##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI BcgI
BclI BfrI BglII BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BstBI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
MluI MroI NdeI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SexAI SfuI
SnaBI SpeI SspBI SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 3302 GAATTT [GA]^AATT[CT]
ApoI 3302 GAATTT [GA]^AATT[CT]
Asp700I 1600 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 904 TGCGCA TGC^GCA
AvrII 1308 CCTAGG C^CTAGG
BbrPI 1892 CACGTG CAC^GTG
BbsI 1020 GTCTTC GAAGAC(2/6)
BciVI 2051 GTATCC GTATCC(6/5)
BlnI 1308 CCTAGG C^CTAGG
BpuAI 1020 GTCTTC GAAGAC(2/6)
BsaAI 1892 CACGTG [CT]AC^GT[GA]
BsaWI 3423 ACCGGA [AT]^CCGG[AT]
BsiEI 2929 CGACCG CG[GA][CT]^CG
BsmBI 2731 GAGACG CGTCTC(1/5)
BsrDI 2132 GCAATG GCAATG(2/0)
BssSI 1882 CACGAG CACGAG(-5/-1)
BstEII 656 GGTTACC G^GT[ACGT]ACC
BstPI 656 GGTTACC G^GT[ACGT]ACC
EcoNI 1639 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 656 GGTTACC G^GT[ACGT]ACC
FspI 904 TGCGCA TGC^GCA
MfeI 1025 CAATTG C^AATTG
MunI 1025 CAATTG C^AATTG
PmaCI 1892 CACGTG CAC^GTG
PmlI 1892 CACGTG CAC^GTG
SbfI 635 CCTGCAGG CCTGCA^GG
SfiI 641 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 330 CCCGGG CCC^GGG
Sse8387I 635 CCTGCAGG CCTGCA^GG
XbaI 2475 TCTAGA T^CTAGA
XmaCI 330 CCCGGG C^CCGGG
XmaI 330 CCCGGG C^CCGGG
XmnI 1600 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 948 GGTACC G^GTACC
4713 GGTACC
AccI 1327 GTATAC GT^[AC][GT]AC
2423 GTATAC
Asp718I 948 GGTACC G^GTACC
4713 GGTACC
BamHI 1279 GGATCC G^GATCC
3426 GGATCC
BglI 642 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1538 GCCTCTGTGGC
BsaBI 1086 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3380 GATGAAAATC
BsrBI 687 GAGCGG CCGCTC(-3/-3)
996 GAGCGG
Bst1107I 1327 GTATAC GTA^TAC
2423 GTATAC
BstAPI 1687 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3944 GCATGGGATGC
BstZ17I 1327 GTATAC GTA^TAC
2423 GTATAC
EcoT22I 1105 ATGCAT ATGCA^T
2582 ATGCAT
KpnI 948 GGTACC GGTAC^C
4713 GGTACC
KspI 116 CCGCGG CCGC^GG
145 CCGCGG
MaeII 1850 ACGT A^CGT
1893 ACGT
MamI 1086 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3380 GATGAAAATC
NruI 241 TCGCGA TCG^CGA
317 TCGCGA
NsiI 1105 ATGCAT ATGCA^T
2582 ATGCAT
PshAI 52 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2173 GACACCTGTC
SacII 116 CCGCGG CCGC^GG
145 CCGCGG
SgrAI 350 CACCGGCG C[GA]^CCGG[CT]G
401 CGCCGGCG
XcmI 1971 CCAACACCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4145 CCAGTAGCTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII