##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccII AccIII AclI AgeI Aor51HI
AscI BcgI BseAI BspDI BspEI
BssHII BstBI BstUI ClaI CpoI
Eco47III EcoRV FseI KspI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2770 GGTACC G^GTACC
AflIII 882 ACATGT A^C[GA][CT]GT
Alw44I 1728 GTGCAC G^TGCAC
ApaI 73 GGGCCC GGGCC^C
ApaLI 1728 GTGCAC G^TGCAC
AseI 5373 ATTAAT AT^TAAT
AsnI 5373 ATTAAT AT^TAAT
Asp718I 2770 GGTACC G^GTACC
AviII 5425 TGCGCA TGC^GCA
AvrII 2898 CCTAGG C^CTAGG
BbeI 1497 GGCGCC GGCGC^C
BbrPI 283 CACGTG CAC^GTG
BglI 694 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2898 CCTAGG C^CTAGG
BlpI 807 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 807 GCTCAGC GC^T[ACGT]AGC
BsaI 4184 GGTCTC GGTCTC(1/5)
BsiEI 2006 CGGCCG CG[GA][CT]^CG
BsiWI 1073 CGTACG C^GTACG
BsmI 5163 GCATTC GAATGC(1/-1)
BspLU11I 882 ACATGT A^CATGT
BstAPI 1512 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 807 GCTCAGC GC^T[ACGT]AGC
EagI 2006 CGGCCG C^GGCCG
EclXI 2006 CGGCCG C^GGCCG
Eco52I 2006 CGGCCG C^GGCCG
FspI 5425 TGCGCA TGC^GCA
HindIII 781 AAGCTT A^AGCTT
HpaI 3250 GTTAAC GTT^AAC
KasI 1497 GGCGCC G^GCGCC
KpnI 2770 GGTACC GGTAC^C
NarI 1497 GGCGCC GG^CGCC
PmaCI 283 CACGTG CAC^GTG
PmlI 283 CACGTG CAC^GTG
PshBI 5373 ATTAAT AT^TAAT
PvuII 1822 CAGCTG CAG^CTG
SacI 805 GAGCTC GAGCT^C
SalI 2011 GTCGAC G^TCGAC
SapI 1317 GAAGAGC GCTCTTC(1/4)
SfoI 1497 GGCGCC GGC^GCC
SmaI 18 CCCGGG CCC^GGG
XbaI 4644 TCTAGA T^CTAGA
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 625 GACGTC GACGT^C
1735 GACGTC
AflII 4868 CTTAAG C^TTAAG
5451 CTTAAG
AocI 3565 CCTCAGG CC^T[ACGT]AGG
4199 CCTCAGG
AspI 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
231 GACCACGTC
BamHI 1078 GGATCC G^GATCC
2287 GGATCC
BciVI 278 GTATCC GTATCC(6/5)
1453 GTATCC
BclI 4535 TGATCA T^GATCA
4727 TGATCA
BfrI 4868 CTTAAG C^TTAAG
5451 CTTAAG
BsaBI 1548 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4880 GATAGAAATC
BsaWI 3 ACCGGA [AT]^CCGG[AT]
2230 ACCGGA
BseRI 3740 CTCCTC GAGGAG(10/8)
5198 GAGGAG
BsmBI 183 CGTCTC CGTCTC(1/5)
65 GAGACG
BspHI 680 TCATGA T^CATGA
4724 TCATGA
BsrBI 857 GAGCGG CCGCTC(-3/-3)
1929 GAGCGG
BsrFI 896 ACCGGC [GA]^CCGG[CT]
1631 GCCGGT
BssSI 4325 CACGAG CACGAG(-5/-1)
205 CTCGTG
Bst1107I 2808 GTATAC GTA^TAC
2991 GTATAC
BstXI 372 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2089 CCAGCCCCCTGG
BstZ17I 2808 GTATAC GTA^TAC
2991 GTATAC
Bsu36I 3565 CCTCAGG CC^T[ACGT]AGG
4199 CCTCAGG
Cfr10I 896 ACCGGC [GA]^CCGG[CT]
1631 GCCGGT
Eco81I 3565 CCTCAGG CC^T[ACGT]AGG
4199 CCTCAGG
FbaI 4535 TGATCA T^GATCA
4727 TGATCA
HaeII 1497 GGCGCC [GA]GCGC^[CT]
1588 GGCGCT
HgaI 67 GACGC GACGC(5/10)
2394 GACGC
MamI 1548 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4880 GATAGAAATC
PflFI 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
231 GACCACGTC
RcaI 680 TCATGA T^CATGA
4724 TCATGA
ScaI 560 AGTACT AGT^ACT
4742 AGTACT
SnaBI 3366 TACGTA TAC^GTA
5432 TACGTA
SspI 1122 AATATT AAT^ATT
3293 AATATT
StuI 705 AGGCCT AGG^CCT
3391 AGGCCT
Tth111I 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
231 GACCACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI