################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII ClaI CpoI DraIII DsaI EagI EclXI Eco52I EcoRI EcoT22I FseI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SfiI SfoI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 4795 GACGTC GACGT^C AccII 1673 CGCG CG^CG AhdI 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4043 TGCGCA TGC^GCA AvrII 3186 CCTAGG C^CTAGG BalI 252 TGGCCA TGG^CCA BanI 4166 GGTGCC G^G[CT][GA]CC BclI 3506 TGATCA T^GATCA BlnI 3186 CCTAGG C^CTAGG BlpI 4971 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4971 GCTAAGC GC^T[ACGT]AGC BsmI 3412 GAATGC GAATGC(1/-1) BspHI 3661 TCATGA T^CATGA Bst1107I 586 GTATAC GTA^TAC BstAPI 3711 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3088 TTCGAA TT^CGAA BstEII 2478 GGTTACC G^GT[ACGT]ACC BstPI 2478 GGTTACC G^GT[ACGT]ACC BstUI 1673 CGCG CG^CG BstXI 2595 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 586 GTATAC GTA^TAC CelII 4971 GCTAAGC GC^T[ACGT]AGC Eam1105I 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2478 GGTTACC G^GT[ACGT]ACC EcoRV 4517 GATATC GAT^ATC FbaI 3506 TGATCA T^GATCA FspI 4043 TGCGCA TGC^GCA MluNI 252 TGGCCA TGG^CCA MscI 252 TGGCCA TGG^CCA MslI 3446 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1673 CGCG CG^CG NspV 3088 TTCGAA TT^CGAA PflFI 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2677 GACGTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3661 TCATGA T^CATGA SalI 5110 GTCGAC G^TCGAC SapI 4612 GCTCTTC GCTCTTC(1/4) SbfI 4086 CCTGCAGG CCTGCA^GG SexAI 4021 ACCTGGT A^CC[AT]GGT SfuI 3088 TTCGAA TT^CGAA Sse8387I 4086 CCTGCAGG CCTGCA^GG SspI 4065 AATATT AAT^ATT Tth111I 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 600 AAATTT [GA]^AATT[CT] 5571 AAATTC AcyI 2083 GACGCC G[GA]^CG[CT]C 4795 GACGTC ApaI 1912 GGGCCC GGGCC^C 4558 GGGCCC ApoI 600 AAATTT [GA]^AATT[CT] 5571 AAATTC AspHI 2090 GAGCTC G[AT]GC[AT]^C 4610 GAGCTC BsaHI 2083 GACGCC G[GA]^CG[CT]C 4795 GACGTC BsiHKAI 2090 GAGCTC G[AT]GC[AT]^C 4610 GAGCTC BsmBI 2459 GAGACG CGTCTC(1/5) 2714 GAGACG Bsp1407I 1250 TGTACA T^GTACA 5727 TGTACA BsrDI 301 CATTGC GCAATG(2/0) 1486 CATTGC BsrGI 1250 TGTACA T^GTACA 5727 TGTACA BssSI 2446 CACGAG CACGAG(-5/-1) 2323 CTCGTG DraI 1026 TTTAAA TTT^AAA 5718 TTTAAA DrdI 1624 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1744 GACAGAGGAGTC EcoNI 4419 CCTGTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5617 CCTGAAAGAGG Hin1I 2083 GACGCC G[GA]^CG[CT]C 4795 GACGTC PaeR7I 2620 CTCGAG C^TCGAG 3196 CTCGAG PflMI 5019 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5262 CCATAATCTGG SacI 2090 GAGCTC GAGCT^C 4610 GAGCTC ScaI 638 AGTACT AGT^ACT 659 AGTACT SphI 61 GCATGC GCATG^C 4492 GCATGC SspBI 1250 TGTACA T^GTACA 5727 TGTACA Van91I 5019 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5262 CCATAATCTGG XhoI 2620 CTCGAG C^TCGAG 3196 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AocI Aor51HI Asp700I AvaI AvaII BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco47III Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII XmnI