##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccII AclI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AvrII BbeI BbrPI BglI BlnI
BsaAI BsiWI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI KasI
KspI MfeI MluI MunI MvnI
NarI NotI NruI PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 4686 TCCGGA T^CCGGA
AflII 6242 CTTAAG C^TTAAG
AgeI 1369 ACCGGT A^CCGGT
Alw44I 1753 GTGCAC G^TGCAC
ApaLI 1753 GTGCAC G^TGCAC
AspI 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 238 TGCGCA TGC^GCA
BamHI 1588 GGATCC G^GATCC
BbvCI 1690 GCTGAGG CCTCAGC(-5/-2)
BcgI 4755 GCAAAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 125 GTATCC GTATCC(6/5)
BfrI 6242 CTTAAG C^TTAAG
BlpI 2983 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2983 GCTGAGC GC^T[ACGT]AGC
BseAI 4686 TCCGGA T^CCGGA
BsiEI 47 CGGTCG CG[GA][CT]^CG
BsmBI 5373 GAGACG CGTCTC(1/5)
BspDI 3869 ATCGAT AT^CGAT
BspEI 4686 TCCGGA T^CCGGA
BspHI 2499 TCATGA T^CATGA
BssSI 5867 CTCGTG CACGAG(-5/-1)
BstBI 322 TTCGAA TT^CGAA
CelII 2983 GCTGAGC GC^T[ACGT]AGC
ClaI 3869 ATCGAT AT^CGAT
DraIII 4019 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1272 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 238 TGCGCA TGC^GCA
HgaI 55 GCGTC GACGC(5/10)
HpaI 4027 GTTAAC GTT^AAC
MroI 4686 TCCGGA T^CCGGA
NaeI 3907 GCCGGC GCC^GGC
NdeI 1081 CATATG CA^TATG
NgoMIV 3907 GCCGGC G^CCGGC
NheI 1211 GCTAGC G^CTAGC
NspV 322 TTCGAA TT^CGAA
PaeR7I 4653 CTCGAG C^TCGAG
PflFI 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1369 ACCGGT A^CCGGT
PshAI 3238 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2499 TCATGA T^CATGA
SfuI 322 TTCGAA TT^CGAA
SmaI 1647 CCCGGG CCC^GGG
SphI 5818 GCATGC GCATG^C
SspI 5286 AATATT AAT^ATT
Tth111I 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4653 CTCGAG C^TCGAG
XmaCI 1647 CCCGGG C^CCGGG
XmaI 1647 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 3413 GTAGAC GT^[AC][GT]AC
5497 GTAGAC
AflIII 2101 ACATGT A^C[GA][CT]GT
3239 ACATGT
Asp700I 846 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2251 GAAAGCTTTC
BsaBI 3544 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5168 GATGTTTATC
BspLU11I 2101 ACATGT A^CATGT
3239 ACATGT
BsrDI 2644 CATTGC GCAATG(2/0)
5836 GCAATG
BstAPI 230 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2680 GCAAAGGCTGC
BstXI 397 CCAAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCAGGGCTCTGG
EcoNI 2349 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3064 CCTGTCTGAGG
EcoT22I 1740 ATGCAT ATGCA^T
5931 ATGCAT
HaeII 72 AGCGCC [GA]GCGC^[CT]
2811 AGCGCC
MamI 3544 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5168 GATGTTTATC
NsiI 1740 ATGCAT ATGCA^T
5931 ATGCAT
PpuMI 1671 AGGTCCT [GA]G^G[AT]CC[CT]
4041 GGGACCT
SacI 2441 GAGCTC GAGCT^C
3572 GAGCTC
XmnI 846 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2251 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AatII Acc65I AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII