##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI AspEI AviII AvrII BbeI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRV
FseI FspI HincII HindII HpaI
KasI MamI MluI MroI NarI
NotI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 474 GGTACC G^GTACC
AccI 4399 GTAGAC GT^[AC][GT]AC
Aor51HI 3049 AGCGCT AGC^GCT
ApaI 1823 GGGCCC GGGCC^C
Asp718I 474 GGTACC G^GTACC
AspI 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3119 CCCGGG C^[CT]CG[GA]G
BamHI 3772 GGATCC G^GATCC
BcgI 286 CGAAGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2765 GTATCC GTATCC(6/5)
BclI 2981 TGATCA T^GATCA
BglI 3420 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 3119 CCCGGG C^[CT]CG[GA]G
BspMI 2508 ACCTGC ACCTGC(4/8)
BssSI 4665 CTCGTG CACGAG(-5/-1)
Eco47III 3049 AGCGCT AGC^GCT
EcoNI 4128 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 446 GAATTC G^AATTC
FbaI 2981 TGATCA T^GATCA
HaeII 3049 AGCGCT [GA]GCGC^[CT]
KpnI 474 GGTACC GGTAC^C
KspI 184 CCGCGG CCGC^GG
MfeI 4365 CAATTG C^AATTG
MunI 4365 CAATTG C^AATTG
NaeI 176 GCCGGC GCC^GGC
NdeI 2952 CATATG CA^TATG
NgoMIV 176 GCCGGC G^CCGGC
NheI 4108 GCTAGC G^CTAGC
NruI 217 TCGCGA TCG^CGA
PflFI 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1553 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1233 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 184 CCGCGG CCGC^GG
ScaI 2808 AGTACT AGT^ACT
SmaI 3119 CCCGGG CCC^GGG
SpeI 4761 ACTAGT A^CTAGT
SphI 2676 GCATGC GCATG^C
StuI 58 AGGCCT AGG^CCT
Tth111I 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1553 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 811 CCACCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3119 CCCGGG C^CCGGG
XmaI 3119 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 646 GTGCAC G^TGCAC
1330 GTGCAC
ApaLI 646 GTGCAC G^TGCAC
1330 GTGCAC
BbvCI 1521 GCTGAGG CCTCAGC(-5/-2)
3510 GCTGAGG
BsmBI 3163 CGTCTC CGTCTC(1/5)
117 GAGACG
Bsp1407I 3866 TGTACA T^GTACA
3892 TGTACA
BspHI 1241 TCATGA T^CATGA
4303 TCATGA
BsrBI 1203 CCGCTC CCGCTC(-3/-3)
3748 GAGCGG
BsrFI 176 GCCGGC [GA]^CCGG[CT]
3185 GCCGGT
BsrGI 3866 TGTACA T^GTACA
3892 TGTACA
BstEII 803 GGTGACC G^GT[ACGT]ACC
4309 GGTTACC
BstPI 803 GGTGACC G^GT[ACGT]ACC
4309 GGTTACC
BstXI 758 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
814 CCAAGGAGGTGG
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
3185 GCCGGT
EcoO65I 803 GGTGACC G^GT[ACGT]ACC
4309 GGTTACC
HindIII 314 AAGCTT A^AGCTT
1011 AAGCTT
NcoI 1582 CCATGG C^CATGG
2459 CCATGG
RcaI 1241 TCATGA T^CATGA
4303 TCATGA
SexAI 2277 ACCAGGT A^CC[AT]GGT
3722 ACCTGGT
SmlI 373 CTCAAG C^T[CT][GA]AG
3298 CTCAAG
SspBI 3866 TGTACA T^GTACA
3892 TGTACA
SspI 4016 AATATT AAT^ATT
4887 AATATT
XbaI 3357 TCTAGA T^CTAGA
3450 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI