##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AseI AsnI AviII AvrII BalI
BciVI BclI BlnI BsaWI BseAI
BsiWI BspDI BspEI BsrFI BssSI
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI Eco47III EcoRV FbaI
FseI FspI HpaI MluI MluNI
MroI MscI NaeI NcoI NgoMIV
NheI NotI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SbfI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1211 GACGTC GACGT^C
AflII 971 CTTAAG C^TTAAG
AflIII 3741 ACATGT A^C[GA][CT]GT
Alw44I 1862 GTGCAC G^TGCAC
ApaLI 1862 GTGCAC G^TGCAC
Asp700I 1937 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4434 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3535 GGATCC G^GATCC
BbeI 1954 GGCGCC GGCGC^C
BbrPI 4025 CACGTG CAC^GTG
BcgI 292 GCAGAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 971 CTTAAG C^TTAAG
BglI 3027 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4025 CACGTG [CT]AC^GT[GA]
BsaBI 1346 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 3741 ACATGT A^CATGT
BsrDI 4396 CATTGC GCAATG(2/0)
BssHII 4036 GCGCGC G^CGCGC
BstEII 4371 GGTCACC G^GT[ACGT]ACC
BstPI 4371 GGTCACC G^GT[ACGT]ACC
DrdI 103 GACCACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 557 CCGCGG C^C[GA][CT]GG
EagI 2984 CGGCCG C^GGCCG
EclXI 2984 CGGCCG C^GGCCG
Eco52I 2984 CGGCCG C^GGCCG
EcoNI 502 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4371 GGTCACC G^GT[ACGT]ACC
EcoRI 2202 GAATTC G^AATTC
EcoT22I 1453 ATGCAT ATGCA^T
HindIII 4518 AAGCTT A^AGCTT
KasI 1954 GGCGCC G^GCGCC
KspI 557 CCGCGG CCGC^GG
MamI 1346 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1178 CAATTG C^AATTG
MunI 1178 CAATTG C^AATTG
NarI 1954 GGCGCC GG^CGCC
NdeI 3937 CATATG CA^TATG
NruI 541 TCGCGA TCG^CGA
NsiI 1453 ATGCAT ATGCA^T
PflFI 4434 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4362 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4025 CACGTG CAC^GTG
PmlI 4025 CACGTG CAC^GTG
PshAI 1443 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 557 CCGCGG CCGC^GG
ScaI 4444 AGTACT AGT^ACT
SfoI 1954 GGCGCC GGC^GCC
SmaI 475 CCCGGG CCC^GGG
SpeI 4457 ACTAGT A^CTAGT
SspI 4505 AATATT AAT^ATT
Tth111I 4434 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4362 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 475 CCCGGG C^CCGGG
XmaI 475 CCCGGG C^CCGGG
XmnI 1937 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 3305 GGTACC G^GTACC
3314 GGTACC
AccI 300 GTCGAC GT^[AC][GT]AC
2558 GTCGAC
AlwNI 305 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2893 CAGGCCCTG
AocI 2890 CCTCAGG CC^T[ACGT]AGG
3132 CCTGAGG
Asp718I 3305 GGTACC G^GTACC
3314 GGTACC
BbsI 3763 GAAGAC GAAGAC(2/6)
1214 GTCTTC
BglII 2608 AGATCT A^GATCT
3663 AGATCT
BlpI 2180 GCTCAGC GC^T[ACGT]AGC
2315 GCTAAGC
Bpu1102I 2180 GCTCAGC GC^T[ACGT]AGC
2315 GCTAAGC
BpuAI 3763 GAAGAC GAAGAC(2/6)
1214 GTCTTC
BsgI 661 GTGCAG GTGCAG(16/14)
4278 GTGCAG
BsiEI 544 CGACCG CG[GA][CT]^CG
2984 CGGCCG
Bsp1407I 2349 TGTACA T^GTACA
3734 TGTACA
BsrBI 255 GAGCGG CCGCTC(-3/-3)
284 GAGCGG
BsrGI 2349 TGTACA T^GTACA
3734 TGTACA
BstXI 3156 CCAGTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3581 CCAGAGAAATGG
Bsu36I 2890 CCTCAGG CC^T[ACGT]AGG
3132 CCTGAGG
CelII 2180 GCTCAGC GC^T[ACGT]AGC
2315 GCTAAGC
DraIII 3011 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4363 CACTGGGTG
EaeI 2011 CGGCCA [CT]^GGCC[GA]
2984 CGGCCG
Eco81I 2890 CCTCAGG CC^T[ACGT]AGG
3132 CCTGAGG
HgaI 612 GACGC GACGC(5/10)
3806 GCGTC
HincII 300 GTCGAC GT[CT]^[GA]AC
2558 GTCGAC
HindII 300 GTCGAC GT[CT]^[GA]AC
2558 GTCGAC
KpnI 3305 GGTACC GGTAC^C
3314 GGTACC
SalI 300 GTCGAC G^TCGAC
2558 GTCGAC
SapI 1675 GAAGAGC GCTCTTC(1/4)
1922 GCTCTTC
SphI 3950 GCATGC GCATG^C
3958 GCATGC
SspBI 2349 TGTACA T^GTACA
3734 TGTACA
XcmI 60 CCAGGACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2606 CCAGATCTGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI ApaI ApoI
AspEI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII