##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AflII AgeI AhdI AscI AseI
AsnI AspEI AvrII BbeI BcgI
BciVI BfrI BlnI BsiWI BspDI
BstBI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI HindIII HpaI KasI KspI
MfeI MluI MunI NarI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 5358 GACGTC GACGT^C
Acc65I 2815 GGTACC G^GTACC
AclI 2521 AACGTT AA^CGTT
Aor51HI 3241 AGCGCT AGC^GCT
ApaI 1168 GGGCCC GGGCC^C
Asp718I 2815 GGTACC G^GTACC
AspI 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5404 TGCGCA TGC^GCA
BamHI 5279 GGATCC G^GATCC
BlpI 4276 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4276 GCTCAGC GC^T[ACGT]AGC
BsiEI 4055 CGACCG CG[GA][CT]^CG
Bsp1407I 676 TGTACA T^GTACA
BsrGI 676 TGTACA T^GTACA
BssSI 1200 CACGAG CACGAG(-5/-1)
Bst1107I 933 GTATAC GTA^TAC
BstZ17I 933 GTATAC GTA^TAC
CelII 4276 GCTCAGC GC^T[ACGT]AGC
DraI 3891 TTTAAA TTT^AAA
Eco47III 3241 AGCGCT AGC^GCT
FspI 5404 TGCGCA TGC^GCA
KpnI 2815 GGTACC GGTAC^C
NheI 3719 GCTAGC G^CTAGC
PaeR7I 3676 CTCGAG C^TCGAG
PflFI 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4842 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2521 AACGTT AA^CGTT
SacI 1206 GAGCTC GAGCT^C
SapI 720 GAAGAGC GCTCTTC(1/4)
SbfI 5469 CCTGCAGG CCTGCA^GG
SphI 3682 GCATGC GCATG^C
Sse8387I 5469 CCTGCAGG CCTGCA^GG
SspBI 676 TGTACA T^GTACA
StuI 5614 AGGCCT AGG^CCT
Tth111I 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3676 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BglI 2439 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5590 GCCTGGGTGGC
BsaBI 978 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1565 GATGGCCATC
BsrFI 664 GCCGGC [GA]^CCGG[CT]
4747 GCCGGC
BssHII 1536 GCGCGC G^CGCGC
4586 GCGCGC
Cfr10I 664 GCCGGC [GA]^CCGG[CT]
4747 GCCGGC
MamI 978 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1565 GATGGCCATC
NaeI 664 GCCGGC GCC^GGC
4747 GCCGGC
NgoMIV 664 GCCGGC G^CCGGC
4747 GCCGGC
ScaI 3002 AGTACT AGT^ACT
3538 AGTACT
SspI 833 AATATT AAT^ATT
6047 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI