##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AviII AvrII BalI
BbrPI BclI BfrI BglII BlnI
BsaBI BsrBI BssHII Bst1107I BstAPI
BstBI BstZ17I CpoI EcoT22I FbaI
FspI KspI MamI MfeI MluNI
MscI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 933 TCCGGA T^CCGGA
ApaI 3303 GGGCCC GGGCC^C
AspI 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 184 GGATCC G^GATCC
BglI 2111 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3218 CACGTA [CT]AC^GT[GA]
BsaI 1584 GGTCTC GGTCTC(1/5)
BseAI 933 TCCGGA T^CCGGA
BsiWI 3220 CGTACG C^GTACG
BspDI 494 ATCGAT AT^CGAT
BspEI 933 TCCGGA T^CCGGA
BspHI 3583 TCATGA T^CATGA
BstXI 1422 CCACACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 494 ATCGAT AT^CGAT
EcoRI 892 GAATTC G^AATTC
EcoRV 2529 GATATC GAT^ATC
FseI 2493 GGCCGGCC GGCCGG^CC
HindIII 2963 AAGCTT A^AGCTT
MluI 3394 ACGCGT A^CGCGT
MroI 933 TCCGGA T^CCGGA
NcoI 1125 CCATGG C^CATGG
PaeR7I 1156 CTCGAG C^TCGAG
PflFI 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2096 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 3583 TCATGA T^CATGA
SmaI 2093 CCCGGG CCC^GGG
SwaI 3687 ATTTAAAT ATTT^AAAT
Tth111I 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2508 TCTAGA T^CTAGA
XhoI 1156 CTCGAG C^TCGAG
XmaCI 2093 CCCGGG C^CCGGG
XmaI 2093 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 53 enzymes) #
###################################
AccI 3254 GTCTAC GT^[AC][GT]AC
4945 GTAGAC
AcsI 892 GAATTC [GA]^AATT[CT]
2726 AAATTT
AcyI 1268 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
AhdI 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1217 GACTCGCCGTC
Alw44I 3172 GTGCAC G^TGCAC
3501 GTGCAC
Aor51HI 807 AGCGCT AGC^GCT
1683 AGCGCT
ApaLI 3172 GTGCAC G^TGCAC
3501 GTGCAC
ApoI 892 GAATTC [GA]^AATT[CT]
2726 AAATTT
Asp700I 3476 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4235 GAAGTCCTTC
AspEI 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1217 GACTCGCCGTC
AvaII 2097 GGACC G^G[AT]CC
3611 GGACC
BbeI 1268 GGCGCC GGCGC^C
1622 GGCGCC
BbvCI 2212 CCTCAGC CCTCAGC(-5/-2)
1211 GCTGAGG
BciVI 4574 GGATAC GTATCC(6/5)
4486 GTATCC
BlpI 275 GCTGAGC GC^T[ACGT]AGC
1278 GCTCAGC
Bpu1102I 275 GCTGAGC GC^T[ACGT]AGC
1278 GCTCAGC
BsaHI 1268 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
BsaWI 933 TCCGGA [AT]^CCGG[AT]
1070 ACCGGA
BsiEI 1642 CGGCCG CG[GA][CT]^CG
2496 CGGCCG
BsmI 400 GAATGC GAATGC(1/-1)
3803 GCATTC
Bsp1407I 3063 TGTACA T^GTACA
4403 TGTACA
BsrDI 3112 CATTGC GCAATG(2/0)
1110 GCAATG
BsrFI 2111 GCCGGC [GA]^CCGG[CT]
2494 GCCGGC
BsrGI 3063 TGTACA T^GTACA
4403 TGTACA
BssSI 982 CACGAG CACGAG(-5/-1)
2102 CTCGTG
CelII 275 GCTGAGC GC^T[ACGT]AGC
1278 GCTCAGC
Cfr10I 2111 GCCGGC [GA]^CCGG[CT]
2494 GCCGGC
DraI 3688 TTTAAA TTT^AAA
4550 TTTAAA
DraII 2096 GGGACCC [GA]G^G[ACGT]CC[CT]
3302 AGGGCCC
DraIII 2296 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4091 CACATTGTG
DrdI 350 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
923 GACCTGCGCGTC
EagI 1642 CGGCCG C^GGCCG
2496 CGGCCG
Eam1105I 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1217 GACTCGCCGTC
EclXI 1642 CGGCCG C^GGCCG
2496 CGGCCG
Eco47III 807 AGCGCT AGC^GCT
1683 AGCGCT
Eco52I 1642 CGGCCG C^GGCCG
2496 CGGCCG
EcoO109I 2096 GGGACCC [GA]G^G[ACGT]CC[CT]
3302 AGGGCCC
Hin1I 1268 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
HpaI 3160 GTTAAC GTT^AAC
4758 GTTAAC
KasI 1268 GGCGCC G^GCGCC
1622 GGCGCC
NaeI 2111 GCCGGC GCC^GGC
2494 GCCGGC
NarI 1268 GGCGCC GG^CGCC
1622 GGCGCC
NgoMIV 2111 GCCGGC G^CCGGC
2494 GCCGGC
NheI 2053 GCTAGC G^CTAGC
2444 GCTAGC
PflMI 106 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
723 CCAAAATGTGG
SacI 1159 GAGCTC GAGCT^C
2579 GAGCTC
ScaI 1991 AGTACT AGT^ACT
3902 AGTACT
SfoI 1268 GGCGCC GGC^GCC
1622 GGCGCC
SphI 906 GCATGC GCATG^C
1296 GCATGC
SspBI 3063 TGTACA T^GTACA
4403 TGTACA
Van91I 106 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
723 CCAAAATGTGG
XcmI 957 CCACAAGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1422 CCACACCCGTGGTGG
XmnI 3476 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4235 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflIII
AluI AlwI AlwNI AocI Asp718I
AspHI AvaI BanI BanII BbsI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI Eco81I EcoNI EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII