##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AhdI Aor51HI ApaI AscI AspEI
BamHI BsaBI Bsp1407I BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FseI HindIII HpaI MamI MfeI
MluI MunI NruI NspV PacI
PaeR7I PmeI PvuI SalI SfiI
SfuI SgrAI SmaI SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2660 GACGTC GACGT^C
AccIII 1150 TCCGGA T^CCGGA
AclI 4443 AACGTT AA^CGTT
AflII 1502 CTTAAG C^TTAAG
AgeI 1802 ACCGGT A^CCGGT
Alw44I 3262 GTGCAC G^TGCAC
AocI 2392 CCTCAGG CC^T[ACGT]AGG
ApaLI 3262 GTGCAC G^TGCAC
AseI 4595 ATTAAT AT^TAAT
AsnI 4595 ATTAAT AT^TAAT
Asp700I 4442 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2753 CCTAGG C^CTAGG
BalI 4964 TGGCCA TGG^CCA
BbrPI 3968 CACGTG CAC^GTG
BcgI 1075 GCAATGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2903 GGATAC GTATCC(6/5)
BfrI 1502 CTTAAG C^TTAAG
BglII 2973 AGATCT A^GATCT
BlnI 2753 CCTAGG C^CTAGG
BseAI 1150 TCCGGA T^CCGGA
BsiWI 2116 CGTACG C^GTACG
BspDI 1587 ATCGAT AT^CGAT
BspEI 1150 TCCGGA T^CCGGA
BspHI 826 TCATGA T^CATGA
BspMI 1618 GCAGGT ACCTGC(4/8)
BssHII 2061 GCGCGC G^CGCGC
Bsu36I 2392 CCTCAGG CC^T[ACGT]AGG
ClaI 1587 ATCGAT AT^CGAT
CpoI 1152 CGGACCG CG^G[AT]CCG
Eco81I 2392 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3875 ATGCAT ATGCA^T
KspI 874 CCGCGG CCGC^GG
MluNI 4964 TGGCCA TGG^CCA
MroI 1150 TCCGGA T^CCGGA
MscI 4964 TGGCCA TGG^CCA
NcoI 1475 CCATGG C^CATGG
NdeI 3146 CATATG CA^TATG
NsiI 3875 ATGCAT ATGCA^T
PinAI 1802 ACCGGT A^CCGGT
PmaCI 3968 CACGTG CAC^GTG
PmlI 3968 CACGTG CAC^GTG
PshBI 4595 ATTAAT AT^TAAT
Psp1406I 4443 AACGTT AA^CGTT
RcaI 826 TCATGA T^CATGA
RsrII 1152 CGGACCG CG^G[AT]CCG
SacII 874 CCGCGG CCGC^GG
SbfI 965 CCTGCAGG CCTGCA^GG
SnaBI 2785 TACGTA TAC^GTA
SpeI 5225 ACTAGT A^CTAGT
Sse8387I 965 CCTGCAGG CCTGCA^GG
SspI 4700 AATATT AAT^ATT
XcmI 1123 CCAAGTCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4442 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2626 GTAGAC GT^[AC][GT]AC
4066 GTAGAC
AlwI 320 GATCC GGATC(4/5)
568 GGATC
AlwNI 515 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
5187 CAGGTCCTG
AspI 1423 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3677 GACCTTGTC
AvaI 273 CCCGAG C^[CT]CG[GA]G
2248 CCCGAG
BglI 59 GCCGGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
885 GCCTCTTCGGC
BlpI 2326 GCTGAGC GC^T[ACGT]AGC
3700 GCTCAGC
Bpu1102I 2326 GCTGAGC GC^T[ACGT]AGC
3700 GCTCAGC
BsmBI 1421 GAGACG CGTCTC(1/5)
3849 GAGACG
BsmI 2423 GCATTC GAATGC(1/-1)
4027 GCATTC
BsoBI 273 CCCGAG C^[CT]CG[GA]G
2248 CCCGAG
BspLU11I 2780 ACATGT A^CATGT
5036 ACATGT
BstAPI 173 GCAAACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2155 GCACACGCTGC
BstYI 319 AGATCC [GA]^GATC[CT]
2973 AGATCT
CelII 2326 GCTGAGC GC^T[ACGT]AGC
3700 GCTCAGC
DrdI 2600 GACCTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4864 GACCAGGTTGTC
MflI 319 AGATCC [GA]^GATC[CT]
2973 AGATCT
NheI 3916 GCTAGC G^CTAGC
4836 GCTAGC
NotI 870 GCGGCCGC GC^GGCCGC
876 GCGGCCGC
PflFI 1423 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3677 GACCTTGTC
PflMI 949 CCAACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1123 CCAAGTCCTGG
PshAI 2142 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4069 GACAGAAGTC
SexAI 519 ACCTGGT A^CC[AT]GGT
4865 ACCAGGT
SmlI 431 CTTGAG C^T[CT][GA]AG
1502 CTTAAG
StuI 1486 AGGCCT AGG^CCT
1571 AGGCCT
Tth111I 1423 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3677 GACCTTGTC
Van91I 949 CCAACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1123 CCAAGTCCTGG
XhoII 319 AGATCC [GA]^GATC[CT]
2973 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI ApoI Asp718I
AspHI AvaII AviII BanI BanII
BbeI BbsI BbvCI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfoI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI