##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AscI AviII AvrII BbeI BbsI
BlnI BpuAI BsaBI BsaI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EcoRI EcoRV
EcoT22I FseI FspI KasI MamI
MluI MroI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1804 GGTACC G^GTACC
AcyI 20 GGCGTC G[GA]^CG[CT]C
AflII 2600 CTTAAG C^TTAAG
AflIII 1962 ACGTGT A^C[GA][CT]GT
AseI 3861 ATTAAT AT^TAAT
AsnI 3861 ATTAAT AT^TAAT
Asp700I 3236 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1804 GGTACC G^GTACC
AspI 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1125 CACGTG CAC^GTG
BbvCI 3587 GCTGAGG CCTCAGC(-5/-2)
BcgI 2528 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2600 CTTAAG C^TTAAG
BglI 66 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1193 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1193 GCTGAGC GC^T[ACGT]AGC
BsaHI 20 GGCGTC G[GA]^CG[CT]C
BsaWI 1801 TCCGGT [AT]^CCGG[AT]
BsmI 3802 GAATGC GAATGC(1/-1)
Bsp1407I 3852 TGTACA T^GTACA
BsrFI 56 GCCGGC [GA]^CCGG[CT]
BsrGI 3852 TGTACA T^GTACA
BstEII 2904 GGTCACC G^GT[ACGT]ACC
BstPI 2904 GGTCACC G^GT[ACGT]ACC
CelII 1193 GCTGAGC GC^T[ACGT]AGC
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
DraIII 888 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2904 GGTCACC G^GT[ACGT]ACC
Hin1I 20 GGCGTC G[GA]^CG[CT]C
HpaI 2563 GTTAAC GTT^AAC
KpnI 1804 GGTACC GGTAC^C
MfeI 299 CAATTG C^AATTG
MunI 299 CAATTG C^AATTG
NaeI 56 GCCGGC GCC^GGC
NgoMIV 56 GCCGGC G^CCGGC
PflFI 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1125 CACGTG CAC^GTG
PmlI 1125 CACGTG CAC^GTG
PshAI 2749 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3861 ATTAAT AT^TAAT
SacI 2473 GAGCTC GAGCT^C
SfiI 65 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1380 CCCGGG CCC^GGG
SpeI 2077 ACTAGT A^CTAGT
SspBI 3852 TGTACA T^GTACA
StuI 2268 AGGCCT AGG^CCT
Tth111I 291 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3015 CCACTGGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1380 CCCGGG C^CCGGG
XmaI 1380 CCCGGG C^CCGGG
XmnI 3236 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 520 AAATTC [GA]^AATT[CT]
3695 AAATTT
Alw44I 1611 GTGCAC G^TGCAC
2452 GTGCAC
AocI 1716 CCTCAGG CC^T[ACGT]AGG
2899 CCTCAGG
Aor51HI 39 AGCGCT AGC^GCT
3145 AGCGCT
ApaLI 1611 GTGCAC G^TGCAC
2452 GTGCAC
ApoI 520 AAATTC [GA]^AATT[CT]
3695 AAATTT
AvaI 1380 CCCGGG C^[CT]CG[GA]G
1809 CCCGAG
BamHI 1594 GGATCC G^GATCC
3657 GGATCC
BclI 2334 TGATCA T^GATCA
2711 TGATCA
BsaAI 1125 CACGTG [CT]AC^GT[GA]
1571 TACGTG
BsiEI 79 CGGCCG CG[GA][CT]^CG
173 CGGCCG
BsoBI 1380 CCCGGG C^[CT]CG[GA]G
1809 CCCGAG
BsrBI 3129 CCGCTC CCGCTC(-3/-3)
3055 GAGCGG
Bsu36I 1716 CCTCAGG CC^T[ACGT]AGG
2899 CCTCAGG
DraI 3554 TTTAAA TTT^AAA
3690 TTTAAA
EagI 79 CGGCCG C^GGCCG
173 CGGCCG
EarI 3153 CTCTTC CTCTTC(1/4)
3792 CTCTTC
EclXI 79 CGGCCG C^GGCCG
173 CGGCCG
Eco47III 39 AGCGCT AGC^GCT
3145 AGCGCT
Eco52I 79 CGGCCG C^GGCCG
173 CGGCCG
Eco81I 1716 CCTCAGG CC^T[ACGT]AGG
2899 CCTCAGG
EcoNI 200 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1655 CCTGGTGGAGG
FbaI 2334 TGATCA T^GATCA
2711 TGATCA
HindIII 490 AAGCTT A^AGCTT
3215 AAGCTT
Ksp632I 3153 CTCTTC CTCTTC(1/4)
3792 CTCTTC
KspI 76 CCGCGG CCGC^GG
3332 CCGCGG
PflMI 794 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCAGACCCTGG
PpuMI 1564 GGGACCC [GA]G^G[AT]CC[CT]
1813 AGGACCT
SacII 76 CCGCGG CCGC^GG
3332 CCGCGG
ScaI 628 AGTACT AGT^ACT
3411 AGTACT
SspI 1845 AATATT AAT^ATT
3771 AATATT
Van91I 794 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI AlwNI ApaI AspEI AspHI
AvaII BalI BanI BanII BbvI
BciVI BcnI BfaI BglII BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII