##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AscI        
AseI        AsnI        AviII       AvrII       BalI        
BbrPI       BclI        BfrI        BglII       BlnI        
BsaBI       BsrBI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     CpoI        EcoT22I     FbaI        
FspI        KspI        MamI        MfeI        MluNI       
MscI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccIII        933  TCCGGA               T^CCGGA
ApaI         3303  GGGCCC               GGGCC^C
AspI         2780  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         184  GGATCC               G^GATCC
BglI         2111  GCCGGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3218  CACGTA               [CT]AC^GT[GA]
BsaI         1584  GGTCTC               GGTCTC(1/5)
BseAI         933  TCCGGA               T^CCGGA
BsiWI        3220  CGTACG               C^GTACG
BspDI         494  ATCGAT               AT^CGAT
BspEI         933  TCCGGA               T^CCGGA
BspHI        3583  TCATGA               T^CATGA
BstXI        1422  CCACACCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          494  ATCGAT               AT^CGAT
EcoRI         892  GAATTC               G^AATTC
EcoRV        2529  GATATC               GAT^ATC
FseI         2493  GGCCGGCC             GGCCGG^CC
HindIII      2963  AAGCTT               A^AGCTT
MluI         3394  ACGCGT               A^CGCGT
MroI          933  TCCGGA               T^CCGGA
NcoI         1125  CCATGG               C^CATGG
PaeR7I       1156  CTCGAG               C^TCGAG
PflFI        2780  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2096  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI         3583  TCATGA               T^CATGA
SmaI         2093  CCCGGG               CCC^GGG
SwaI         3687  ATTTAAAT             ATTT^AAAT
Tth111I      2780  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2508  TCTAGA               T^CTAGA
XhoI         1156  CTCGAG               C^TCGAG
XmaCI        2093  CCCGGG               C^CCGGG
XmaI         2093  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   53 enzymes) #
###################################

AccI         3254  GTCTAC               GT^[AC][GT]AC
             4945  GTAGAC              
AcsI          892  GAATTC               [GA]^AATT[CT]
             2726  AAATTT              
AcyI         1268  GGCGCC               G[GA]^CG[CT]C
             1622  GGCGCC              
AhdI         1133  GACTGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1217  GACTCGCCGTC         
Alw44I       3172  GTGCAC               G^TGCAC
             3501  GTGCAC              
Aor51HI       807  AGCGCT               AGC^GCT
             1683  AGCGCT              
ApaLI        3172  GTGCAC               G^TGCAC
             3501  GTGCAC              
ApoI          892  GAATTC               [GA]^AATT[CT]
             2726  AAATTT              
Asp700I      3476  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4235  GAAGTCCTTC          
AspEI        1133  GACTGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1217  GACTCGCCGTC         
AvaII        2097  GGACC                G^G[AT]CC
             3611  GGACC               
BbeI         1268  GGCGCC               GGCGC^C
             1622  GGCGCC              
BbvCI        2212  CCTCAGC              CCTCAGC(-5/-2)
             1211  GCTGAGG             
BciVI        4574  GGATAC               GTATCC(6/5)
             4486  GTATCC              
BlpI          275  GCTGAGC              GC^T[ACGT]AGC
             1278  GCTCAGC             
Bpu1102I      275  GCTGAGC              GC^T[ACGT]AGC
             1278  GCTCAGC             
BsaHI        1268  GGCGCC               G[GA]^CG[CT]C
             1622  GGCGCC              
BsaWI         933  TCCGGA               [AT]^CCGG[AT]
             1070  ACCGGA              
BsiEI        1642  CGGCCG               CG[GA][CT]^CG
             2496  CGGCCG              
BsmI          400  GAATGC               GAATGC(1/-1)
             3803  GCATTC              
Bsp1407I     3063  TGTACA               T^GTACA
             4403  TGTACA              
BsrDI        3112  CATTGC               GCAATG(2/0)
             1110  GCAATG              
BsrFI        2111  GCCGGC               [GA]^CCGG[CT]
             2494  GCCGGC              
BsrGI        3063  TGTACA               T^GTACA
             4403  TGTACA              
BssSI         982  CACGAG               CACGAG(-5/-1)
             2102  CTCGTG              
CelII         275  GCTGAGC              GC^T[ACGT]AGC
             1278  GCTCAGC             
Cfr10I       2111  GCCGGC               [GA]^CCGG[CT]
             2494  GCCGGC              
DraI         3688  TTTAAA               TTT^AAA
             4550  TTTAAA              
DraII        2096  GGGACCC              [GA]G^G[ACGT]CC[CT]
             3302  AGGGCCC             
DraIII       2296  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4091  CACATTGTG           
DrdI          350  GACCCCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              923  GACCTGCGCGTC        
EagI         1642  CGGCCG               C^GGCCG
             2496  CGGCCG              
Eam1105I     1133  GACTGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1217  GACTCGCCGTC         
EclXI        1642  CGGCCG               C^GGCCG
             2496  CGGCCG              
Eco47III      807  AGCGCT               AGC^GCT
             1683  AGCGCT              
Eco52I       1642  CGGCCG               C^GGCCG
             2496  CGGCCG              
EcoO109I     2096  GGGACCC              [GA]G^G[ACGT]CC[CT]
             3302  AGGGCCC             
Hin1I        1268  GGCGCC               G[GA]^CG[CT]C
             1622  GGCGCC              
HpaI         3160  GTTAAC               GTT^AAC
             4758  GTTAAC              
KasI         1268  GGCGCC               G^GCGCC
             1622  GGCGCC              
NaeI         2111  GCCGGC               GCC^GGC
             2494  GCCGGC              
NarI         1268  GGCGCC               GG^CGCC
             1622  GGCGCC              
NgoMIV       2111  GCCGGC               G^CCGGC
             2494  GCCGGC              
NheI         2053  GCTAGC               G^CTAGC
             2444  GCTAGC              
PflMI         106  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              723  CCAAAATGTGG         
SacI         1159  GAGCTC               GAGCT^C
             2579  GAGCTC              
ScaI         1991  AGTACT               AGT^ACT
             3902  AGTACT              
SfoI         1268  GGCGCC               GGC^GCC
             1622  GGCGCC              
SphI          906  GCATGC               GCATG^C
             1296  GCATGC              
SspBI        3063  TGTACA               T^GTACA
             4403  TGTACA              
Van91I        106  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              723  CCAAAATGTGG         
XcmI          957  CCACAAGGTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1422  CCACACCCGTGGTGG     
XmnI         3476  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4235  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

Acc65I      AccII       AciI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        Asp718I     
AspHI       AvaI        BanI        BanII       BbsI        
BbvI        BcgI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII