##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AccIII AgeI Alw44I ApaLI
AscI AspI AviII BalI BcgI
BclI BsaBI BseAI BspEI BsrBI
Bst1107I BstZ17I CpoI FbaI FseI
FspI HindIII MamI MluI MluNI
MroI MscI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PvuI RsrII SalI SexAI SfiI
SpeI SphI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 1531 CTTAAG C^TTAAG
Aor51HI 3547 AGCGCT AGC^GCT
Asp700I 1832 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4614 CCTAGG C^CTAGG
BbrPI 5493 CACGTG CAC^GTG
BfrI 1531 CTTAAG C^TTAAG
BglI 3633 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3969 AGATCT A^GATCT
BlnI 4614 CCTAGG C^CTAGG
BlpI 1967 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1967 GCTCAGC GC^T[ACGT]AGC
BsaWI 1137 TCCGGT [AT]^CCGG[AT]
BsiWI 5620 CGTACG C^GTACG
BspDI 604 ATCGAT AT^CGAT
BspHI 5207 TCATGA T^CATGA
BspLU11I 5312 ACATGT A^CATGT
BssSI 3119 CACGAG CACGAG(-5/-1)
BstBI 1829 TTCGAA TT^CGAA
BstEII 5081 GGTTACC G^GT[ACGT]ACC
BstPI 5081 GGTTACC G^GT[ACGT]ACC
CelII 1967 GCTCAGC GC^T[ACGT]AGC
ClaI 604 ATCGAT AT^CGAT
DraIII 4030 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1194 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3547 AGCGCT AGC^GCT
EcoO65I 5081 GGTTACC G^GT[ACGT]ACC
EcoRI 4514 GAATTC G^AATTC
EcoRV 3462 GATATC GAT^ATC
EcoT22I 782 ATGCAT ATGCA^T
MfeI 5739 CAATTG C^AATTG
MunI 5739 CAATTG C^AATTG
NsiI 782 ATGCAT ATGCA^T
NspV 1829 TTCGAA TT^CGAA
PmaCI 5493 CACGTG CAC^GTG
PmlI 5493 CACGTG CAC^GTG
RcaI 5207 TCATGA T^CATGA
SbfI 738 CCTGCAGG CCTGCA^GG
ScaI 5267 AGTACT AGT^ACT
SfuI 1829 TTCGAA TT^CGAA
SgrAI 3999 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 4788 TACGTA TAC^GTA
Sse8387I 738 CCTGCAGG CCTGCA^GG
XbaI 4372 TCTAGA T^CTAGA
XmnI 1832 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 88 GGTACC G^GTACC
3225 GGTACC
AccI 487 GTCTAC GT^[AC][GT]AC
1988 GTCTAC
AclI 801 AACGTT AA^CGTT
5127 AACGTT
AhdI 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5183 GACAAAATGTC
Asp718I 88 GGTACC G^GTACC
3225 GGTACC
AspEI 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5183 GACAAAATGTC
BamHI 528 GGATCC G^GATCC
2024 GGATCC
BbvCI 807 CCTCAGC CCTCAGC(-5/-2)
166 GCTGAGG
BciVI 1278 GTATCC GTATCC(6/5)
4610 GTATCC
BsaI 1463 GAGACC GGTCTC(1/5)
2530 GGTCTC
BsmI 2993 GCATTC GAATGC(1/-1)
4961 GCATTC
Eam1105I 1300 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5183 GACAAAATGTC
EcoNI 2254 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4485 CCTACTGCAGG
HpaI 2095 GTTAAC GTT^AAC
5130 GTTAAC
KpnI 88 GGTACC GGTAC^C
3225 GGTACC
KspI 3691 CCGCGG CCGC^GG
3859 CCGCGG
NdeI 587 CATATG CA^TATG
1760 CATATG
PshAI 1981 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2524 GACGCCGGTC
Psp1406I 801 AACGTT AA^CGTT
5127 AACGTT
SacII 3691 CCGCGG CCGC^GG
3859 CCGCGG
SapI 376 GAAGAGC GCTCTTC(1/4)
4846 GCTCTTC
SspI 4904 AATATT AAT^ATT
5807 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AseI AsnI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrFI
BsrGI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI