##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I AocI ApaLI AscI Asp700I
Asp718I AviII BfrI BglI BsiWI
BspDI BssHII BstEII BstPI Bsu36I
ClaI CpoI DrdI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SgrAI SnaBI SpeI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 4438 CGCG CG^CG
AcyI 3471 GGCGCC G[GA]^CG[CT]C
AhdI 1651 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3355 AGCGCT AGC^GCT
AseI 2750 ATTAAT AT^TAAT
AsnI 2750 ATTAAT AT^TAAT
AspEI 1651 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3508 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1430 CCTAGG C^CTAGG
BamHI 1593 GGATCC G^GATCC
BbeI 3471 GGCGCC GGCGC^C
BbrPI 1026 CACGTG CAC^GTG
BcgI 1901 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1430 CCTAGG C^CTAGG
BsaBI 2028 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3471 GGCGCC G[GA]^CG[CT]C
BsaI 3968 GGTCTC GGTCTC(1/5)
Bsp1407I 3580 TGTACA T^GTACA
BsrFI 2318 ACCGGC [GA]^CCGG[CT]
BsrGI 3580 TGTACA T^GTACA
Bst1107I 1710 GTATAC GTA^TAC
BstAPI 1126 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2458 TTCGAA TT^CGAA
BstUI 4438 CGCG CG^CG
BstZ17I 1710 GTATAC GTA^TAC
Cfr10I 2318 ACCGGC [GA]^CCGG[CT]
DraI 850 TTTAAA TTT^AAA
DraIII 289 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1896 CGGCCG C^GGCCG
Eam1105I 1651 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1896 CGGCCG C^GGCCG
Eco47III 3355 AGCGCT AGC^GCT
Eco52I 1896 CGGCCG C^GGCCG
Hin1I 3471 GGCGCC G[GA]^CG[CT]C
KasI 3471 GGCGCC G^GCGCC
MamI 2028 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2252 CAATTG C^AATTG
MunI 2252 CAATTG C^AATTG
MvnI 4438 CGCG CG^CG
NarI 3471 GGCGCC GG^CGCC
NspV 2458 TTCGAA TT^CGAA
PflFI 3508 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1026 CACGTG CAC^GTG
PmlI 1026 CACGTG CAC^GTG
PshBI 2750 ATTAAT AT^TAAT
SfoI 3471 GGCGCC GGC^GCC
SfuI 2458 TTCGAA TT^CGAA
SspBI 3580 TGTACA T^GTACA
SspI 4254 AATATT AAT^ATT
Tth111I 3508 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4046 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1710 GTATAC GT^[AC][GT]AC
2924 GTAGAC
AccIII 1844 TCCGGA T^CCGGA
2342 TCCGGA
BbsI 1215 GAAGAC GAAGAC(2/6)
3920 GTCTTC
BciVI 933 GGATAC GTATCC(6/5)
437 GTATCC
BclI 1490 TGATCA T^GATCA
2297 TGATCA
BglII 887 AGATCT A^GATCT
4588 AGATCT
BlpI 3414 GCTGAGC GC^T[ACGT]AGC
3527 GCTGAGC
Bpu1102I 3414 GCTGAGC GC^T[ACGT]AGC
3527 GCTGAGC
BpuAI 1215 GAAGAC GAAGAC(2/6)
3920 GTCTTC
BsaAI 1026 CACGTG [CT]AC^GT[GA]
1410 TACGTG
BseAI 1844 TCCGGA T^CCGGA
2342 TCCGGA
BsiEI 1896 CGGCCG CG[GA][CT]^CG
2001 CGACCG
BsmBI 1065 CGTCTC CGTCTC(1/5)
4058 CGTCTC
BspEI 1844 TCCGGA T^CCGGA
2342 TCCGGA
BspLU11I 1180 ACATGT A^CATGT
4567 ACATGT
BsrBI 3748 CCGCTC CCGCTC(-3/-3)
3530 GAGCGG
BsrDI 3309 CATTGC GCAATG(2/0)
1970 GCAATG
BstXI 1108 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2144 CCAACTCTTTGG
CelII 3414 GCTGAGC GC^T[ACGT]AGC
3527 GCTGAGC
FauI 3747 CCCGC CCCGC(4/6)
3532 GCGGG
FbaI 1490 TGATCA T^GATCA
2297 TGATCA
MroI 1844 TCCGGA T^CCGGA
2342 TCCGGA
NcoI 3871 CCATGG C^CATGG
4470 CCATGG
PflMI 2517 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACATCCTGG
PshAI 1378 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4052 GACACCCGTC
SbfI 1938 CCTGCAGG CCTGCA^GG
2466 CCTGCAGG
ScaI 3329 AGTACT AGT^ACT
4484 AGTACT
SphI 1167 GCATGC GCATG^C
3676 GCATGC
Sse8387I 1938 CCTGCAGG CCTGCA^GG
2466 CCTGCAGG
Van91I 2517 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI