################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AclI AflII Alw44I AocI Aor51HI ApaI ApaLI AscI AspI AviII AvrII BbrPI BcgI BciVI BfrI BlnI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspDI BssHII BstEII BstPI Bsu36I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HpaI KspI MamI MluI NdeI NheI NotI NruI NsiI PacI PflFI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 3321 CGCG CG^CG AccIII 1574 TCCGGA T^CCGGA AflIII 1337 ACATGT A^C[GA][CT]GT AgeI 1391 ACCGGT A^CCGGT AhdI 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 4751 ATTAAT AT^TAAT AsnI 4751 ATTAAT AT^TAAT Asp700I 2563 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 472 GGATCC G^GATCC BbeI 4722 GGCGCC GGCGC^C BglII 761 AGATCT A^GATCT BlpI 4236 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4236 GCTAAGC GC^T[ACGT]AGC BseAI 1574 TCCGGA T^CCGGA BspEI 1574 TCCGGA T^CCGGA BspHI 426 TCATGA T^CATGA BspLU11I 1337 ACATGT A^CATGT BsrBI 1823 CCGCTC CCGCTC(-3/-3) Bst1107I 2844 GTATAC GTA^TAC BstAPI 2242 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 497 TTCGAA TT^CGAA BstUI 3321 CGCG CG^CG BstZ17I 2844 GTATAC GTA^TAC CelII 4236 GCTAAGC GC^T[ACGT]AGC DrdI 201 GACAGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 4722 GGCGCC [GA]GCGC^[CT] HincII 2616 GTTGAC GT[CT]^[GA]AC HindII 2616 GTTGAC GT[CT]^[GA]AC KasI 4722 GGCGCC G^GCGCC MfeI 2850 CAATTG C^AATTG MroI 1574 TCCGGA T^CCGGA MunI 2850 CAATTG C^AATTG MvnI 3321 CGCG CG^CG NarI 4722 GGCGCC GG^CGCC NspV 497 TTCGAA TT^CGAA PaeR7I 3676 CTCGAG C^TCGAG PinAI 1391 ACCGGT A^CCGGT PshBI 4751 ATTAAT AT^TAAT RcaI 426 TCATGA T^CATGA SfoI 4722 GGCGCC GGC^GCC SfuI 497 TTCGAA TT^CGAA SpeI 2838 ACTAGT A^CTAGT SphI 1818 GCATGC GCATG^C XhoI 3676 CTCGAG C^TCGAG XmnI 2563 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 1070 GGTACC G^GTACC 1515 GGTACC AcyI 733 GGCGTC G[GA]^CG[CT]C 4722 GGCGCC Asp718I 1070 GGTACC G^GTACC 1515 GGTACC BglI 2409 GCCTAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2935 GCCCTCTCGGC BsaHI 733 GGCGTC G[GA]^CG[CT]C 4722 GGCGCC BsaWI 1391 ACCGGT [AT]^CCGG[AT] 1574 TCCGGA BspMI 4204 ACCTGC ACCTGC(4/8) 3381 GCAGGT BsrDI 1894 GCAATG GCAATG(2/0) 2107 GCAATG BssSI 1754 CACGAG CACGAG(-5/-1) 853 CTCGTG BstXI 2202 CCAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3546 CCAGACAGGTGG EcoNI 716 CCTCTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1704 CCTATTCCAGG EcoRI 281 GAATTC G^AATTC 661 GAATTC HgaI 734 GCGTC GACGC(5/10) 2371 GCGTC Hin1I 733 GGCGTC G[GA]^CG[CT]C 4722 GGCGCC HindIII 2565 AAGCTT A^AGCTT 4598 AAGCTT KpnI 1070 GGTACC GGTAC^C 1515 GGTACC MslI 582 CATTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 595 CACACTCATG NaeI 40 GCCGGC GCC^GGC 3927 GCCGGC NgoMIV 40 GCCGGC G^CCGGC 3927 GCCGGC PflMI 3288 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3767 CCAGTTTATGG PshAI 66 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3712 GACCAGAGTC SmaI 185 CCCGGG CCC^GGG 1505 CCCGGG SmlI 2362 CTCAAG C^T[CT][GA]AG 3676 CTCGAG StuI 129 AGGCCT AGG^CCT 4297 AGGCCT Van91I 3288 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3767 CCAGTTTATGG XmaCI 185 CCCGGG C^CCGGG 1505 CCCGGG XmaI 185 CCCGGG C^CCGGG 1505 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII