##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI ApaI AscI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BcgI BcnI
BlnI BsaHI BseAI BsiEI BsiWI
BsoBI BspEI BssHII BstBI BstUI
CfoI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MluI MroI MvnI NarI
NciI NcoI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 1423 CCTCAGG CC^T[ACGT]AGG
AseI 5681 ATTAAT AT^TAAT
AsnI 5681 ATTAAT AT^TAAT
BbrPI 1980 CACGTG CAC^GTG
BbvCI 3822 CCTCAGC CCTCAGC(-5/-2)
BclI 1021 TGATCA T^GATCA
BglI 4319 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3602 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3602 GCTGAGC GC^T[ACGT]AGC
BsaI 2912 GAGACC GGTCTC(1/5)
BsaWI 409 ACCGGA [AT]^CCGG[AT]
BspDI 1718 ATCGAT AT^CGAT
BspHI 2959 TCATGA T^CATGA
BspMI 4 ACCTGC ACCTGC(4/8)
BsrFI 774 GCCGGC [GA]^CCGG[CT]
Bst1107I 2067 GTATAC GTA^TAC
BstEII 514 GGTAACC G^GT[ACGT]ACC
BstPI 514 GGTAACC G^GT[ACGT]ACC
BstXI 588 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2067 GTATAC GTA^TAC
Bsu36I 1423 CCTCAGG CC^T[ACGT]AGG
CelII 3602 GCTGAGC GC^T[ACGT]AGC
Cfr10I 774 GCCGGC [GA]^CCGG[CT]
ClaI 1718 ATCGAT AT^CGAT
Eco81I 1423 CCTCAGG CC^T[ACGT]AGG
EcoO65I 514 GGTAACC G^GT[ACGT]ACC
EcoRI 2369 GAATTC G^AATTC
FauI 3712 GCGGG CCCGC(4/6)
FbaI 1021 TGATCA T^GATCA
MfeI 3015 CAATTG C^AATTG
MunI 3015 CAATTG C^AATTG
NaeI 774 GCCGGC GCC^GGC
NgoMIV 774 GCCGGC G^CCGGC
PflMI 558 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1980 CACGTG CAC^GTG
PmlI 1980 CACGTG CAC^GTG
PshAI 4056 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5681 ATTAAT AT^TAAT
RcaI 2959 TCATGA T^CATGA
SphI 4882 GCATGC GCATG^C
SwaI 5228 ATTTAAAT ATTT^AAAT
Van91I 558 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2067 GTATAC GT^[AC][GT]AC
3051 GTAGAC
AflII 2923 CTTAAG C^TTAAG
3077 CTTAAG
AhdI 1826 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACCCTGGGTC
Alw44I 943 GTGCAC G^TGCAC
3864 GTGCAC
ApaLI 943 GTGCAC G^TGCAC
3864 GTGCAC
Asp700I 179 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5194 GAAGATATTC
AspEI 1826 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACCCTGGGTC
BfrI 2923 CTTAAG C^TTAAG
3077 CTTAAG
BsaAI 1980 CACGTG [CT]AC^GT[GA]
3246 CACGTA
BsaBI 176 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2845 GATAAAGATC
BsmBI 1872 CGTCTC CGTCTC(1/5)
3905 GAGACG
BsmI 671 GAATGC GAATGC(1/-1)
4093 GCATTC
BsrBI 3710 GAGCGG CCGCTC(-3/-3)
4157 GAGCGG
BsrDI 4014 CATTGC GCAATG(2/0)
3935 GCAATG
BssSI 2496 CACGAG CACGAG(-5/-1)
3293 CACGAG
Eam1105I 1826 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACCCTGGGTC
EcoNI 2338 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5308 CCTTTTAAAGG
EcoT22I 849 ATGCAT ATGCA^T
2456 ATGCAT
HincII 4535 GTCAAC GT[CT]^[GA]AC
5180 GTTGAC
HindII 4535 GTCAAC GT[CT]^[GA]AC
5180 GTTGAC
HindIII 1782 AAGCTT A^AGCTT
3481 AAGCTT
MamI 176 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2845 GATAAAGATC
NheI 764 GCTAGC G^CTAGC
964 GCTAGC
NsiI 849 ATGCAT ATGCA^T
2456 ATGCAT
SexAI 2979 ACCAGGT A^CC[AT]GGT
4243 ACCAGGT
SspI 2171 AATATT AAT^ATT
5573 AATATT
XbaI 2197 TCTAGA T^CTAGA
4268 TCTAGA
XmnI 179 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5194 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvI BciVI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII