##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp718I AviII
BbrPI BbvCI BcgI BseAI BsiEI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraII DrdI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
FseI FspI HgaI KpnI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1833 GTCTAC GT^[AC][GT]AC
AcyI 2004 GGCGCC G[GA]^CG[CT]C
AflII 2409 CTTAAG C^TTAAG
Alw44I 1110 GTGCAC G^TGCAC
AocI 2353 CCTGAGG CC^T[ACGT]AGG
ApaLI 1110 GTGCAC G^TGCAC
AspI 508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2004 GGCGCC GGCGC^C
BfrI 2409 CTTAAG C^TTAAG
BglII 5155 AGATCT A^GATCT
BsaAI 5117 TACGTG [CT]AC^GT[GA]
BsaHI 2004 GGCGCC G[GA]^CG[CT]C
BsaWI 292 ACCGGA [AT]^CCGG[AT]
BsmBI 2439 CGTCTC CGTCTC(1/5)
BspMI 2819 GCAGGT ACCTGC(4/8)
BsrBI 3721 GAGCGG CCGCTC(-3/-3)
BsrFI 2321 ACCGGC [GA]^CCGG[CT]
BssSI 3545 CTCGTG CACGAG(-5/-1)
BstAPI 3082 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2353 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2321 ACCGGC [GA]^CCGG[CT]
Eco81I 2353 CCTGAGG CC^T[ACGT]AGG
EcoNI 2027 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1522 GAATTC G^AATTC
EcoT22I 1454 ATGCAT ATGCA^T
FauI 70 GCGGG CCCGC(4/6)
Hin1I 2004 GGCGCC G[GA]^CG[CT]C
KasI 2004 GGCGCC G^GCGCC
NarI 2004 GGCGCC GG^CGCC
NruI 2699 TCGCGA TCG^CGA
NsiI 1454 ATGCAT ATGCA^T
PflFI 508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1899 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1652 GAAGAGC GCTCTTC(1/4)
ScaI 4130 AGTACT AGT^ACT
SfoI 2004 GGCGCC GGC^GCC
SspI 5424 AATATT AAT^ATT
StuI 2330 AGGCCT AGG^CCT
Tth111I 508 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 2700 CGCG CG^CG
4119 CGCG
AhdI 2815 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4164 GACTCCGAGTC
AspEI 2815 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4164 GACTCCGAGTC
AvaI 564 CTCGGG C^[CT]CG[GA]G
3605 CCCGAG
AvrII 1668 CCTAGG C^CTAGG
3349 CCTAGG
BamHI 2014 GGATCC G^GATCC
4010 GGATCC
BciVI 1605 GGATAC GTATCC(6/5)
4616 GGATAC
BclI 1347 TGATCA T^GATCA
3929 TGATCA
BcnI 2288 CCCGG CC^[GC]GG
3617 CCCGG
BglI 494 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4393 GCCCAAAGGGC
BlnI 1668 CCTAGG C^CTAGG
3349 CCTAGG
BsaBI 160 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3292 GATTTTGATC
BsoBI 564 CTCGGG C^[CT]CG[GA]G
3605 CCCGAG
BstUI 2700 CGCG CG^CG
4119 CGCG
BstXI 2210 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2483 CCAGGTTCATGG
Eam1105I 2815 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4164 GACTCCGAGTC
FbaI 1347 TGATCA T^GATCA
3929 TGATCA
HaeII 2004 GGCGCC [GA]GCGC^[CT]
2053 AGCGCC
HpaI 1789 GTTAAC GTT^AAC
5122 GTTAAC
MamI 160 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3292 GATTTTGATC
MvnI 2700 CGCG CG^CG
4119 CGCG
NciI 2288 CCCGG CC^[GC]GG
3617 CCCGG
XbaI 1839 TCTAGA T^CTAGA
4062 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspLU11I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraIII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI