##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BbeI BbrPI BbvCI
BcgI BclI BfrI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BspEI BspLU11I BsrBI BsrFI
BssHII BstBI Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 846 CGCG CG^CG
AcsI 2876 AAATTT [GA]^AATT[CT]
ApoI 2876 AAATTT [GA]^AATT[CT]
AspI 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2894 CCTAGG C^CTAGG
BamHI 5043 GGATCC G^GATCC
BlnI 2894 CCTAGG C^CTAGG
BsaWI 349 ACCGGA [AT]^CCGG[AT]
BspDI 4631 ATCGAT AT^CGAT
BssSI 4308 CACGAG CACGAG(-5/-1)
Bst1107I 243 GTATAC GTA^TAC
BstUI 846 CGCG CG^CG
BstZ17I 243 GTATAC GTA^TAC
ClaI 4631 ATCGAT AT^CGAT
DraI 5241 TTTAAA TTT^AAA
FauI 3549 GCGGG CCCGC(4/6)
HincII 2240 GTCAAC GT[CT]^[GA]AC
HindII 2240 GTCAAC GT[CT]^[GA]AC
MaeII 2064 ACGT A^CGT
MvnI 846 CGCG CG^CG
PaeR7I 3257 CTCGAG C^TCGAG
PflFI 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 4928 CAGCTG CAG^CTG
ScaI 4734 AGTACT AGT^ACT
SmaI 4698 CCCGGG CCC^GGG
Tth111I 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5117 TCTAGA T^CTAGA
XhoI 3257 CTCGAG C^TCGAG
XmaCI 4698 CCCGGG C^CCGGG
XmaI 4698 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 4066 GTGCAC G^TGCAC
4121 GTGCAC
AocI 2183 CCTCAGG CC^T[ACGT]AGG
4115 CCTCAGG
ApaLI 4066 GTGCAC G^TGCAC
4121 GTGCAC
BciVI 352 GGATAC GTATCC(6/5)
2193 GTATCC
BglII 290 AGATCT A^GATCT
4077 AGATCT
BspHI 2404 TCATGA T^CATGA
3620 TCATGA
BsrDI 4615 CATTGC GCAATG(2/0)
307 GCAATG
Bsu36I 2183 CCTCAGG CC^T[ACGT]AGG
4115 CCTCAGG
DraIII 415 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1417 CACCAGGTG
Eco81I 2183 CCTCAGG CC^T[ACGT]AGG
4115 CCTCAGG
HgaI 2542 GACGC GACGC(5/10)
3826 GACGC
HindIII 3537 AAGCTT A^AGCTT
3598 AAGCTT
NdeI 1876 CATATG CA^TATG
3201 CATATG
RcaI 2404 TCATGA T^CATGA
3620 TCATGA
SacI 1224 GAGCTC GAGCT^C
4756 GAGCTC
SapI 3337 GCTCTTC GCTCTTC(1/4)
4841 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII