################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AccII AccIII AclI AgeI Aor51HI AscI BcgI BseAI BspDI BspEI BssHII BstBI BstUI ClaI CpoI Eco47III EcoRV FseI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfuI SgrAI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 2770 GGTACC G^GTACC AflIII 882 ACATGT A^C[GA][CT]GT Alw44I 1728 GTGCAC G^TGCAC ApaI 73 GGGCCC GGGCC^C ApaLI 1728 GTGCAC G^TGCAC AseI 5373 ATTAAT AT^TAAT AsnI 5373 ATTAAT AT^TAAT Asp718I 2770 GGTACC G^GTACC AviII 5425 TGCGCA TGC^GCA AvrII 2898 CCTAGG C^CTAGG BbeI 1497 GGCGCC GGCGC^C BbrPI 283 CACGTG CAC^GTG BglI 694 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2898 CCTAGG C^CTAGG BlpI 807 GCTCAGC GC^T[ACGT]AGC Bpu1102I 807 GCTCAGC GC^T[ACGT]AGC BsaI 4184 GGTCTC GGTCTC(1/5) BsiEI 2006 CGGCCG CG[GA][CT]^CG BsiWI 1073 CGTACG C^GTACG BsmI 5163 GCATTC GAATGC(1/-1) BspLU11I 882 ACATGT A^CATGT BstAPI 1512 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 807 GCTCAGC GC^T[ACGT]AGC EagI 2006 CGGCCG C^GGCCG EclXI 2006 CGGCCG C^GGCCG Eco52I 2006 CGGCCG C^GGCCG FspI 5425 TGCGCA TGC^GCA HindIII 781 AAGCTT A^AGCTT HpaI 3250 GTTAAC GTT^AAC KasI 1497 GGCGCC G^GCGCC KpnI 2770 GGTACC GGTAC^C NarI 1497 GGCGCC GG^CGCC PmaCI 283 CACGTG CAC^GTG PmlI 283 CACGTG CAC^GTG PshBI 5373 ATTAAT AT^TAAT PvuII 1822 CAGCTG CAG^CTG SacI 805 GAGCTC GAGCT^C SalI 2011 GTCGAC G^TCGAC SapI 1317 GAAGAGC GCTCTTC(1/4) SfoI 1497 GGCGCC GGC^GCC SmaI 18 CCCGGG CCC^GGG XbaI 4644 TCTAGA T^CTAGA XmaCI 18 CCCGGG C^CCGGG XmaI 18 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 625 GACGTC GACGT^C 1735 GACGTC AflII 4868 CTTAAG C^TTAAG 5451 CTTAAG AocI 3565 CCTCAGG CC^T[ACGT]AGG 4199 CCTCAGG AspI 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 231 GACCACGTC BamHI 1078 GGATCC G^GATCC 2287 GGATCC BciVI 278 GTATCC GTATCC(6/5) 1453 GTATCC BclI 4535 TGATCA T^GATCA 4727 TGATCA BfrI 4868 CTTAAG C^TTAAG 5451 CTTAAG BsaBI 1548 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4880 GATAGAAATC BsaWI 3 ACCGGA [AT]^CCGG[AT] 2230 ACCGGA BseRI 3740 CTCCTC GAGGAG(10/8) 5198 GAGGAG BsmBI 183 CGTCTC CGTCTC(1/5) 65 GAGACG BspHI 680 TCATGA T^CATGA 4724 TCATGA BsrBI 857 GAGCGG CCGCTC(-3/-3) 1929 GAGCGG BsrFI 896 ACCGGC [GA]^CCGG[CT] 1631 GCCGGT BssSI 4325 CACGAG CACGAG(-5/-1) 205 CTCGTG Bst1107I 2808 GTATAC GTA^TAC 2991 GTATAC BstXI 372 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2089 CCAGCCCCCTGG BstZ17I 2808 GTATAC GTA^TAC 2991 GTATAC Bsu36I 3565 CCTCAGG CC^T[ACGT]AGG 4199 CCTCAGG Cfr10I 896 ACCGGC [GA]^CCGG[CT] 1631 GCCGGT Eco81I 3565 CCTCAGG CC^T[ACGT]AGG 4199 CCTCAGG FbaI 4535 TGATCA T^GATCA 4727 TGATCA HaeII 1497 GGCGCC [GA]GCGC^[CT] 1588 GGCGCT HgaI 67 GACGC GACGC(5/10) 2394 GACGC MamI 1548 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4880 GATAGAAATC PflFI 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 231 GACCACGTC RcaI 680 TCATGA T^CATGA 4724 TCATGA ScaI 560 AGTACT AGT^ACT 4742 AGTACT SnaBI 3366 TACGTA TAC^GTA 5432 TACGTA SspI 1122 AATATT AAT^ATT 3293 AATATT StuI 705 AGGCCT AGG^CCT 3391 AGGCCT Tth111I 178 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 231 GACCACGTC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI