##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AvrII
BbeI BbrPI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsoBI BspDI BssHII
BssSI BstEII BstPI CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SfiI SfoI SmaI SnaBI SpeI
Sse8387I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 2965 CGCG CG^CG
AccIII 3056 TCCGGA T^CCGGA
AflIII 3527 ACATGT A^C[GA][CT]GT
AgeI 2143 ACCGGT A^CCGGT
Aor51HI 356 AGCGCT AGC^GCT
ApaI 3371 GGGCCC GGGCC^C
AviII 3522 TGCGCA TGC^GCA
BalI 2829 TGGCCA TGG^CCA
BamHI 1100 GGATCC G^GATCC
BbsI 3280 GTCTTC GAAGAC(2/6)
BbvCI 487 CCTCAGC CCTCAGC(-5/-2)
BcgI 475 GCAGTCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 3280 GTCTTC GAAGAC(2/6)
BsaBI 3251 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3056 TCCGGA T^CCGGA
BsmBI 3322 GAGACG CGTCTC(1/5)
Bsp1407I 2531 TGTACA T^GTACA
BspEI 3056 TCCGGA T^CCGGA
BspLU11I 3527 ACATGT A^CATGT
BsrGI 2531 TGTACA T^GTACA
Bst1107I 3757 GTATAC GTA^TAC
BstBI 1637 TTCGAA TT^CGAA
BstUI 2965 CGCG CG^CG
BstZ17I 3757 GTATAC GTA^TAC
Eco47III 356 AGCGCT AGC^GCT
EcoRI 3604 GAATTC G^AATTC
EcoT22I 405 ATGCAT ATGCA^T
FspI 3522 TGCGCA TGC^GCA
MamI 3251 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1703 CAATTG C^AATTG
MluNI 2829 TGGCCA TGG^CCA
MroI 3056 TCCGGA T^CCGGA
MscI 2829 TGGCCA TGG^CCA
MunI 1703 CAATTG C^AATTG
MvnI 2965 CGCG CG^CG
NsiI 405 ATGCAT ATGCA^T
NspV 1637 TTCGAA TT^CGAA
PinAI 2143 ACCGGT A^CCGGT
SexAI 3897 ACCTGGT A^CC[AT]GGT
SfuI 1637 TTCGAA TT^CGAA
SgrAI 2142 CACCGGTG C[GA]^CCGG[CT]G
SphI 86 GCATGC GCATG^C
SspBI 2531 TGTACA T^GTACA
SspI 2407 AATATT AAT^ATT
SwaI 3252 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 3439 AAATTT [GA]^AATT[CT]
3604 GAATTC
ApoI 3439 AAATTT [GA]^AATT[CT]
3604 GAATTC
Asp700I 331 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3150 GAAGCACTTC
AspI 1582 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4224 GACAATGTC
BanI 540 GGTGCC G^G[CT][GA]CC
3901 GGTGCC
BglI 90 GCCCAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3496 GCCTTGCTGGC
BsaWI 2143 ACCGGT [AT]^CCGG[AT]
3056 TCCGGA
BseRI 497 CTCCTC GAGGAG(10/8)
1174 CTCCTC
BsgI 1868 CTGCAC GTGCAG(16/14)
3481 CTGCAC
BspHI 434 TCATGA T^CATGA
1563 TCATGA
BsrBI 561 CCGCTC CCGCTC(-3/-3)
900 CCGCTC
BstAPI 3377 GCAATTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3591 GCAGACCCTGC
DraI 3253 TTTAAA TTT^AAA
4021 TTTAAA
EcoT14I 2205 CCATGG C^C[AT][AT]GG
2462 CCATGG
FauI 3374 CCCGC CCCGC(4/6)
1147 GCGGG
HincII 949 GTTGAC GT[CT]^[GA]AC
3403 GTCAAC
HindII 949 GTTGAC GT[CT]^[GA]AC
3403 GTCAAC
NcoI 2205 CCATGG C^CATGG
2462 CCATGG
PflFI 1582 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4224 GACAATGTC
RcaI 434 TCATGA T^CATGA
1563 TCATGA
SapI 1303 GAAGAGC GCTCTTC(1/4)
3267 GCTCTTC
StuI 2046 AGGCCT AGG^CCT
3013 AGGCCT
StyI 2205 CCATGG C^C[AT][AT]GG
2462 CCATGG
Tth111I 1582 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4224 GACAATGTC
XmnI 331 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3150 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BanII
BbvI BclI BcnI BfaI BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII