##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
AscI AspEI AviII BamHI BcgI
BlpI Bpu1102I BsaI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI FseI
FspI KspI MluI MvnI NaeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 253 TCCGGA T^CCGGA
AflII 5679 CTTAAG C^TTAAG
AflIII 4471 ACACGT A^C[GA][CT]GT
Alw44I 1250 GTGCAC G^TGCAC
AocI 5132 CCTAAGG CC^T[ACGT]AGG
ApaLI 1250 GTGCAC G^TGCAC
AspI 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2914 CCTAGG C^CTAGG
BbrPI 1327 CACGTG CAC^GTG
BbvCI 3669 GCTGAGG CCTCAGC(-5/-2)
BfrI 5679 CTTAAG C^TTAAG
BglI 1387 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3663 AGATCT A^GATCT
BlnI 2914 CCTAGG C^CTAGG
BsaAI 1327 CACGTG [CT]AC^GT[GA]
BsaWI 253 TCCGGA [AT]^CCGG[AT]
BseAI 253 TCCGGA T^CCGGA
BspEI 253 TCCGGA T^CCGGA
BstBI 1475 TTCGAA TT^CGAA
BstXI 3179 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5132 CCTAAGG CC^T[ACGT]AGG
DraI 4506 TTTAAA TTT^AAA
Eco81I 5132 CCTAAGG CC^T[ACGT]AGG
EcoRV 2040 GATATC GAT^ATC
FauI 4584 CCCGC CCCGC(4/6)
HpaI 2199 GTTAAC GTT^AAC
MroI 253 TCCGGA T^CCGGA
NdeI 4493 CATATG CA^TATG
NheI 277 GCTAGC G^CTAGC
NspV 1475 TTCGAA TT^CGAA
PflFI 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1327 CACGTG CAC^GTG
PmlI 1327 CACGTG CAC^GTG
PshAI 4142 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1475 TTCGAA TT^CGAA
SpeI 52 ACTAGT A^CTAGT
SspI 5657 AATATT AAT^ATT
Tth111I 5625 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 2090 AGCGCT AGC^GCT
2309 AGCGCT
ApaI 3633 GGGCCC GGGCC^C
3674 GGGCCC
AseI 451 ATTAAT AT^TAAT
3195 ATTAAT
AsnI 451 ATTAAT AT^TAAT
3195 ATTAAT
AvaI 1994 CTCGGG C^[CT]CG[GA]G
3458 CTCGGG
BbeI 1384 GGCGCC GGCGC^C
1427 GGCGCC
BclI 2990 TGATCA T^GATCA
3986 TGATCA
BsgI 413 CTGCAC GTGCAG(16/14)
3596 GTGCAG
BsmI 1092 GAATGC GAATGC(1/-1)
4625 GCATTC
BsoBI 1994 CTCGGG C^[CT]CG[GA]G
3458 CTCGGG
DrdI 1156 GACCACATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5259 GACTGTATTGTC
Eco47III 2090 AGCGCT AGC^GCT
2309 AGCGCT
EcoT22I 2929 ATGCAT ATGCA^T
4496 ATGCAT
FbaI 2990 TGATCA T^GATCA
3986 TGATCA
HincII 2199 GTTAAC GT[CT]^[GA]AC
2566 GTCAAC
HindII 2199 GTTAAC GT[CT]^[GA]AC
2566 GTCAAC
KasI 1384 GGCGCC G^GCGCC
1427 GGCGCC
MfeI 1411 CAATTG C^AATTG
2267 CAATTG
MunI 1411 CAATTG C^AATTG
2267 CAATTG
NarI 1384 GGCGCC GG^CGCC
1427 GGCGCC
NsiI 2929 ATGCAT ATGCA^T
4496 ATGCAT
PflMI 202 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4940 CCAGCACCTGG
PshBI 451 ATTAAT AT^TAAT
3195 ATTAAT
SexAI 15 ACCAGGT A^CC[AT]GGT
3857 ACCTGGT
SfoI 1384 GGCGCC GGC^GCC
1427 GGCGCC
SphI 1227 GCATGC GCATG^C
4587 GCATGC
Van91I 202 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4940 CCAGCACCTGG
XcmI 1025 CCAGCACATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3062 CCATCCCTGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I Asp718I AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI