##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I BbeI BbrPI BfrI BsaBI
BsiEI BsiWI BspDI BssHII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoNI FseI HaeII HpaI KasI
KpnI KspI MamI MfeI MunI
NaeI NarI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3834 GACGTC GACGT^C
AcyI 3834 GACGTC G[GA]^CG[CT]C
ApaI 3378 GGGCCC GGGCC^C
AspI 1528 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2240 TGCGCA TGC^GCA
BbvCI 2124 GCTGAGG CCTCAGC(-5/-2)
BcgI 3650 CGATCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1846 CACGTA [CT]AC^GT[GA]
BsaHI 3834 GACGTC G[GA]^CG[CT]C
Bsp1407I 2726 TGTACA T^GTACA
BspHI 1317 TCATGA T^CATGA
BsrFI 3577 ACCGGC [GA]^CCGG[CT]
BsrGI 2726 TGTACA T^GTACA
BstBI 2593 TTCGAA TT^CGAA
Cfr10I 3577 ACCGGC [GA]^CCGG[CT]
DraI 3173 TTTAAA TTT^AAA
DrdI 6089 GACTGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 422 GATATC GAT^ATC
FspI 2240 TGCGCA TGC^GCA
Hin1I 3834 GACGTC G[GA]^CG[CT]C
HincII 6772 GTCAAC GT[CT]^[GA]AC
HindII 6772 GTCAAC GT[CT]^[GA]AC
HindIII 574 AAGCTT A^AGCTT
MluI 2498 ACGCGT A^CGCGT
NspV 2593 TTCGAA TT^CGAA
PaeR7I 208 CTCGAG C^TCGAG
PflFI 1528 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6554 CCACGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5896 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1317 TCATGA T^CATGA
SfuI 2593 TTCGAA TT^CGAA
SmaI 5378 CCCGGG CCC^GGG
SpeI 361 ACTAGT A^CTAGT
SspBI 2726 TGTACA T^GTACA
Tth111I 1528 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6554 CCACGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 208 CTCGAG C^TCGAG
XmaCI 5378 CCCGGG C^CCGGG
XmaI 5378 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 2499 CGCG CG^CG
3648 CGCG
AccIII 3871 TCCGGA T^CCGGA
5504 TCCGGA
AhdI 2520 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4909 GACCTGCAGTC
AspEI 2520 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4909 GACCTGCAGTC
AvrII 735 CCTAGG C^CTAGG
1536 CCTAGG
BciVI 1209 GGATAC GTATCC(6/5)
4015 GTATCC
BclI 861 TGATCA T^GATCA
6537 TGATCA
BlnI 735 CCTAGG C^CTAGG
1536 CCTAGG
BsaI 809 GAGACC GGTCTC(1/5)
1564 GAGACC
BseAI 3871 TCCGGA T^CCGGA
5504 TCCGGA
BsmBI 3026 CGTCTC CGTCTC(1/5)
5296 GAGACG
BspEI 3871 TCCGGA T^CCGGA
5504 TCCGGA
BsrDI 4871 GCAATG GCAATG(2/0)
5525 GCAATG
BssSI 3567 CACGAG CACGAG(-5/-1)
1922 CTCGTG
Bst1107I 479 GTATAC GTA^TAC
4941 GTATAC
BstEII 1614 GGTTACC G^GT[ACGT]ACC
1680 GGTGACC
BstPI 1614 GGTTACC G^GT[ACGT]ACC
1680 GGTGACC
BstUI 2499 CGCG CG^CG
3648 CGCG
BstZ17I 479 GTATAC GTA^TAC
4941 GTATAC
Eam1105I 2520 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4909 GACCTGCAGTC
EcoO65I 1614 GGTTACC G^GT[ACGT]ACC
1680 GGTGACC
FbaI 861 TGATCA T^GATCA
6537 TGATCA
HgaI 2912 GACGC GACGC(5/10)
5820 GACGC
MroI 3871 TCCGGA T^CCGGA
5504 TCCGGA
MvnI 2499 CGCG CG^CG
3648 CGCG
NdeI 3298 CATATG CA^TATG
5476 CATATG
NheI 1162 GCTAGC G^CTAGC
5675 GCTAGC
SacI 2108 GAGCTC GAGCT^C
4379 GAGCTC
SapI 172 GAAGAGC GCTCTTC(1/4)
5883 GCTCTTC
ScaI 329 AGTACT AGT^ACT
6276 AGTACT
SspI 48 AATATT AAT^ATT
6232 AATATT
XbaI 3365 TCTAGA T^CTAGA
6446 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI