCAATG(2/0)
2107 GCAATG
BssSI 1754 CACGAG CACGAG(-5/-1)
853 CTCGTG
BstXI 2202 CCAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3546 CCAGACAGGTGG
EcoNI 716 CCTCTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1704 CCTATTCCAGG
EcoRI 281 GAATTC G^AATTC
661 GAATTC
HgaI 734 GCGTC GACGC(5/10)
2371 GCGTC
Hin1I 733 GGCGTC G[GA]^CG[CT]C
4722 GGCGCC
HindIII 2565 AAGCTT A^AGCTT
4598 AAGCTT
KpnI 1070 GGTACC GGTAC^C
1515 GGTACC
MslI 582 CATTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
595 CACACTCATG
NaeI 40 GCCGGC GCC^GGC
3927 GCCGGC
NgoMIV 40 GCCGGC G^CCGGC
3927 GCCGGC
PflMI 3288 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3767 CCAGTTTATGG
PshAI 66 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3712 GACCAGAGTC
SmaI 185 CCCGGG CCC^GGG
1505 CCCGGG
SmlI 2362 CTCAAG C^T[CT][GA]AG
3676 CTCGAG
StuI 129 AGGCCT AGG^CCT
4297 AGGCCT
Van91I 3288 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3767 CCAGTTTATGG
XmaCI 185 CCCGGG C^CCGGG
1505 CCCGGG
XmaI 185 CCCGGG C^CCGGG
1505 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII