################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI AspEI AviII AvrII BbeI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRV FseI FspI HincII HindII HpaI KasI MamI MluI MroI NarI NotI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 474 GGTACC G^GTACC AccI 4399 GTAGAC GT^[AC][GT]AC Aor51HI 3049 AGCGCT AGC^GCT ApaI 1823 GGGCCC GGGCC^C Asp718I 474 GGTACC G^GTACC AspI 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3119 CCCGGG C^[CT]CG[GA]G BamHI 3772 GGATCC G^GATCC BcgI 286 CGAAGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2765 GTATCC GTATCC(6/5) BclI 2981 TGATCA T^GATCA BglI 3420 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsoBI 3119 CCCGGG C^[CT]CG[GA]G BspMI 2508 ACCTGC ACCTGC(4/8) BssSI 4665 CTCGTG CACGAG(-5/-1) Eco47III 3049 AGCGCT AGC^GCT EcoNI 4128 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 446 GAATTC G^AATTC FbaI 2981 TGATCA T^GATCA HaeII 3049 AGCGCT [GA]GCGC^[CT] KpnI 474 GGTACC GGTAC^C KspI 184 CCGCGG CCGC^GG MfeI 4365 CAATTG C^AATTG MunI 4365 CAATTG C^AATTG NaeI 176 GCCGGC GCC^GGC NdeI 2952 CATATG CA^TATG NgoMIV 176 GCCGGC G^CCGGC NheI 4108 GCTAGC G^CTAGC NruI 217 TCGCGA TCG^CGA PflFI 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1553 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1233 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 184 CCGCGG CCGC^GG ScaI 2808 AGTACT AGT^ACT SmaI 3119 CCCGGG CCC^GGG SpeI 4761 ACTAGT A^CTAGT SphI 2676 GCATGC GCATG^C StuI 58 AGGCCT AGG^CCT Tth111I 1536 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1553 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 811 CCACCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 3119 CCCGGG C^CCGGG XmaI 3119 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 646 GTGCAC G^TGCAC 1330 GTGCAC ApaLI 646 GTGCAC G^TGCAC 1330 GTGCAC BbvCI 1521 GCTGAGG CCTCAGC(-5/-2) 3510 GCTGAGG BsmBI 3163 CGTCTC CGTCTC(1/5) 117 GAGACG Bsp1407I 3866 TGTACA T^GTACA 3892 TGTACA BspHI 1241 TCATGA T^CATGA 4303 TCATGA BsrBI 1203 CCGCTC CCGCTC(-3/-3) 3748 GAGCGG BsrFI 176 GCCGGC [GA]^CCGG[CT] 3185 GCCGGT BsrGI 3866 TGTACA T^GTACA 3892 TGTACA BstEII 803 GGTGACC G^GT[ACGT]ACC 4309 GGTTACC BstPI 803 GGTGACC G^GT[ACGT]ACC 4309 GGTTACC BstXI 758 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 814 CCAAGGAGGTGG Cfr10I 176 GCCGGC [GA]^CCGG[CT] 3185 GCCGGT EcoO65I 803 GGTGACC G^GT[ACGT]ACC 4309 GGTTACC HindIII 314 AAGCTT A^AGCTT 1011 AAGCTT NcoI 1582 CCATGG C^CATGG 2459 CCATGG RcaI 1241 TCATGA T^CATGA 4303 TCATGA SexAI 2277 ACCAGGT A^CC[AT]GGT 3722 ACCTGGT SmlI 373 CTCAAG C^T[CT][GA]AG 3298 CTCAAG SspBI 3866 TGTACA T^GTACA 3892 TGTACA SspI 4016 AATATT AAT^ATT 4887 AATATT XbaI 3357 TCTAGA T^CTAGA 3450 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI