##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI BbeI
BbrPI BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoT22I HpaI
KasI KspI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 5942 GACGTC GACGT^C
Acc65I 4477 GGTACC G^GTACC
AcyI 5942 GACGTC G[GA]^CG[CT]C
AflII 4210 CTTAAG C^TTAAG
AflIII 3805 ACATGT A^C[GA][CT]GT
AhdI 5129 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2863 AGCGCT AGC^GCT
ApaI 6330 GGGCCC GGGCC^C
Asp718I 4477 GGTACC G^GTACC
AspEI 5129 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4913 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1643 TGCGCA TGC^GCA
AvrII 4826 CCTAGG C^CTAGG
BalI 796 TGGCCA TGG^CCA
BanI 4477 GGTACC G^G[CT][GA]CC
BcgI 5953 GCACAGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4210 CTTAAG C^TTAAG
BlnI 4826 CCTAGG C^CTAGG
BsaHI 5942 GACGTC G[GA]^CG[CT]C
BsaWI 5482 TCCGGT [AT]^CCGG[AT]
BsmBI 3908 GAGACG CGTCTC(1/5)
BspLU11I 3805 ACATGT A^CATGT
BssHII 116 GCGCGC G^CGCGC
BssSI 3762 CTCGTG CACGAG(-5/-1)
BstXI 2049 CCACACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4031 TTTAAA TTT^AAA
DraIII 3849 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 5999 CCATGG C^C[GA][CT]GG
Eam1105I 5129 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2863 AGCGCT AGC^GCT
EcoNI 6321 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2371 GATATC GAT^ATC
FseI 4187 GGCCGGCC GGCCGG^CC
FspI 1643 TGCGCA TGC^GCA
HgaI 2146 GCGTC GACGC(5/10)
Hin1I 5942 GACGTC G[GA]^CG[CT]C
KpnI 4477 GGTACC GGTAC^C
MluNI 796 TGGCCA TGG^CCA
MscI 796 TGGCCA TGG^CCA
NaeI 4188 GCCGGC GCC^GGC
NcoI 5999 CCATGG C^CATGG
NdeI 2648 CATATG CA^TATG
NgoMIV 4188 GCCGGC G^CCGGC
NspI 3805 ACATGT [GA]CATG^[CT]
PaeR7I 60 CTCGAG C^TCGAG
PflFI 4913 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 558 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1112 GAGCTC GAGCT^C
ScaI 1226 AGTACT AGT^ACT
SmaI 5824 CCCGGG CCC^GGG
SpeI 1229 ACTAGT A^CTAGT
SspI 428 AATATT AAT^ATT
Tth111I 4913 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 558 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3581 TCTAGA T^CTAGA
XcmI 4771 CCAGAATCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 60 CTCGAG C^TCGAG
XmaCI 5824 CCCGGG C^CCGGG
XmaI 5824 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 117 CGCG CG^CG
5353 CGCG
AocI 2963 CCTAAGG CC^T[ACGT]AGG
4893 CCTCAGG
Asp700I 2892 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4737 GAAGTTTTTC
BamHI 2359 GGATCC G^GATCC
4177 GGATCC
BciVI 3668 GGATAC GTATCC(6/5)
3769 GTATCC
BglI 4132 GCCTACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4375 GCCCCGAAGGC
BlpI 1343 GCTCAGC GC^T[ACGT]AGC
2567 GCTGAGC
Bpu1102I 1343 GCTCAGC GC^T[ACGT]AGC
2567 GCTGAGC
BsmI 363 GAATGC GAATGC(1/-1)
2923 GAATGC
BspHI 2340 TCATGA T^CATGA
3931 TCATGA
BsrFI 4188 GCCGGC [GA]^CCGG[CT]
4318 ACCGGC
BstEII 762 GGTCACC G^GT[ACGT]ACC
2460 GGTCACC
BstPI 762 GGTCACC G^GT[ACGT]ACC
2460 GGTCACC
BstUI 117 CGCG CG^CG
5353 CGCG
Bsu36I 2963 CCTAAGG CC^T[ACGT]AGG
4893 CCTCAGG
CelII 1343 GCTCAGC GC^T[ACGT]AGC
2567 GCTGAGC
Cfr10I 4188 GCCGGC [GA]^CCGG[CT]
4318 ACCGGC
EaeI 796 TGGCCA [CT]^GGCC[GA]
4190 CGGCCA
Eco81I 2963 CCTAAGG CC^T[ACGT]AGG
4893 CCTCAGG
EcoO65I 762 GGTCACC G^GT[ACGT]ACC
2460 GGTCACC
EcoRI 4573 GAATTC G^AATTC
4975 GAATTC
FauI 1738 GCGGG CCCGC(4/6)
1768 GCGGG
HaeII 2863 AGCGCT [GA]GCGC^[CT]
5891 AGCGCC
HincII 2874 GTCAAC GT[CT]^[GA]AC
5470 GTCAAC
HindII 2874 GTCAAC GT[CT]^[GA]AC
5470 GTCAAC
MvnI 117 CGCG CG^CG
5353 CGCG
PshAI 5599 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6357 GACTTGAGTC
RcaI 2340 TCATGA T^CATGA
3931 TCATGA
SexAI 4108 ACCTGGT A^CC[AT]GGT
6389 ACCTGGT
XmnI 2892 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4737 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII