##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AccII       AccIII      AclI        AgeI        Aor51HI     
AscI        BcgI        BseAI       BspDI       BspEI       
BssHII      BstBI       BstUI       ClaI        CpoI        
Eco47III    EcoRV       FseI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SbfI        SexAI       SfiI        SfuI        SgrAI       
SpeI        Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       2770  GGTACC               G^GTACC
AflIII        882  ACATGT               A^C[GA][CT]GT
Alw44I       1728  GTGCAC               G^TGCAC
ApaI           73  GGGCCC               GGGCC^C
ApaLI        1728  GTGCAC               G^TGCAC
AseI         5373  ATTAAT               AT^TAAT
AsnI         5373  ATTAAT               AT^TAAT
Asp718I      2770  GGTACC               G^GTACC
AviII        5425  TGCGCA               TGC^GCA
AvrII        2898  CCTAGG               C^CTAGG
BbeI         1497  GGCGCC               GGCGC^C
BbrPI         283  CACGTG               CAC^GTG
BglI          694  GCCTACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2898  CCTAGG               C^CTAGG
BlpI          807  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      807  GCTCAGC              GC^T[ACGT]AGC
BsaI         4184  GGTCTC               GGTCTC(1/5)
BsiEI        2006  CGGCCG               CG[GA][CT]^CG
BsiWI        1073  CGTACG               C^GTACG
BsmI         5163  GCATTC               GAATGC(1/-1)
BspLU11I      882  ACATGT               A^CATGT
BstAPI       1512  GCAGGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         807  GCTCAGC              GC^T[ACGT]AGC
EagI         2006  CGGCCG               C^GGCCG
EclXI        2006  CGGCCG               C^GGCCG
Eco52I       2006  CGGCCG               C^GGCCG
FspI         5425  TGCGCA               TGC^GCA
HindIII       781  AAGCTT               A^AGCTT
HpaI         3250  GTTAAC               GTT^AAC
KasI         1497  GGCGCC               G^GCGCC
KpnI         2770  GGTACC               GGTAC^C
NarI         1497  GGCGCC               GG^CGCC
PmaCI         283  CACGTG               CAC^GTG
PmlI          283  CACGTG               CAC^GTG
PshBI        5373  ATTAAT               AT^TAAT
PvuII        1822  CAGCTG               CAG^CTG
SacI          805  GAGCTC               GAGCT^C
SalI         2011  GTCGAC               G^TCGAC
SapI         1317  GAAGAGC              GCTCTTC(1/4)
SfoI         1497  GGCGCC               GGC^GCC
SmaI           18  CCCGGG               CCC^GGG
XbaI         4644  TCTAGA               T^CTAGA
XmaCI          18  CCCGGG               C^CCGGG
XmaI           18  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII         625  GACGTC               GACGT^C
             1735  GACGTC              
AflII        4868  CTTAAG               C^TTAAG
             5451  CTTAAG              
AocI         3565  CCTCAGG              CC^T[ACGT]AGG
             4199  CCTCAGG             
AspI          178  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              231  GACCACGTC           
BamHI        1078  GGATCC               G^GATCC
             2287  GGATCC              
BciVI         278  GTATCC               GTATCC(6/5)
             1453  GTATCC              
BclI         4535  TGATCA               T^GATCA
             4727  TGATCA              
BfrI         4868  CTTAAG               C^TTAAG
             5451  CTTAAG              
BsaBI        1548  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4880  GATAGAAATC          
BsaWI           3  ACCGGA               [AT]^CCGG[AT]
             2230  ACCGGA              
BseRI        3740  CTCCTC               GAGGAG(10/8)
             5198  GAGGAG              
BsmBI         183  CGTCTC               CGTCTC(1/5)
               65  GAGACG              
BspHI         680  TCATGA               T^CATGA
             4724  TCATGA              
BsrBI         857  GAGCGG               CCGCTC(-3/-3)
             1929  GAGCGG              
BsrFI         896  ACCGGC               [GA]^CCGG[CT]
             1631  GCCGGT              
BssSI        4325  CACGAG               CACGAG(-5/-1)
              205  CTCGTG              
Bst1107I     2808  GTATAC               GTA^TAC
             2991  GTATAC              
BstXI         372  CCACAGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2089  CCAGCCCCCTGG        
BstZ17I      2808  GTATAC               GTA^TAC
             2991  GTATAC              
Bsu36I       3565  CCTCAGG              CC^T[ACGT]AGG
             4199  CCTCAGG             
Cfr10I        896  ACCGGC               [GA]^CCGG[CT]
             1631  GCCGGT              
Eco81I       3565  CCTCAGG              CC^T[ACGT]AGG
             4199  CCTCAGG             
FbaI         4535  TGATCA               T^GATCA
             4727  TGATCA              
HaeII        1497  GGCGCC               [GA]GCGC^[CT]
             1588  GGCGCT              
HgaI           67  GACGC                GACGC(5/10)
             2394  GACGC               
MamI         1548  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4880  GATAGAAATC          
PflFI         178  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              231  GACCACGTC           
RcaI          680  TCATGA               T^CATGA
             4724  TCATGA              
ScaI          560  AGTACT               AGT^ACT
             4742  AGTACT              
SnaBI        3366  TACGTA               TAC^GTA
             5432  TACGTA              
SspI         1122  AATATT               AAT^ATT
             3293  AATATT              
StuI          705  AGGCCT               AGG^CCT
             3391  AGGCCT              
Tth111I       178  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              231  GACCACGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApoI        
Asp700I     AspEI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrDI       BsrGI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
Eam1105I    EarI        EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmnI