##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BalI BbrPI BclI
BlnI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstZ17I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MluI MluNI MroI MscI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SfiI
SgrAI SpeI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 3866 GGCGCC G[GA]^CG[CT]C
AflIII 1254 ACGTGT A^C[GA][CT]GT
Alw44I 3291 GTGCAC G^TGCAC
ApaLI 3291 GTGCAC G^TGCAC
Asp700I 213 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 3866 GGCGCC G^G[CT][GA]CC
BbeI 3866 GGCGCC GGCGC^C
BcgI 1983 GCAGCTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3599 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4352 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4352 GCTCAGC GC^T[ACGT]AGC
BsaHI 3866 GGCGCC G[GA]^CG[CT]C
BsaWI 4237 TCCGGT [AT]^CCGG[AT]
BstBI 326 TTCGAA TT^CGAA
CelII 4352 GCTCAGC GC^T[ACGT]AGC
DraIII 2710 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4518 CGGCCA [CT]^GGCC[GA]
EcoRI 254 GAATTC G^AATTC
HaeII 3866 GGCGCC [GA]GCGC^[CT]
Hin1I 3866 GGCGCC G[GA]^CG[CT]C
HindIII 387 AAGCTT A^AGCTT
KasI 3866 GGCGCC G^GCGCC
KspI 3870 CCGCGG CCGC^GG
MfeI 2809 CAATTG C^AATTG
MunI 2809 CAATTG C^AATTG
NarI 3866 GGCGCC GG^CGCC
NcoI 1091 CCATGG C^CATGG
NspV 326 TTCGAA TT^CGAA
PflMI 2709 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 3870 CCGCGG CCGC^GG
ScaI 4005 AGTACT AGT^ACT
SexAI 3882 ACCTGGT A^CC[AT]GGT
SfoI 3866 GGCGCC GGC^GCC
SfuI 326 TTCGAA TT^CGAA
SmaI 2063 CCCGGG CCC^GGG
SphI 540 GCATGC GCATG^C
SspI 1207 AATATT AAT^ATT
Van91I 2709 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2063 CCCGGG C^CCGGG
XmaI 2063 CCCGGG C^CCGGG
XmnI 213 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1844 GTAGAC GT^[AC][GT]AC
4505 GTAGAC
AccII 3871 CGCG CG^CG
4073 CGCG
AcsI 254 GAATTC [GA]^AATT[CT]
4525 AAATTC
AflII 431 CTTAAG C^TTAAG
4288 CTTAAG
AhdI 756 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2816 GACAAGTTGTC
ApaI 418 GGGCCC GGGCC^C
3076 GGGCCC
ApoI 254 GAATTC [GA]^AATT[CT]
4525 AAATTC
AspEI 756 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2816 GACAAGTTGTC
AspI 3881 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3940 GACGGTGTC
BciVI 1629 GGATAC GTATCC(6/5)
4026 GTATCC
BfrI 431 CTTAAG C^TTAAG
4288 CTTAAG
BglII 484 AGATCT A^GATCT
1786 AGATCT
BsmBI 4387 GAGACG CGTCTC(1/5)
4646 GAGACG
BsmI 2139 GAATGC GAATGC(1/-1)
2681 GCATTC
BsrDI 701 GCAATG GCAATG(2/0)
1285 GCAATG
BstUI 3871 CGCG CG^CG
4073 CGCG
DraI 1175 TTTAAA TTT^AAA
1680 TTTAAA
Eam1105I 756 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2816 GACAAGTTGTC
EcoT22I 24 ATGCAT ATGCA^T
2418 ATGCAT
FauI 4071 CCCGC CCCGC(4/6)
3730 GCGGG
MvnI 3871 CGCG CG^CG
4073 CGCG
NsiI 24 ATGCAT ATGCA^T
2418 ATGCAT
PflFI 3881 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3940 GACGGTGTC
PpuMI 1325 AGGACCT [GA]G^G[AT]CC[CT]
1537 AGGACCT
PstI 1793 CTGCAG CTGCA^G
2878 CTGCAG
SacI 1245 GAGCTC GAGCT^C
2977 GAGCTC
SapI 1852 GAAGAGC GCTCTTC(1/4)
1038 GCTCTTC
SnaBI 3440 TACGTA TAC^GTA
4229 TACGTA
StuI 620 AGGCCT AGG^CCT
3597 AGGCCT
TfiI 1902 GATTC G^A[AT]TC
2268 GAATC
Tth111I 3881 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3940 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BamHI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII