##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflII AgeI AocI Aor51HI
AscI AspI AviII BfrI BsiWI
BsmBI BspDI BsrDI Bsu36I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI FspI
KspI MfeI MunI NdeI NheI
NotI PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1733 GGTACC G^GTACC
AccIII 2086 TCCGGA T^CCGGA
Alw44I 1762 GTGCAC G^TGCAC
ApaLI 1762 GTGCAC G^TGCAC
AseI 1598 ATTAAT AT^TAAT
AsnI 1598 ATTAAT AT^TAAT
Asp718I 1733 GGTACC G^GTACC
AvrII 5019 CCTAGG C^CTAGG
BamHI 791 GGATCC G^GATCC
BbeI 39 GGCGCC GGCGC^C
BbrPI 3978 CACGTG CAC^GTG
BcgI 2438 GCAGACCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 131 GCCCGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5019 CCTAGG C^CTAGG
BsaAI 3978 CACGTG [CT]AC^GT[GA]
BsaBI 5010 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2086 TCCGGA [AT]^CCGG[AT]
BseAI 2086 TCCGGA T^CCGGA
BsiEI 296 CGACCG CG[GA][CT]^CG
BspEI 2086 TCCGGA T^CCGGA
Bst1107I 5609 GTATAC GTA^TAC
BstBI 6304 TTCGAA TT^CGAA
BstZ17I 5609 GTATAC GTA^TAC
DrdI 2268 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 39 GGCGCC [GA]GCGC^[CT]
HindIII 2886 AAGCTT A^AGCTT
KasI 39 GGCGCC G^GCGCC
KpnI 1733 GGTACC GGTAC^C
MamI 5010 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2756 ACGCGT A^CGCGT
MroI 2086 TCCGGA T^CCGGA
NaeI 6150 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NcoI 5488 CCATGG C^CATGG
NgoMIV 6150 GCCGGC G^CCGGC
NruI 3796 TCGCGA TCG^CGA
NspV 6304 TTCGAA TT^CGAA
PaeR7I 525 CTCGAG C^TCGAG
PmaCI 3978 CACGTG CAC^GTG
PmlI 3978 CACGTG CAC^GTG
PshBI 1598 ATTAAT AT^TAAT
SalI 1314 GTCGAC G^TCGAC
ScaI 1093 AGTACT AGT^ACT
SexAI 5479 ACCTGGT A^CC[AT]GGT
SfoI 39 GGCGCC GGC^GCC
SfuI 6304 TTCGAA TT^CGAA
SspI 1522 AATATT AAT^ATT
StuI 5004 AGGCCT AGG^CCT
SwaI 5716 ATTTAAAT ATTT^AAAT
XhoI 525 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 2914 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4450 GACAGTTTGTC
ApaI 416 GGGCCC GGGCC^C
4418 GGGCCC
Asp700I 1415 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3385 GAAGAGATTC
AspEI 2914 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4450 GACAGTTTGTC
BalI 5452 TGGCCA TGG^CCA
5575 TGGCCA
BciVI 1236 GGATAC GTATCC(6/5)
652 GTATCC
BsmI 2481 GAATGC GAATGC(1/-1)
3021 GCATTC
BspHI 1381 TCATGA T^CATGA
5839 TCATGA
BspLU11I 1955 ACATGT A^CATGT
5377 ACATGT
BsrFI 2213 ACCGGC [GA]^CCGG[CT]
6150 GCCGGC
BstXI 2660 CCAACGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5606 CCAGTATACTGG
Cfr10I 2213 ACCGGC [GA]^CCGG[CT]
6150 GCCGGC
DraI 567 TTTAAA TTT^AAA
5717 TTTAAA
DraIII 390 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2975 CACTCGGTG
Eam1105I 2914 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4450 GACAGTTTGTC
EcoNI 5103 CCTAATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5948 CCTTCCAGAGG
EcoT22I 629 ATGCAT ATGCA^T
4656 ATGCAT
MluNI 5452 TGGCCA TGG^CCA
5575 TGGCCA
MscI 5452 TGGCCA TGG^CCA
5575 TGGCCA
NsiI 629 ATGCAT ATGCA^T
4656 ATGCAT
PflMI 1339 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6374 CCAGACCCTGG
RcaI 1381 TCATGA T^CATGA
5839 TCATGA
Van91I 1339 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6374 CCAGACCCTGG
XcmI 4162 CCAGCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5711 CCACTATTTAAATGG
XmnI 1415 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3385 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrGI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI