################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AflII AgeI AscI AseI AsnI AviII AvrII BalI BbrPI BclI BfrI BglII BlnI BsaBI BsrBI BssHII Bst1107I BstAPI BstBI BstZ17I CpoI EcoT22I FbaI FspI KspI MamI MfeI MluNI MscI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 933 TCCGGA T^CCGGA ApaI 3303 GGGCCC GGGCC^C AspI 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 184 GGATCC G^GATCC BglI 2111 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3218 CACGTA [CT]AC^GT[GA] BsaI 1584 GGTCTC GGTCTC(1/5) BseAI 933 TCCGGA T^CCGGA BsiWI 3220 CGTACG C^GTACG BspDI 494 ATCGAT AT^CGAT BspEI 933 TCCGGA T^CCGGA BspHI 3583 TCATGA T^CATGA BstXI 1422 CCACACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 494 ATCGAT AT^CGAT EcoRI 892 GAATTC G^AATTC EcoRV 2529 GATATC GAT^ATC FseI 2493 GGCCGGCC GGCCGG^CC HindIII 2963 AAGCTT A^AGCTT MluI 3394 ACGCGT A^CGCGT MroI 933 TCCGGA T^CCGGA NcoI 1125 CCATGG C^CATGG PaeR7I 1156 CTCGAG C^TCGAG PflFI 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2096 GGGACCC [GA]G^G[AT]CC[CT] RcaI 3583 TCATGA T^CATGA SmaI 2093 CCCGGG CCC^GGG SwaI 3687 ATTTAAAT ATTT^AAAT Tth111I 2780 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2508 TCTAGA T^CTAGA XhoI 1156 CTCGAG C^TCGAG XmaCI 2093 CCCGGG C^CCGGG XmaI 2093 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 53 enzymes) # ################################### AccI 3254 GTCTAC GT^[AC][GT]AC 4945 GTAGAC AcsI 892 GAATTC [GA]^AATT[CT] 2726 AAATTT AcyI 1268 GGCGCC G[GA]^CG[CT]C 1622 GGCGCC AhdI 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1217 GACTCGCCGTC Alw44I 3172 GTGCAC G^TGCAC 3501 GTGCAC Aor51HI 807 AGCGCT AGC^GCT 1683 AGCGCT ApaLI 3172 GTGCAC G^TGCAC 3501 GTGCAC ApoI 892 GAATTC [GA]^AATT[CT] 2726 AAATTT Asp700I 3476 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4235 GAAGTCCTTC AspEI 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1217 GACTCGCCGTC AvaII 2097 GGACC G^G[AT]CC 3611 GGACC BbeI 1268 GGCGCC GGCGC^C 1622 GGCGCC BbvCI 2212 CCTCAGC CCTCAGC(-5/-2) 1211 GCTGAGG BciVI 4574 GGATAC GTATCC(6/5) 4486 GTATCC BlpI 275 GCTGAGC GC^T[ACGT]AGC 1278 GCTCAGC Bpu1102I 275 GCTGAGC GC^T[ACGT]AGC 1278 GCTCAGC BsaHI 1268 GGCGCC G[GA]^CG[CT]C 1622 GGCGCC BsaWI 933 TCCGGA [AT]^CCGG[AT] 1070 ACCGGA BsiEI 1642 CGGCCG CG[GA][CT]^CG 2496 CGGCCG BsmI 400 GAATGC GAATGC(1/-1) 3803 GCATTC Bsp1407I 3063 TGTACA T^GTACA 4403 TGTACA BsrDI 3112 CATTGC GCAATG(2/0) 1110 GCAATG BsrFI 2111 GCCGGC [GA]^CCGG[CT] 2494 GCCGGC BsrGI 3063 TGTACA T^GTACA 4403 TGTACA BssSI 982 CACGAG CACGAG(-5/-1) 2102 CTCGTG CelII 275 GCTGAGC GC^T[ACGT]AGC 1278 GCTCAGC Cfr10I 2111 GCCGGC [GA]^CCGG[CT] 2494 GCCGGC DraI 3688 TTTAAA TTT^AAA 4550 TTTAAA DraII 2096 GGGACCC [GA]G^G[ACGT]CC[CT] 3302 AGGGCCC DraIII 2296 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 4091 CACATTGTG DrdI 350 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 923 GACCTGCGCGTC EagI 1642 CGGCCG C^GGCCG 2496 CGGCCG Eam1105I 1133 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1217 GACTCGCCGTC EclXI 1642 CGGCCG C^GGCCG 2496 CGGCCG Eco47III 807 AGCGCT AGC^GCT 1683 AGCGCT Eco52I 1642 CGGCCG C^GGCCG 2496 CGGCCG EcoO109I 2096 GGGACCC [GA]G^G[ACGT]CC[CT] 3302 AGGGCCC Hin1I 1268 GGCGCC G[GA]^CG[CT]C 1622 GGCGCC HpaI 3160 GTTAAC GTT^AAC 4758 GTTAAC KasI 1268 GGCGCC G^GCGCC 1622 GGCGCC NaeI 2111 GCCGGC GCC^GGC 2494 GCCGGC NarI 1268 GGCGCC GG^CGCC 1622 GGCGCC NgoMIV 2111 GCCGGC G^CCGGC 2494 GCCGGC NheI 2053 GCTAGC G^CTAGC 2444 GCTAGC PflMI 106 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 723 CCAAAATGTGG SacI 1159 GAGCTC GAGCT^C 2579 GAGCTC ScaI 1991 AGTACT AGT^ACT 3902 AGTACT SfoI 1268 GGCGCC GGC^GCC 1622 GGCGCC SphI 906 GCATGC GCATG^C 1296 GCATGC SspBI 3063 TGTACA T^GTACA 4403 TGTACA Van91I 106 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 723 CCAAAATGTGG XcmI 957 CCACAAGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1422 CCACACCCGTGGTGG XmnI 3476 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4235 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### Acc65I AccII AciI AfaI AflIII AluI AlwI AlwNI AocI Asp718I AspHI AvaI BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI Eco81I EcoNI EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII