##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AspI BcgI
BciVI BclI BfrI BglII BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HpaI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2759 GGTACC G^GTACC
AcyI 2615 GGCGCC G[GA]^CG[CT]C
AflIII 1220 ACGTGT A^C[GA][CT]GT
Alw44I 1097 GTGCAC G^TGCAC
ApaLI 1097 GTGCAC G^TGCAC
Asp718I 2759 GGTACC G^GTACC
AviII 1079 TGCGCA TGC^GCA
AvrII 586 CCTAGG C^CTAGG
BalI 50 TGGCCA TGG^CCA
BbeI 2615 GGCGCC GGCGC^C
BbrPI 328 CACGTG CAC^GTG
BbvCI 1852 GCTGAGG CCTCAGC(-5/-2)
BlnI 586 CCTAGG C^CTAGG
BsaAI 328 CACGTG [CT]AC^GT[GA]
BsaHI 2615 GGCGCC G[GA]^CG[CT]C
BsaWI 2201 ACCGGA [AT]^CCGG[AT]
BsmBI 2551 GAGACG CGTCTC(1/5)
BsmI 261 GAATGC GAATGC(1/-1)
BstAPI 2517 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 3415 TTTAAA TTT^AAA
DsaI 1277 CCACGG C^C[GA][CT]GG
EcoNI 2284 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 398 GAATTC G^AATTC
FspI 1079 TGCGCA TGC^GCA
Hin1I 2615 GGCGCC G[GA]^CG[CT]C
HindIII 2449 AAGCTT A^AGCTT
KasI 2615 GGCGCC G^GCGCC
KpnI 2759 GGTACC GGTAC^C
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MseI 3416 TTAA T^TAA
NarI 2615 GGCGCC GG^CGCC
PmaCI 328 CACGTG CAC^GTG
PmlI 328 CACGTG CAC^GTG
SacI 365 GAGCTC GAGCT^C
SalI 191 GTCGAC G^TCGAC
ScaI 1163 AGTACT AGT^ACT
SfiI 2529 GGCCCGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2615 GGCGCC GGC^GCC
SmaI 1838 CCCGGG CCC^GGG
SphI 911 GCATGC GCATG^C
SspI 1985 AATATT AAT^ATT
TfiI 2430 GATTC G^A[AT]TC
Tru9I 3416 TTAA T^TAA
XbaI 1446 TCTAGA T^CTAGA
XcmI 2763 CCAGAAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1838 CCCGGG C^CCGGG
XmaI 1838 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 32 GTCTAC GT^[AC][GT]AC
191 GTCGAC
AocI 832 CCTGAGG CC^T[ACGT]AGG
2008 CCTAAGG
AvaI 485 CTCGGG C^[CT]CG[GA]G
1838 CCCGGG
BbsI 2251 GAAGAC GAAGAC(2/6)
106 GTCTTC
BpuAI 2251 GAAGAC GAAGAC(2/6)
106 GTCTTC
BsaI 783 GGTCTC GGTCTC(1/5)
1028 GGTCTC
BsoBI 485 CTCGGG C^[CT]CG[GA]G
1838 CCCGGG
BstXI 1216 CCAGACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2156 CCAAACAGTTGG
Bsu36I 832 CCTGAGG CC^T[ACGT]AGG
2008 CCTAAGG
Eco81I 832 CCTGAGG CC^T[ACGT]AGG
2008 CCTAAGG
HgaI 279 GACGC GACGC(5/10)
1608 GACGC
HincII 191 GTCGAC GT[CT]^[GA]AC
1480 GTCAAC
HindII 191 GTCGAC GT[CT]^[GA]AC
1480 GTCAAC
NspI 911 GCATGC [GA]CATG^[CT]
2003 ACATGC
StuI 885 AGGCCT AGG^CCT
2334 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII