################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AccII AclI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AvrII BbeI BbrPI BglI BlnI BsaAI BsiWI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI KasI KspI MfeI MluI MunI MvnI NarI NotI NruI PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 4686 TCCGGA T^CCGGA AflII 6242 CTTAAG C^TTAAG AgeI 1369 ACCGGT A^CCGGT Alw44I 1753 GTGCAC G^TGCAC ApaLI 1753 GTGCAC G^TGCAC AspI 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 238 TGCGCA TGC^GCA BamHI 1588 GGATCC G^GATCC BbvCI 1690 GCTGAGG CCTCAGC(-5/-2) BcgI 4755 GCAAAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 125 GTATCC GTATCC(6/5) BfrI 6242 CTTAAG C^TTAAG BlpI 2983 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2983 GCTGAGC GC^T[ACGT]AGC BseAI 4686 TCCGGA T^CCGGA BsiEI 47 CGGTCG CG[GA][CT]^CG BsmBI 5373 GAGACG CGTCTC(1/5) BspDI 3869 ATCGAT AT^CGAT BspEI 4686 TCCGGA T^CCGGA BspHI 2499 TCATGA T^CATGA BssSI 5867 CTCGTG CACGAG(-5/-1) BstBI 322 TTCGAA TT^CGAA CelII 2983 GCTGAGC GC^T[ACGT]AGC ClaI 3869 ATCGAT AT^CGAT DraIII 4019 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1272 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 238 TGCGCA TGC^GCA HgaI 55 GCGTC GACGC(5/10) HpaI 4027 GTTAAC GTT^AAC MroI 4686 TCCGGA T^CCGGA NaeI 3907 GCCGGC GCC^GGC NdeI 1081 CATATG CA^TATG NgoMIV 3907 GCCGGC G^CCGGC NheI 1211 GCTAGC G^CTAGC NspV 322 TTCGAA TT^CGAA PaeR7I 4653 CTCGAG C^TCGAG PflFI 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1369 ACCGGT A^CCGGT PshAI 3238 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2499 TCATGA T^CATGA SfuI 322 TTCGAA TT^CGAA SmaI 1647 CCCGGG CCC^GGG SphI 5818 GCATGC GCATG^C SspI 5286 AATATT AAT^ATT Tth111I 3044 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4653 CTCGAG C^TCGAG XmaCI 1647 CCCGGG C^CCGGG XmaI 1647 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 3413 GTAGAC GT^[AC][GT]AC 5497 GTAGAC AflIII 2101 ACATGT A^C[GA][CT]GT 3239 ACATGT Asp700I 846 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2251 GAAAGCTTTC BsaBI 3544 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5168 GATGTTTATC BspLU11I 2101 ACATGT A^CATGT 3239 ACATGT BsrDI 2644 CATTGC GCAATG(2/0) 5836 GCAATG BstAPI 230 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2680 GCAAAGGCTGC BstXI 397 CCAAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1911 CCAGGGCTCTGG EcoNI 2349 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3064 CCTGTCTGAGG EcoT22I 1740 ATGCAT ATGCA^T 5931 ATGCAT HaeII 72 AGCGCC [GA]GCGC^[CT] 2811 AGCGCC MamI 3544 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5168 GATGTTTATC NsiI 1740 ATGCAT ATGCA^T 5931 ATGCAT PpuMI 1671 AGGTCCT [GA]G^G[AT]CC[CT] 4041 GGGACCT SacI 2441 GAGCTC GAGCT^C 3572 GAGCTC XmnI 846 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2251 GAAAGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AatII Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII