##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BsaAI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BssHII ClaI
CpoI DraIII DsaI EagI EclXI
Eco52I EcoRI EcoT22I FseI HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SfiI SfoI SgrAI
SmaI SnaBI SpeI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 4795 GACGTC GACGT^C
AccII 1673 CGCG CG^CG
AhdI 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4043 TGCGCA TGC^GCA
AvrII 3186 CCTAGG C^CTAGG
BalI 252 TGGCCA TGG^CCA
BanI 4166 GGTGCC G^G[CT][GA]CC
BclI 3506 TGATCA T^GATCA
BlnI 3186 CCTAGG C^CTAGG
BlpI 4971 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4971 GCTAAGC GC^T[ACGT]AGC
BsmI 3412 GAATGC GAATGC(1/-1)
BspHI 3661 TCATGA T^CATGA
Bst1107I 586 GTATAC GTA^TAC
BstAPI 3711 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3088 TTCGAA TT^CGAA
BstEII 2478 GGTTACC G^GT[ACGT]ACC
BstPI 2478 GGTTACC G^GT[ACGT]ACC
BstUI 1673 CGCG CG^CG
BstXI 2595 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 586 GTATAC GTA^TAC
CelII 4971 GCTAAGC GC^T[ACGT]AGC
Eam1105I 5219 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2478 GGTTACC G^GT[ACGT]ACC
EcoRV 4517 GATATC GAT^ATC
FbaI 3506 TGATCA T^GATCA
FspI 4043 TGCGCA TGC^GCA
MluNI 252 TGGCCA TGG^CCA
MscI 252 TGGCCA TGG^CCA
MslI 3446 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1673 CGCG CG^CG
NspV 3088 TTCGAA TT^CGAA
PflFI 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2677 GACGTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3661 TCATGA T^CATGA
SalI 5110 GTCGAC G^TCGAC
SapI 4612 GCTCTTC GCTCTTC(1/4)
SbfI 4086 CCTGCAGG CCTGCA^GG
SexAI 4021 ACCTGGT A^CC[AT]GGT
SfuI 3088 TTCGAA TT^CGAA
Sse8387I 4086 CCTGCAGG CCTGCA^GG
SspI 4065 AATATT AAT^ATT
Tth111I 2060 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 600 AAATTT [GA]^AATT[CT]
5571 AAATTC
AcyI 2083 GACGCC G[GA]^CG[CT]C
4795 GACGTC
ApaI 1912 GGGCCC GGGCC^C
4558 GGGCCC
ApoI 600 AAATTT [GA]^AATT[CT]
5571 AAATTC
AspHI 2090 GAGCTC G[AT]GC[AT]^C
4610 GAGCTC
BsaHI 2083 GACGCC G[GA]^CG[CT]C
4795 GACGTC
BsiHKAI 2090 GAGCTC G[AT]GC[AT]^C
4610 GAGCTC
BsmBI 2459 GAGACG CGTCTC(1/5)
2714 GAGACG
Bsp1407I 1250 TGTACA T^GTACA
5727 TGTACA
BsrDI 301 CATTGC GCAATG(2/0)
1486 CATTGC
BsrGI 1250 TGTACA T^GTACA
5727 TGTACA
BssSI 2446 CACGAG CACGAG(-5/-1)
2323 CTCGTG
DraI 1026 TTTAAA TTT^AAA
5718 TTTAAA
DrdI 1624 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1744 GACAGAGGAGTC
EcoNI 4419 CCTGTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5617 CCTGAAAGAGG
Hin1I 2083 GACGCC G[GA]^CG[CT]C
4795 GACGTC
PaeR7I 2620 CTCGAG C^TCGAG
3196 CTCGAG
PflMI 5019 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5262 CCATAATCTGG
SacI 2090 GAGCTC GAGCT^C
4610 GAGCTC
ScaI 638 AGTACT AGT^ACT
659 AGTACT
SphI 61 GCATGC GCATG^C
4492 GCATGC
SspBI 1250 TGTACA T^GTACA
5727 TGTACA
Van91I 5019 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5262 CCATAATCTGG
XhoI 2620 CTCGAG C^TCGAG
3196 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI Aor51HI Asp700I AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco47III
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI