################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI BcgI BclI BfrI BglII BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BstBI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI MluI MroI NdeI NotI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfuI SnaBI SpeI SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 3302 GAATTT [GA]^AATT[CT] ApoI 3302 GAATTT [GA]^AATT[CT] Asp700I 1600 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 904 TGCGCA TGC^GCA AvrII 1308 CCTAGG C^CTAGG BbrPI 1892 CACGTG CAC^GTG BbsI 1020 GTCTTC GAAGAC(2/6) BciVI 2051 GTATCC GTATCC(6/5) BlnI 1308 CCTAGG C^CTAGG BpuAI 1020 GTCTTC GAAGAC(2/6) BsaAI 1892 CACGTG [CT]AC^GT[GA] BsaWI 3423 ACCGGA [AT]^CCGG[AT] BsiEI 2929 CGACCG CG[GA][CT]^CG BsmBI 2731 GAGACG CGTCTC(1/5) BsrDI 2132 GCAATG GCAATG(2/0) BssSI 1882 CACGAG CACGAG(-5/-1) BstEII 656 GGTTACC G^GT[ACGT]ACC BstPI 656 GGTTACC G^GT[ACGT]ACC EcoNI 1639 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 656 GGTTACC G^GT[ACGT]ACC FspI 904 TGCGCA TGC^GCA MfeI 1025 CAATTG C^AATTG MunI 1025 CAATTG C^AATTG PmaCI 1892 CACGTG CAC^GTG PmlI 1892 CACGTG CAC^GTG SbfI 635 CCTGCAGG CCTGCA^GG SfiI 641 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 330 CCCGGG CCC^GGG Sse8387I 635 CCTGCAGG CCTGCA^GG XbaI 2475 TCTAGA T^CTAGA XmaCI 330 CCCGGG C^CCGGG XmaI 330 CCCGGG C^CCGGG XmnI 1600 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 948 GGTACC G^GTACC 4713 GGTACC AccI 1327 GTATAC GT^[AC][GT]AC 2423 GTATAC Asp718I 948 GGTACC G^GTACC 4713 GGTACC BamHI 1279 GGATCC G^GATCC 3426 GGATCC BglI 642 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1538 GCCTCTGTGGC BsaBI 1086 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3380 GATGAAAATC BsrBI 687 GAGCGG CCGCTC(-3/-3) 996 GAGCGG Bst1107I 1327 GTATAC GTA^TAC 2423 GTATAC BstAPI 1687 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3944 GCATGGGATGC BstZ17I 1327 GTATAC GTA^TAC 2423 GTATAC EcoT22I 1105 ATGCAT ATGCA^T 2582 ATGCAT KpnI 948 GGTACC GGTAC^C 4713 GGTACC KspI 116 CCGCGG CCGC^GG 145 CCGCGG MaeII 1850 ACGT A^CGT 1893 ACGT MamI 1086 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3380 GATGAAAATC NruI 241 TCGCGA TCG^CGA 317 TCGCGA NsiI 1105 ATGCAT ATGCA^T 2582 ATGCAT PshAI 52 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2173 GACACCTGTC SacII 116 CCGCGG CCGC^GG 145 CCGCGG SgrAI 350 CACCGGCG C[GA]^CCGG[CT]G 401 CGCCGGCG XcmI 1971 CCAACACCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4145 CCAGTAGCTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII