##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI ApoI AscI Asp700I
AspEI BbrPI BciVI BfrI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FseI KspI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SapI SfuI SgrAI
SnaBI SpeI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 2526 TCCGGA T^CCGGA
Alw44I 251 GTGCAC G^TGCAC
Aor51HI 1668 AGCGCT AGC^GCT
ApaLI 251 GTGCAC G^TGCAC
AseI 4891 ATTAAT AT^TAAT
AsnI 4891 ATTAAT AT^TAAT
AviII 3781 TGCGCA TGC^GCA
AvrII 367 CCTAGG C^CTAGG
BclI 3354 TGATCA T^GATCA
BlnI 367 CCTAGG C^CTAGG
BseAI 2526 TCCGGA T^CCGGA
Bsp1407I 1626 TGTACA T^GTACA
BspDI 4016 ATCGAT AT^CGAT
BspEI 2526 TCCGGA T^CCGGA
BsrBI 3718 GAGCGG CCGCTC(-3/-3)
BsrDI 4692 CATTGC GCAATG(2/0)
BsrGI 1626 TGTACA T^GTACA
BstAPI 3703 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2289 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4016 ATCGAT AT^CGAT
Eco47III 1668 AGCGCT AGC^GCT
FbaI 3354 TGATCA T^GATCA
FspI 3781 TGCGCA TGC^GCA
HindIII 1999 AAGCTT A^AGCTT
HpaI 5024 GTTAAC GTT^AAC
MroI 2526 TCCGGA T^CCGGA
NheI 4243 GCTAGC G^CTAGC
PshBI 4891 ATTAAT AT^TAAT
SalI 681 GTCGAC G^TCGAC
SbfI 3058 CCTGCAGG CCTGCA^GG
ScaI 3291 AGTACT AGT^ACT
SfiI 3815 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3058 CCTGCAGG CCTGCA^GG
SspBI 1626 TGTACA T^GTACA
XbaI 4037 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3984 GGTACC G^GTACC
4672 GGTACC
AccI 681 GTCGAC GT^[AC][GT]AC
4010 GTCTAC
AcyI 2459 GGCGCC G[GA]^CG[CT]C
4868 GGCGCC
Asp718I 3984 GGTACC G^GTACC
4672 GGTACC
AspI 437 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
563 GACAGTGTC
BamHI 2119 GGATCC G^GATCC
3223 GGATCC
BbeI 2459 GGCGCC GGCGC^C
4868 GGCGCC
BbvCI 4028 GCTGAGG CCTCAGC(-5/-2)
4252 GCTGAGG
BglII 31 AGATCT A^GATCT
2759 AGATCT
BsaHI 2459 GGCGCC G[GA]^CG[CT]C
4868 GGCGCC
BssSI 1438 CACGAG CACGAG(-5/-1)
3214 CACGAG
BstEII 536 GGTGACC G^GT[ACGT]ACC
1807 GGTTACC
BstPI 536 GGTGACC G^GT[ACGT]ACC
1807 GGTTACC
EcoO65I 536 GGTGACC G^GT[ACGT]ACC
1807 GGTTACC
Hin1I 2459 GGCGCC G[GA]^CG[CT]C
4868 GGCGCC
KasI 2459 GGCGCC G^GCGCC
4868 GGCGCC
KpnI 3984 GGTACC GGTAC^C
4672 GGTACC
MaeII 950 ACGT A^CGT
3367 ACGT
MslI 3181 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4747 CATGCTGGTG
NarI 2459 GGCGCC GG^CGCC
4868 GGCGCC
PaeR7I 68 CTCGAG C^TCGAG
3320 CTCGAG
PflFI 437 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
563 GACAGTGTC
SexAI 2899 ACCTGGT A^CC[AT]GGT
3279 ACCTGGT
SfoI 2459 GGCGCC GGC^GCC
4868 GGCGCC
SphI 1734 GCATGC GCATG^C
4746 GCATGC
Tth111I 437 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
563 GACAGTGTC
XcmI 1126 CCACGCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3817 CCAGGAGGGCCCTGG
XhoI 68 CTCGAG C^TCGAG
3320 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI