################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII AccIII AgeI Alw44I ApaLI AscI AspI AviII BalI BcgI BclI BsaBI BseAI BspEI BsrBI Bst1107I BstZ17I CpoI FbaI FseI FspI HindIII MamI MluI MluNI MroI MscI NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PvuI RsrII SalI SexAI SfiI SpeI SphI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflII 1531 CTTAAG C^TTAAG Aor51HI 3547 AGCGCT AGC^GCT Asp700I 1832 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4614 CCTAGG C^CTAGG BbrPI 5493 CACGTG CAC^GTG BfrI 1531 CTTAAG C^TTAAG BglI 3633 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3969 AGATCT A^GATCT BlnI 4614 CCTAGG C^CTAGG BlpI 1967 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1967 GCTCAGC GC^T[ACGT]AGC BsaWI 1137 TCCGGT [AT]^CCGG[AT] BsiWI 5620 CGTACG C^GTACG BspDI 604 ATCGAT AT^CGAT BspHI 5207 TCATGA T^CATGA BspLU11I 5312 ACATGT A^CATGT BssSI 3119 CACGAG CACGAG(-5/-1) BstBI 1829 TTCGAA TT^CGAA BstEII 5081 GGTTACC G^GT[ACGT]ACC BstPI 5081 GGTTACC G^GT[ACGT]ACC CelII 1967 GCTCAGC GC^T[ACGT]AGC ClaI 604 ATCGAT AT^CGAT DraIII 4030 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1194 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3547 AGCGCT AGC^GCT EcoO65I 5081 GGTTACC G^GT[ACGT]ACC EcoRI 4514 GAATTC G^AATTC EcoRV 3462 GATATC GAT^ATC EcoT22I 782 ATGCAT ATGCA^T MfeI 5739 CAATTG C^AATTG MunI 5739 CAATTG C^AATTG NsiI 782 ATGCAT ATGCA^T NspV 1829 TTCGAA TT^CGAA PmaCI 5493 CACGTG CAC^GTG PmlI 5493 CACGTG CAC^GTG RcaI 5207 TCATGA T^CATGA SbfI 738 CCTGCAGG CCTGCA^GG ScaI 5267 AGTACT AGT^ACT SfuI 1829 TTCGAA TT^CGAA SgrAI 3999 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 4788 TACGTA TAC^GTA Sse8387I 738 CCTGCAGG CCTGCA^GG XbaI 4372 TCTAGA T^CTAGA XmnI 1832 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 88 GGTACC G^GTACC 3225 GGTACC AccI 487 GTCTAC GT^[AC][GT]AC 1988 GTCTAC AclI 801 AACGTT AA^CGTT 5127 AACGTT AhdI 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5183 GACAAAATGTC Asp718I 88 GGTACC G^GTACC 3225 GGTACC AspEI 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5183 GACAAAATGTC BamHI 528 GGATCC G^GATCC 2024 GGATCC BbvCI 807 CCTCAGC CCTCAGC(-5/-2) 166 GCTGAGG BciVI 1278 GTATCC GTATCC(6/5) 4610 GTATCC BsaI 1463 GAGACC GGTCTC(1/5) 2530 GGTCTC BsmI 2993 GCATTC GAATGC(1/-1) 4961 GCATTC Eam1105I 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5183 GACAAAATGTC EcoNI 2254 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4485 CCTACTGCAGG HpaI 2095 GTTAAC GTT^AAC 5130 GTTAAC KpnI 88 GGTACC GGTAC^C 3225 GGTACC KspI 3691 CCGCGG CCGC^GG 3859 CCGCGG NdeI 587 CATATG CA^TATG 1760 CATATG PshAI 1981 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2524 GACGCCGGTC Psp1406I 801 AACGTT AA^CGTT 5127 AACGTT SacII 3691 CCGCGG CCGC^GG 3859 CCGCGG SapI 376 GAAGAGC GCTCTTC(1/4) 4846 GCTCTTC SspI 4904 AATATT AAT^ATT 5807 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI