##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BbeI BfrI BlnI BsaI BsiWI
BspDI BspLU11I BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HpaI KasI KspI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PpuMI
PshAI Psp1406I RsrII SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 661 GGTACC G^GTACC
AccI 3644 GTATAC GT^[AC][GT]AC
AccII 48 CGCG CG^CG
AccIII 541 TCCGGA T^CCGGA
Alw44I 102 GTGCAC G^TGCAC
AlwNI 3557 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3652 CCTCAGG CC^T[ACGT]AGG
ApaI 1644 GGGCCC GGGCC^C
ApaLI 102 GTGCAC G^TGCAC
Asp700I 241 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 661 GGTACC G^GTACC
AspI 402 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4615 CTCGAG C^[CT]CG[GA]G
BanI 661 GGTACC G^G[CT][GA]CC
BbrPI 206 CACGTG CAC^GTG
BcgI 4134 GCAATTCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2565 GGATAC GTATCC(6/5)
BglII 994 AGATCT A^GATCT
BlpI 3236 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3236 GCTAAGC GC^T[ACGT]AGC
BsaAI 206 CACGTG [CT]AC^GT[GA]
BsaWI 541 TCCGGA [AT]^CCGG[AT]
BseAI 541 TCCGGA T^CCGGA
BsiEI 1197 CGATCG CG[GA][CT]^CG
BsmBI 2103 CGTCTC CGTCTC(1/5)
BsoBI 4615 CTCGAG C^[CT]CG[GA]G
BspEI 541 TCCGGA T^CCGGA
BsrDI 2323 CATTGC GCAATG(2/0)
Bst1107I 3644 GTATAC GTA^TAC
BstUI 48 CGCG CG^CG
BstXI 416 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3644 GTATAC GTA^TAC
Bsu36I 3652 CCTCAGG CC^T[ACGT]AGG
CelII 3236 GCTAAGC GC^T[ACGT]AGC
DrdI 1011 GACCCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2764 CCACGG C^C[GA][CT]GG
Eco81I 3652 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1389 ATGCAT ATGCA^T
HincII 408 GTCAAC GT[CT]^[GA]AC
HindII 408 GTCAAC GT[CT]^[GA]AC
KpnI 661 GGTACC GGTAC^C
MluI 47 ACGCGT A^CGCGT
MroI 541 TCCGGA T^CCGGA
MvnI 48 CGCG CG^CG
NdeI 2970 CATATG CA^TATG
NsiI 1389 ATGCAT ATGCA^T
NspI 826 GCATGC [GA]CATG^[CT]
PaeR7I 4615 CTCGAG C^TCGAG
PflFI 402 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 206 CACGTG CAC^GTG
PmlI 206 CACGTG CAC^GTG
PvuI 1197 CGATCG CGAT^CG
SacI 2821 GAGCTC GAGCT^C
SexAI 3307 ACCAGGT A^CC[AT]GGT
SphI 826 GCATGC GCATG^C
SwaI 4867 ATTTAAAT ATTT^AAAT
Tth111I 402 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2429 TCTAGA T^CTAGA
XhoI 4615 CTCGAG C^TCGAG
XmnI 241 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 438 GACGCC G[GA]^CG[CT]C
3124 GACGCC
AflIII 47 ACGCGT A^C[GA][CT]GT
205 ACACGT
BamHI 581 GGATCC G^GATCC
2913 GGATCC
BbvCI 921 CCTCAGC CCTCAGC(-5/-2)
1520 GCTGAGG
BcnI 65 CCGGG CC^[GC]GG
2953 CCGGG
BglI 690 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1486 GCCGTGCAGGC
BsaBI 1172 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2914 GATCCCTATC
BsaHI 438 GACGCC G[GA]^CG[CT]C
3124 GACGCC
Bsp1407I 3017 TGTACA T^GTACA
3456 TGTACA
BspHI 805 TCATGA T^CATGA
2264 TCATGA
BsrBI 4329 CCGCTC CCGCTC(-3/-3)
235 GAGCGG
BsrGI 3017 TGTACA T^GTACA
3456 TGTACA
BstAPI 781 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2401 GCATTGGCTGC
DraII 1441 AGGCCCT [GA]G^G[ACGT]CC[CT]
1644 GGGCCCT
DraIII 1051 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2879 CACCGAGTG
EcoO109I 1441 AGGCCCT [GA]G^G[ACGT]CC[CT]
1644 GGGCCCT
EcoRI 1344 GAATTC G^AATTC
3092 GAATTC
FauI 237 GCGGG CCCGC(4/6)
532 GCGGG
Hin1I 438 GACGCC G[GA]^CG[CT]C
3124 GACGCC
HindIII 2528 AAGCTT A^AGCTT
5365 AAGCTT
MaeII 207 ACGT A^CGT
1661 ACGT
MamI 1172 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2914 GATCCCTATC
MfeI 2594 CAATTG C^AATTG
5175 CAATTG
MunI 2594 CAATTG C^AATTG
5175 CAATTG
NciI 65 CCGGG CC^[GC]GG
2953 CCGGG
RcaI 805 TCATGA T^CATGA
2264 TCATGA
SbfI 1445 CCTGCAGG CCTGCA^GG
3194 CCTGCAGG
SmlI 2731 CTCAAG C^T[CT][GA]AG
4615 CTCGAG
Sse8387I 1445 CCTGCAGG CCTGCA^GG
3194 CCTGCAGG
SspBI 3017 TGTACA T^GTACA
3456 TGTACA
StuI 793 AGGCCT AGG^CCT
1271 AGGCCT
XcmI 413 CCACCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
416 CCACAGCCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BalI BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoNI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII