################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AflII AgeI AhdI AscI AseI AsnI AspEI AvrII BbeI BcgI BciVI BfrI BlnI BsiWI BspDI BstBI ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HindIII HpaI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 5358 GACGTC GACGT^C Acc65I 2815 GGTACC G^GTACC AclI 2521 AACGTT AA^CGTT Aor51HI 3241 AGCGCT AGC^GCT ApaI 1168 GGGCCC GGGCC^C Asp718I 2815 GGTACC G^GTACC AspI 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5404 TGCGCA TGC^GCA BamHI 5279 GGATCC G^GATCC BlpI 4276 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4276 GCTCAGC GC^T[ACGT]AGC BsiEI 4055 CGACCG CG[GA][CT]^CG Bsp1407I 676 TGTACA T^GTACA BsrGI 676 TGTACA T^GTACA BssSI 1200 CACGAG CACGAG(-5/-1) Bst1107I 933 GTATAC GTA^TAC BstZ17I 933 GTATAC GTA^TAC CelII 4276 GCTCAGC GC^T[ACGT]AGC DraI 3891 TTTAAA TTT^AAA Eco47III 3241 AGCGCT AGC^GCT FspI 5404 TGCGCA TGC^GCA KpnI 2815 GGTACC GGTAC^C NheI 3719 GCTAGC G^CTAGC PaeR7I 3676 CTCGAG C^TCGAG PflFI 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4842 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2521 AACGTT AA^CGTT SacI 1206 GAGCTC GAGCT^C SapI 720 GAAGAGC GCTCTTC(1/4) SbfI 5469 CCTGCAGG CCTGCA^GG SphI 3682 GCATGC GCATG^C Sse8387I 5469 CCTGCAGG CCTGCA^GG SspBI 676 TGTACA T^GTACA StuI 5614 AGGCCT AGG^CCT Tth111I 3446 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3676 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BglI 2439 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5590 GCCTGGGTGGC BsaBI 978 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1565 GATGGCCATC BsrFI 664 GCCGGC [GA]^CCGG[CT] 4747 GCCGGC BssHII 1536 GCGCGC G^CGCGC 4586 GCGCGC Cfr10I 664 GCCGGC [GA]^CCGG[CT] 4747 GCCGGC MamI 978 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1565 GATGGCCATC NaeI 664 GCCGGC GCC^GGC 4747 GCCGGC NgoMIV 664 GCCGGC G^CCGGC 4747 GCCGGC ScaI 3002 AGTACT AGT^ACT 3538 AGTACT SspI 833 AATATT AAT^ATT 6047 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 157 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI