##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AscI        AviII       AvrII       BbeI        BbsI        
BlnI        BpuAI       BsaBI       BsaI        BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        EcoRI       EcoRV       
EcoT22I     FseI        FspI        KasI        MamI        
MluI        MroI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       1804  GGTACC               G^GTACC
AcyI           20  GGCGTC               G[GA]^CG[CT]C
AflII        2600  CTTAAG               C^TTAAG
AflIII       1962  ACGTGT               A^C[GA][CT]GT
AseI         3861  ATTAAT               AT^TAAT
AsnI         3861  ATTAAT               AT^TAAT
Asp700I      3236  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1804  GGTACC               G^GTACC
AspI          291  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1125  CACGTG               CAC^GTG
BbvCI        3587  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2528  CGAGTGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2600  CTTAAG               C^TTAAG
BglI           66  GCCGAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1193  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1193  GCTGAGC              GC^T[ACGT]AGC
BsaHI          20  GGCGTC               G[GA]^CG[CT]C
BsaWI        1801  TCCGGT               [AT]^CCGG[AT]
BsmI         3802  GAATGC               GAATGC(1/-1)
Bsp1407I     3852  TGTACA               T^GTACA
BsrFI          56  GCCGGC               [GA]^CCGG[CT]
BsrGI        3852  TGTACA               T^GTACA
BstEII       2904  GGTCACC              G^GT[ACGT]ACC
BstPI        2904  GGTCACC              G^GT[ACGT]ACC
CelII        1193  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         56  GCCGGC               [GA]^CCGG[CT]
DraIII        888  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2904  GGTCACC              G^GT[ACGT]ACC
Hin1I          20  GGCGTC               G[GA]^CG[CT]C
HpaI         2563  GTTAAC               GTT^AAC
KpnI         1804  GGTACC               GGTAC^C
MfeI          299  CAATTG               C^AATTG
MunI          299  CAATTG               C^AATTG
NaeI           56  GCCGGC               GCC^GGC
NgoMIV         56  GCCGGC               G^CCGGC
PflFI         291  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1125  CACGTG               CAC^GTG
PmlI         1125  CACGTG               CAC^GTG
PshAI        2749  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3861  ATTAAT               AT^TAAT
SacI         2473  GAGCTC               GAGCT^C
SfiI           65  GGCCGAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1380  CCCGGG               CCC^GGG
SpeI         2077  ACTAGT               A^CTAGT
SspBI        3852  TGTACA               T^GTACA
StuI         2268  AGGCCT               AGG^CCT
Tth111I       291  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         3015  CCACTGGGCCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1380  CCCGGG               C^CCGGG
XmaI         1380  CCCGGG               C^CCGGG
XmnI         3236  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcsI          520  AAATTC               [GA]^AATT[CT]
             3695  AAATTT              
Alw44I       1611  GTGCAC               G^TGCAC
             2452  GTGCAC              
AocI         1716  CCTCAGG              CC^T[ACGT]AGG
             2899  CCTCAGG             
Aor51HI        39  AGCGCT               AGC^GCT
             3145  AGCGCT              
ApaLI        1611  GTGCAC               G^TGCAC
             2452  GTGCAC              
ApoI          520  AAATTC               [GA]^AATT[CT]
             3695  AAATTT              
AvaI         1380  CCCGGG               C^[CT]CG[GA]G
             1809  CCCGAG              
BamHI        1594  GGATCC               G^GATCC
             3657  GGATCC              
BclI         2334  TGATCA               T^GATCA
             2711  TGATCA              
BsaAI        1125  CACGTG               [CT]AC^GT[GA]
             1571  TACGTG              
BsiEI          79  CGGCCG               CG[GA][CT]^CG
              173  CGGCCG              
BsoBI        1380  CCCGGG               C^[CT]CG[GA]G
             1809  CCCGAG              
BsrBI        3129  CCGCTC               CCGCTC(-3/-3)
             3055  GAGCGG              
Bsu36I       1716  CCTCAGG              CC^T[ACGT]AGG
             2899  CCTCAGG             
DraI         3554  TTTAAA               TTT^AAA
             3690  TTTAAA              
EagI           79  CGGCCG               C^GGCCG
              173  CGGCCG              
EarI         3153  CTCTTC               CTCTTC(1/4)
             3792  CTCTTC              
EclXI          79  CGGCCG               C^GGCCG
              173  CGGCCG              
Eco47III       39  AGCGCT               AGC^GCT
             3145  AGCGCT              
Eco52I         79  CGGCCG               C^GGCCG
              173  CGGCCG              
Eco81I       1716  CCTCAGG              CC^T[ACGT]AGG
             2899  CCTCAGG             
EcoNI         200  CCTGCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1655  CCTGGTGGAGG         
FbaI         2334  TGATCA               T^GATCA
             2711  TGATCA              
HindIII       490  AAGCTT               A^AGCTT
             3215  AAGCTT              
Ksp632I      3153  CTCTTC               CTCTTC(1/4)
             3792  CTCTTC              
KspI           76  CCGCGG               CCGC^GG
             3332  CCGCGG              
PflMI         794  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1367  CCAGACCCTGG         
PpuMI        1564  GGGACCC              [GA]G^G[AT]CC[CT]
             1813  AGGACCT             
SacII          76  CCGCGG               CCGC^GG
             3332  CCGCGG              
ScaI          628  AGTACT               AGT^ACT
             3411  AGTACT              
SspI         1845  AATATT               AAT^ATT
             3771  AATATT              
Van91I        794  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1367  CCAGACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApaI        AspEI       AspHI       
AvaII       BalI        BanI        BanII       BbvI        
BciVI       BcnI        BfaI        BglII       BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eam1105I    
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII