##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AviII BalI BbeI
BbrPI BbvCI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BstBI Cfr10I ClaI CpoI EaeI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI FspI Hin1I KasI
KspI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 477 GGTACC G^GTACC
AflII 6235 CTTAAG C^TTAAG
AflIII 2180 ACACGT A^C[GA][CT]GT
AocI 1339 CCTGAGG CC^T[ACGT]AGG
ApaI 3264 GGGCCC GGGCC^C
Asp718I 477 GGTACC G^GTACC
AvrII 6220 CCTAGG C^CTAGG
BfrI 6235 CTTAAG C^TTAAG
BlnI 6220 CCTAGG C^CTAGG
BsaAI 2865 CACGTA [CT]AC^GT[GA]
BsaBI 2473 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2373 TCATGA T^CATGA
BsrDI 2644 GCAATG GCAATG(2/0)
BstEII 345 GGTGACC G^GT[ACGT]ACC
BstPI 345 GGTGACC G^GT[ACGT]ACC
Bsu36I 1339 CCTGAGG CC^T[ACGT]AGG
Eco81I 1339 CCTGAGG CC^T[ACGT]AGG
EcoO65I 345 GGTGACC G^GT[ACGT]ACC
EcoT22I 886 ATGCAT ATGCA^T
HgaI 493 GCGTC GACGC(5/10)
HincII 708 GTTAAC GT[CT]^[GA]AC
HindII 708 GTTAAC GT[CT]^[GA]AC
HpaI 708 GTTAAC GTT^AAC
KpnI 477 GGTACC GGTAC^C
MamI 2473 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 833 CAATTG C^AATTG
MunI 833 CAATTG C^AATTG
NdeI 1646 CATATG CA^TATG
NsiI 886 ATGCAT ATGCA^T
PaeR7I 224 CTCGAG C^TCGAG
RcaI 2373 TCATGA T^CATGA
SapI 3610 GAAGAGC GCTCTTC(1/4)
SphI 5056 GCATGC GCATG^C
SspI 5717 AATATT AAT^ATT
SwaI 5548 ATTTAAAT ATTT^AAAT
XbaI 4641 TCTAGA T^CTAGA
XhoI 224 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspI 4196 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5151 GACACTGTC
BcgI 618 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3903 GCATCATTATCG
BciVI 2617 GGATAC GTATCC(6/5)
5515 GTATCC
BglII 3487 AGATCT A^GATCT
4556 AGATCT
BsrBI 5128 CCGCTC CCGCTC(-3/-3)
2980 GAGCGG
BssSI 1962 CACGAG CACGAG(-5/-1)
2741 CACGAG
Bst1107I 2414 GTATAC GTA^TAC
6074 GTATAC
BstZ17I 2414 GTATAC GTA^TAC
6074 GTATAC
DraIII 275 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2044 CACAATGTG
DrdI 1710 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6000 GACTATCCTGTC
PflFI 4196 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5151 GACACTGTC
PflMI 1852 CCATACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4716 CCATCCTGTGG
SacI 2193 GAGCTC GAGCT^C
4596 GAGCTC
SexAI 3078 ACCAGGT A^CC[AT]GGT
4197 ACCAGGT
Tth111I 4196 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5151 GACACTGTC
Van91I 1852 CCATACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4716 CCATCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI
XmnI