################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccII AclI AgeI AhdI AscI AspEI AviII BamHI BcgI BlpI Bpu1102I BsaI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FseI FspI KspI MluI MvnI NaeI NgoMIV NotI NruI PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 253 TCCGGA T^CCGGA AflII 5679 CTTAAG C^TTAAG AflIII 4471 ACACGT A^C[GA][CT]GT Alw44I 1250 GTGCAC G^TGCAC AocI 5132 CCTAAGG CC^T[ACGT]AGG ApaLI 1250 GTGCAC G^TGCAC AspI 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2914 CCTAGG C^CTAGG BbrPI 1327 CACGTG CAC^GTG BbvCI 3669 GCTGAGG CCTCAGC(-5/-2) BfrI 5679 CTTAAG C^TTAAG BglI 1387 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3663 AGATCT A^GATCT BlnI 2914 CCTAGG C^CTAGG BsaAI 1327 CACGTG [CT]AC^GT[GA] BsaWI 253 TCCGGA [AT]^CCGG[AT] BseAI 253 TCCGGA T^CCGGA BspEI 253 TCCGGA T^CCGGA BstBI 1475 TTCGAA TT^CGAA BstXI 3179 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 5132 CCTAAGG CC^T[ACGT]AGG DraI 4506 TTTAAA TTT^AAA Eco81I 5132 CCTAAGG CC^T[ACGT]AGG EcoRV 2040 GATATC GAT^ATC FauI 4584 CCCGC CCCGC(4/6) HpaI 2199 GTTAAC GTT^AAC MroI 253 TCCGGA T^CCGGA NdeI 4493 CATATG CA^TATG NheI 277 GCTAGC G^CTAGC NspV 1475 TTCGAA TT^CGAA PflFI 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1327 CACGTG CAC^GTG PmlI 1327 CACGTG CAC^GTG PshAI 4142 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfuI 1475 TTCGAA TT^CGAA SpeI 52 ACTAGT A^CTAGT SspI 5657 AATATT AAT^ATT Tth111I 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Aor51HI 2090 AGCGCT AGC^GCT 2309 AGCGCT ApaI 3633 GGGCCC GGGCC^C 3674 GGGCCC AseI 451 ATTAAT AT^TAAT 3195 ATTAAT AsnI 451 ATTAAT AT^TAAT 3195 ATTAAT AvaI 1994 CTCGGG C^[CT]CG[GA]G 3458 CTCGGG BbeI 1384 GGCGCC GGCGC^C 1427 GGCGCC BclI 2990 TGATCA T^GATCA 3986 TGATCA BsgI 413 CTGCAC GTGCAG(16/14) 3596 GTGCAG BsmI 1092 GAATGC GAATGC(1/-1) 4625 GCATTC BsoBI 1994 CTCGGG C^[CT]CG[GA]G 3458 CTCGGG DrdI 1156 GACCACATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5259 GACTGTATTGTC Eco47III 2090 AGCGCT AGC^GCT 2309 AGCGCT EcoT22I 2929 ATGCAT ATGCA^T 4496 ATGCAT FbaI 2990 TGATCA T^GATCA 3986 TGATCA HincII 2199 GTTAAC GT[CT]^[GA]AC 2566 GTCAAC HindII 2199 GTTAAC GT[CT]^[GA]AC 2566 GTCAAC KasI 1384 GGCGCC G^GCGCC 1427 GGCGCC MfeI 1411 CAATTG C^AATTG 2267 CAATTG MunI 1411 CAATTG C^AATTG 2267 CAATTG NarI 1384 GGCGCC GG^CGCC 1427 GGCGCC NsiI 2929 ATGCAT ATGCA^T 4496 ATGCAT PflMI 202 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4940 CCAGCACCTGG PshBI 451 ATTAAT AT^TAAT 3195 ATTAAT SexAI 15 ACCAGGT A^CC[AT]GGT 3857 ACCTGGT SfoI 1384 GGCGCC GGC^GCC 1427 GGCGCC SphI 1227 GCATGC GCATG^C 4587 GCATGC Van91I 202 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4940 CCAGCACCTGG XcmI 1025 CCAGCACATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3062 CCATCCCTGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### Acc65I AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I Asp718I AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI