################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccI AccIII AclI AgeI AscI AviII AvrII BbeI BbsI BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRI EcoRV EcoT22I FseI FspI KasI MamI MluI MroI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1804 GGTACC G^GTACC AcyI 20 GGCGTC G[GA]^CG[CT]C AflII 2600 CTTAAG C^TTAAG AflIII 1962 ACGTGT A^C[GA][CT]GT AseI 3861 ATTAAT AT^TAAT AsnI 3861 ATTAAT AT^TAAT Asp700I 3236 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1804 GGTACC G^GTACC AspI 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1125 CACGTG CAC^GTG BbvCI 3587 GCTGAGG CCTCAGC(-5/-2) BcgI 2528 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2600 CTTAAG C^TTAAG BglI 66 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1193 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1193 GCTGAGC GC^T[ACGT]AGC BsaHI 20 GGCGTC G[GA]^CG[CT]C BsaWI 1801 TCCGGT [AT]^CCGG[AT] BsmI 3802 GAATGC GAATGC(1/-1) Bsp1407I 3852 TGTACA T^GTACA BsrFI 56 GCCGGC [GA]^CCGG[CT] BsrGI 3852 TGTACA T^GTACA BstEII 2904 GGTCACC G^GT[ACGT]ACC BstPI 2904 GGTCACC G^GT[ACGT]ACC CelII 1193 GCTGAGC GC^T[ACGT]AGC Cfr10I 56 GCCGGC [GA]^CCGG[CT] DraIII 888 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 2904 GGTCACC G^GT[ACGT]ACC Hin1I 20 GGCGTC G[GA]^CG[CT]C HpaI 2563 GTTAAC GTT^AAC KpnI 1804 GGTACC GGTAC^C MfeI 299 CAATTG C^AATTG MunI 299 CAATTG C^AATTG NaeI 56 GCCGGC GCC^GGC NgoMIV 56 GCCGGC G^CCGGC PflFI 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1125 CACGTG CAC^GTG PmlI 1125 CACGTG CAC^GTG PshAI 2749 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3861 ATTAAT AT^TAAT SacI 2473 GAGCTC GAGCT^C SfiI 65 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1380 CCCGGG CCC^GGG SpeI 2077 ACTAGT A^CTAGT SspBI 3852 TGTACA T^GTACA StuI 2268 AGGCCT AGG^CCT Tth111I 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3015 CCACTGGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1380 CCCGGG C^CCGGG XmaI 1380 CCCGGG C^CCGGG XmnI 3236 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 520 AAATTC [GA]^AATT[CT] 3695 AAATTT Alw44I 1611 GTGCAC G^TGCAC 2452 GTGCAC AocI 1716 CCTCAGG CC^T[ACGT]AGG 2899 CCTCAGG Aor51HI 39 AGCGCT AGC^GCT 3145 AGCGCT ApaLI 1611 GTGCAC G^TGCAC 2452 GTGCAC ApoI 520 AAATTC [GA]^AATT[CT] 3695 AAATTT AvaI 1380 CCCGGG C^[CT]CG[GA]G 1809 CCCGAG BamHI 1594 GGATCC G^GATCC 3657 GGATCC BclI 2334 TGATCA T^GATCA 2711 TGATCA BsaAI 1125 CACGTG [CT]AC^GT[GA] 1571 TACGTG BsiEI 79 CGGCCG CG[GA][CT]^CG 173 CGGCCG BsoBI 1380 CCCGGG C^[CT]CG[GA]G 1809 CCCGAG BsrBI 3129 CCGCTC CCGCTC(-3/-3) 3055 GAGCGG Bsu36I 1716 CCTCAGG CC^T[ACGT]AGG 2899 CCTCAGG DraI 3554 TTTAAA TTT^AAA 3690 TTTAAA EagI 79 CGGCCG C^GGCCG 173 CGGCCG EarI 3153 CTCTTC CTCTTC(1/4) 3792 CTCTTC EclXI 79 CGGCCG C^GGCCG 173 CGGCCG Eco47III 39 AGCGCT AGC^GCT 3145 AGCGCT Eco52I 79 CGGCCG C^GGCCG 173 CGGCCG Eco81I 1716 CCTCAGG CC^T[ACGT]AGG 2899 CCTCAGG EcoNI 200 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1655 CCTGGTGGAGG FbaI 2334 TGATCA T^GATCA 2711 TGATCA HindIII 490 AAGCTT A^AGCTT 3215 AAGCTT Ksp632I 3153 CTCTTC CTCTTC(1/4) 3792 CTCTTC KspI 76 CCGCGG CCGC^GG 3332 CCGCGG PflMI 794 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1367 CCAGACCCTGG PpuMI 1564 GGGACCC [GA]G^G[AT]CC[CT] 1813 AGGACCT SacII 76 CCGCGG CCGC^GG 3332 CCGCGG ScaI 628 AGTACT AGT^ACT 3411 AGTACT SspI 1845 AATATT AAT^ATT 3771 AATATT Van91I 794 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1367 CCAGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AfaI AhdI AluI AlwI AlwNI ApaI AspEI AspHI AvaII BalI BanI BanII BbvI BciVI BcnI BfaI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII