##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
AspEI AviII BamHI BbeI BbrPI
BbvCI BciVI BfrI BsaBI BseAI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstEII BstPI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I FseI FspI
KasI MamI MroI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 3374 GACGCC G[GA]^CG[CT]C
AocI 88 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2769 AGCGCT AGC^GCT
AspI 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3170 CTCGGG C^[CT]CG[GA]G
AvrII 906 CCTAGG C^CTAGG
BbsI 2183 GTCTTC GAAGAC(2/6)
BcgI 3499 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2652 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 906 CCTAGG C^CTAGG
BlpI 2298 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2298 GCTAAGC GC^T[ACGT]AGC
BpuAI 2183 GTCTTC GAAGAC(2/6)
BsaHI 3374 GACGCC G[GA]^CG[CT]C
BsaWI 3071 TCCGGT [AT]^CCGG[AT]
BsiEI 2392 CGACCG CG[GA][CT]^CG
BsoBI 3170 CTCGGG C^[CT]CG[GA]G
BsrBI 2344 GAGCGG CCGCTC(-3/-3)
BssSI 3058 CACGAG CACGAG(-5/-1)
BstBI 4325 TTCGAA TT^CGAA
Bsu36I 88 CCTGAGG CC^T[ACGT]AGG
CelII 2298 GCTAAGC GC^T[ACGT]AGC
DraIII 1855 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3785 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2769 AGCGCT AGC^GCT
Eco81I 88 CCTGAGG CC^T[ACGT]AGG
EcoRI 911 GAATTC G^AATTC
EcoRV 2949 GATATC GAT^ATC
EcoT22I 2674 ATGCAT ATGCA^T
Hin1I 3374 GACGCC G[GA]^CG[CT]C
KspI 2275 CCGCGG CCGC^GG
MluI 4080 ACGCGT A^CGCGT
NcoI 3433 CCATGG C^CATGG
NdeI 4641 CATATG CA^TATG
NheI 2793 GCTAGC G^CTAGC
NsiI 2674 ATGCAT ATGCA^T
NspV 4325 TTCGAA TT^CGAA
PflFI 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3834 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2549 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4954 GAGCTC GAGCT^C
SacII 2275 CCGCGG CCGC^GG
SexAI 1397 ACCAGGT A^CC[AT]GGT
SfuI 4325 TTCGAA TT^CGAA
Tth111I 1439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3834 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3564 GGTACC G^GTACC
5213 GGTACC
AflIII 1975 ACACGT A^C[GA][CT]GT
4080 ACGCGT
Alw44I 737 GTGCAC G^TGCAC
4535 GTGCAC
ApaI 1463 GGGCCC GGGCC^C
3724 GGGCCC
ApaLI 737 GTGCAC G^TGCAC
4535 GTGCAC
Asp700I 644 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4328 GAAAGGTTTC
Asp718I 3564 GGTACC G^GTACC
5213 GGTACC
BalI 413 TGGCCA TGG^CCA
3532 TGGCCA
BclI 4737 TGATCA T^GATCA
5118 TGATCA
BsaAI 196 TACGTA [CT]AC^GT[GA]
4886 TACGTA
BsmBI 3130 CGTCTC CGTCTC(1/5)
3526 CGTCTC
BsmI 3596 GAATGC GAATGC(1/-1)
3760 GAATGC
Bsp1407I 1911 TGTACA T^GTACA
5024 TGTACA
BspHI 397 TCATGA T^CATGA
2049 TCATGA
BspMI 3337 ACCTGC ACCTGC(4/8)
3887 ACCTGC
BsrDI 2748 GCAATG GCAATG(2/0)
3486 GCAATG
BsrGI 1911 TGTACA T^GTACA
5024 TGTACA
BstXI 2839 CCACGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3651 CCAGCCACATGG
EaeI 413 TGGCCA [CT]^GGCC[GA]
3532 TGGCCA
EcoNI 3744 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3765 CCTCCTTCAGG
FbaI 4737 TGATCA T^GATCA
5118 TGATCA
HaeII 2769 AGCGCT [GA]GCGC^[CT]
3125 AGCGCC
HindIII 1032 AAGCTT A^AGCTT
1996 AAGCTT
HpaI 545 GTTAAC GTT^AAC
1135 GTTAAC
KpnI 3564 GGTACC GGTAC^C
5213 GGTACC
MfeI 1588 CAATTG C^AATTG
4949 CAATTG
MluNI 413 TGGCCA TGG^CCA
3532 TGGCCA
MscI 413 TGGCCA TGG^CCA
3532 TGGCCA
MunI 1588 CAATTG C^AATTG
4949 CAATTG
PpuMI 1556 GGGTCCC [GA]G^G[AT]CC[CT]
3922 AGGACCC
RcaI 397 TCATGA T^CATGA
2049 TCATGA
SapI 173 GCTCTTC GCTCTTC(1/4)
4160 GCTCTTC
SmlI 1999 CTTGAG C^T[CT][GA]AG
4363 CTCAAG
SnaBI 196 TACGTA TAC^GTA
4886 TACGTA
SphI 7 GCATGC GCATG^C
2676 GCATGC
SspBI 1911 TGTACA T^GTACA
5024 TGTACA
SspI 1695 AATATT AAT^ATT
4861 AATATT
StuI 519 AGGCCT AGG^CCT
3503 AGGCCT
XbaI 2138 TCTAGA T^CTAGA
2945 TCTAGA
XmnI 644 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4328 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII