################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BbrPI BbvCI BcgI BclI BfrI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspLU11I BsrBI BsrFI BssHII BstBI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccII 846 CGCG CG^CG AcsI 2876 AAATTT [GA]^AATT[CT] ApoI 2876 AAATTT [GA]^AATT[CT] AspI 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2894 CCTAGG C^CTAGG BamHI 5043 GGATCC G^GATCC BlnI 2894 CCTAGG C^CTAGG BsaWI 349 ACCGGA [AT]^CCGG[AT] BspDI 4631 ATCGAT AT^CGAT BssSI 4308 CACGAG CACGAG(-5/-1) Bst1107I 243 GTATAC GTA^TAC BstUI 846 CGCG CG^CG BstZ17I 243 GTATAC GTA^TAC ClaI 4631 ATCGAT AT^CGAT DraI 5241 TTTAAA TTT^AAA FauI 3549 GCGGG CCCGC(4/6) HincII 2240 GTCAAC GT[CT]^[GA]AC HindII 2240 GTCAAC GT[CT]^[GA]AC MaeII 2064 ACGT A^CGT MvnI 846 CGCG CG^CG PaeR7I 3257 CTCGAG C^TCGAG PflFI 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 4928 CAGCTG CAG^CTG ScaI 4734 AGTACT AGT^ACT SmaI 4698 CCCGGG CCC^GGG Tth111I 1722 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5117 TCTAGA T^CTAGA XhoI 3257 CTCGAG C^TCGAG XmaCI 4698 CCCGGG C^CCGGG XmaI 4698 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Alw44I 4066 GTGCAC G^TGCAC 4121 GTGCAC AocI 2183 CCTCAGG CC^T[ACGT]AGG 4115 CCTCAGG ApaLI 4066 GTGCAC G^TGCAC 4121 GTGCAC BciVI 352 GGATAC GTATCC(6/5) 2193 GTATCC BglII 290 AGATCT A^GATCT 4077 AGATCT BspHI 2404 TCATGA T^CATGA 3620 TCATGA BsrDI 4615 CATTGC GCAATG(2/0) 307 GCAATG Bsu36I 2183 CCTCAGG CC^T[ACGT]AGG 4115 CCTCAGG DraIII 415 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 1417 CACCAGGTG Eco81I 2183 CCTCAGG CC^T[ACGT]AGG 4115 CCTCAGG HgaI 2542 GACGC GACGC(5/10) 3826 GACGC HindIII 3537 AAGCTT A^AGCTT 3598 AAGCTT NdeI 1876 CATATG CA^TATG 3201 CATATG RcaI 2404 TCATGA T^CATGA 3620 TCATGA SacI 1224 GAGCTC GAGCT^C 4756 GAGCTC SapI 3337 GCTCTTC GCTCTTC(1/4) 4841 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII