##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp700I AspEI
AviII BamHI BbeI BbrPI BsaAI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BstBI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 5079 GTATAC GT^[AC][GT]AC
AcyI 1145 GGCGTC G[GA]^CG[CT]C
AocI 3662 CCTCAGG CC^T[ACGT]AGG
ApaI 3041 GGGCCC GGGCC^C
AseI 4781 ATTAAT AT^TAAT
AsnI 4781 ATTAAT AT^TAAT
AvrII 4654 CCTAGG C^CTAGG
BcgI 440 CGATCGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4266 GGATAC GTATCC(6/5)
BglI 773 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4654 CCTAGG C^CTAGG
BlpI 4102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4102 GCTGAGC GC^T[ACGT]AGC
BsaBI 5111 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1145 GGCGTC G[GA]^CG[CT]C
BsaWI 3884 TCCGGT [AT]^CCGG[AT]
BsmBI 1132 CGTCTC CGTCTC(1/5)
BssSI 2921 CTCGTG CACGAG(-5/-1)
Bst1107I 5079 GTATAC GTA^TAC
BstAPI 3323 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5079 GTATAC GTA^TAC
Bsu36I 3662 CCTCAGG CC^T[ACGT]AGG
CelII 4102 GCTGAGC GC^T[ACGT]AGC
DraIII 3849 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2471 GACATGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3662 CCTCAGG CC^T[ACGT]AGG
EcoRI 1543 GAATTC G^AATTC
EcoT22I 4929 ATGCAT ATGCA^T
HaeII 1436 AGCGCC [GA]GCGC^[CT]
Hin1I 1145 GGCGTC G[GA]^CG[CT]C
HincII 4437 GTTGAC GT[CT]^[GA]AC
HindII 4437 GTTGAC GT[CT]^[GA]AC
MamI 5111 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4349 CATATG CA^TATG
NsiI 4929 ATGCAT ATGCA^T
PshBI 4781 ATTAAT AT^TAAT
PvuI 440 CGATCG CGAT^CG
SfiI 772 GGCCTCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XcmI 2387 CCAGGGGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1307 GGTACC G^GTACC
1730 GGTACC
AccII 294 CGCG CG^CG
444 CGCG
AclI 5717 AACGTT AA^CGTT
6014 AACGTT
Asp718I 1307 GGTACC G^GTACC
1730 GGTACC
AspI 54 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5026 GACAATGTC
BbvCI 2354 CCTCAGC CCTCAGC(-5/-2)
798 GCTGAGG
BclI 1500 TGATCA T^GATCA
5110 TGATCA
BglII 3187 AGATCT A^GATCT
3929 AGATCT
BsiEI 440 CGATCG CG[GA][CT]^CG
1509 CGGTCG
BsmI 4525 GCATTC GAATGC(1/-1)
5564 GCATTC
BstUI 294 CGCG CG^CG
444 CGCG
CfoI 1437 GCGC GCG^C
2616 GCGC
FauI 1231 CCCGC CCCGC(4/6)
283 GCGGG
FbaI 1500 TGATCA T^GATCA
5110 TGATCA
HhaI 1437 GCGC GCG^C
2616 GCGC
HinP1I 1437 GCGC G^CGC
2616 GCGC
HindIII 4315 AAGCTT A^AGCTT
5252 AAGCTT
KpnI 1307 GGTACC GGTAC^C
1730 GGTACC
MvnI 294 CGCG CG^CG
444 CGCG
PaeR7I 406 CTCGAG C^TCGAG
617 CTCGAG
PflFI 54 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5026 GACAATGTC
PflMI 4256 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5432 CCAAGCTCTGG
PpuMI 2508 AGGTCCT [GA]G^G[AT]CC[CT]
2569 AGGTCCT
Psp1406I 5717 AACGTT AA^CGTT
6014 AACGTT
SpeI 2632 ACTAGT A^CTAGT
4309 ACTAGT
SphI 1155 GCATGC GCATG^C
5380 GCATGC
SspI 1340 AATATT AAT^ATT
6040 AATATT
StuI 5221 AGGCCT AGG^CCT
5309 AGGCCT
Tth111I 54 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5026 GACAATGTC
Van91I 4256 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5432 CCAAGCTCTGG
XhoI 406 CTCGAG C^TCGAG
617 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII