################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII BamHI BbeI BbrPI BbvCI BciVI BfrI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I FseI FspI KasI MamI MroI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 3374 GACGCC G[GA]^CG[CT]C AocI 88 CCTGAGG CC^T[ACGT]AGG Aor51HI 2769 AGCGCT AGC^GCT AspI 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3170 CTCGGG C^[CT]CG[GA]G AvrII 906 CCTAGG C^CTAGG BbsI 2183 GTCTTC GAAGAC(2/6) BcgI 3499 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2652 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 906 CCTAGG C^CTAGG BlpI 2298 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2298 GCTAAGC GC^T[ACGT]AGC BpuAI 2183 GTCTTC GAAGAC(2/6) BsaHI 3374 GACGCC G[GA]^CG[CT]C BsaWI 3071 TCCGGT [AT]^CCGG[AT] BsiEI 2392 CGACCG CG[GA][CT]^CG BsoBI 3170 CTCGGG C^[CT]CG[GA]G BsrBI 2344 GAGCGG CCGCTC(-3/-3) BssSI 3058 CACGAG CACGAG(-5/-1) BstBI 4325 TTCGAA TT^CGAA Bsu36I 88 CCTGAGG CC^T[ACGT]AGG CelII 2298 GCTAAGC GC^T[ACGT]AGC DraIII 1855 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3785 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2769 AGCGCT AGC^GCT Eco81I 88 CCTGAGG CC^T[ACGT]AGG EcoRI 911 GAATTC G^AATTC EcoRV 2949 GATATC GAT^ATC EcoT22I 2674 ATGCAT ATGCA^T Hin1I 3374 GACGCC G[GA]^CG[CT]C KspI 2275 CCGCGG CCGC^GG MluI 4080 ACGCGT A^CGCGT NcoI 3433 CCATGG C^CATGG NdeI 4641 CATATG CA^TATG NheI 2793 GCTAGC G^CTAGC NsiI 2674 ATGCAT ATGCA^T NspV 4325 TTCGAA TT^CGAA PflFI 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3834 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 2549 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 4954 GAGCTC GAGCT^C SacII 2275 CCGCGG CCGC^GG SexAI 1397 ACCAGGT A^CC[AT]GGT SfuI 4325 TTCGAA TT^CGAA Tth111I 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3834 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3564 GGTACC G^GTACC 5213 GGTACC AflIII 1975 ACACGT A^C[GA][CT]GT 4080 ACGCGT Alw44I 737 GTGCAC G^TGCAC 4535 GTGCAC ApaI 1463 GGGCCC GGGCC^C 3724 GGGCCC ApaLI 737 GTGCAC G^TGCAC 4535 GTGCAC Asp700I 644 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4328 GAAAGGTTTC Asp718I 3564 GGTACC G^GTACC 5213 GGTACC BalI 413 TGGCCA TGG^CCA 3532 TGGCCA BclI 4737 TGATCA T^GATCA 5118 TGATCA BsaAI 196 TACGTA [CT]AC^GT[GA] 4886 TACGTA BsmBI 3130 CGTCTC CGTCTC(1/5) 3526 CGTCTC BsmI 3596 GAATGC GAATGC(1/-1) 3760 GAATGC Bsp1407I 1911 TGTACA T^GTACA 5024 TGTACA BspHI 397 TCATGA T^CATGA 2049 TCATGA BspMI 3337 ACCTGC ACCTGC(4/8) 3887 ACCTGC BsrDI 2748 GCAATG GCAATG(2/0) 3486 GCAATG BsrGI 1911 TGTACA T^GTACA 5024 TGTACA BstXI 2839 CCACGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3651 CCAGCCACATGG EaeI 413 TGGCCA [CT]^GGCC[GA] 3532 TGGCCA EcoNI 3744 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3765 CCTCCTTCAGG FbaI 4737 TGATCA T^GATCA 5118 TGATCA HaeII 2769 AGCGCT [GA]GCGC^[CT] 3125 AGCGCC HindIII 1032 AAGCTT A^AGCTT 1996 AAGCTT HpaI 545 GTTAAC GTT^AAC 1135 GTTAAC KpnI 3564 GGTACC GGTAC^C 5213 GGTACC MfeI 1588 CAATTG C^AATTG 4949 CAATTG MluNI 413 TGGCCA TGG^CCA 3532 TGGCCA MscI 413 TGGCCA TGG^CCA 3532 TGGCCA MunI 1588 CAATTG C^AATTG 4949 CAATTG PpuMI 1556 GGGTCCC [GA]G^G[AT]CC[CT] 3922 AGGACCC RcaI 397 TCATGA T^CATGA 2049 TCATGA SapI 173 GCTCTTC GCTCTTC(1/4) 4160 GCTCTTC SmlI 1999 CTTGAG C^T[CT][GA]AG 4363 CTCAAG SnaBI 196 TACGTA TAC^GTA 4886 TACGTA SphI 7 GCATGC GCATG^C 2676 GCATGC SspBI 1911 TGTACA T^GTACA 5024 TGTACA SspI 1695 AATATT AAT^ATT 4861 AATATT StuI 519 AGGCCT AGG^CCT 3503 AGGCCT XbaI 2138 TCTAGA T^CTAGA 2945 TCTAGA XmnI 644 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4328 GAAAGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII